Adipogenic potential in human mesenchymal stem cells strictly depends on adult or foetal tissue harvest

A humanized system to expand in vitro amniotic fluid-derived stem cells intended for clinical application

“…However, AFSC displayed a poor adipogenic differentiation capacity demonstrated by decreases in lipid levels low level of PPAR γ gene expression. It is important to note that AFSC present intermediate characteristic between embryonic and adult stem cells; it has been recently reported that adult MSC display higher adipose differentiation capacities than fetal MSC [31].…”

Diet composition transiently modulates proliferative and potency features of human cord blood-derived mesenchymal stem cells

“…To investigate how behavior and properties of CBMSC are influenced by medium peculiarities, we chose two extremely different media as aMEM (Ragni et al, 2013b), one of the most widely used synthetic cell culture medium, and SPE-IV (Gadelorge et al, 2014), a recently released nutrient-rich medium containing supplemental cytokines (rh-IGF-1 and rh-b-FGF) and lipids that is reported by the supplier to confer higher proliferation rates maintaining stemness and differentiation properties in different MSC types. To avoid bias introduced by clones independently isolated with the two media and potentially displaying differential features (Halleux et al, 2001), MSC were first isolated in SPE-IV due to a higher isolation rate observed for CBMSC (Barilani et al, submitted).…”

“…Isolated MSC were able to differentiate into adipogenic, osteogenic and chondrogenic lineages confirming their mesenchymal features (Ragni et al, 2013b). Briefly, cells were incubated for two weeks in differentiation media (Lonza, Basel, CH) following manufacturer's instructions.…”

Dissection of the Cord Blood Stromal Component Reveals Predictive Parameters for Culture Outcome

“…These experimental tricks may suggest that CBMSCs are less prone than MSCs from other sources to give rise to adipose tissue-differentiated cell types. In this regard, differences at molecular level between CBMSCs and BMMSCs that explain this behavior during adipogenic induction have been recently elucidated [10]. CBMSCs could lack in some measure adipogenic potential or either their being ''fetal'' cells implies also a more immature state that necessitates longer or ad hoc differentiation protocols.…”

“…These cells were first described in adult tissues [2][3][4], but in recent years, they have been found in many fetal tissues as well [5][6][7]. Thus, even if a definition for MSCs has been provided by the International Society for Cellular Therapy (ISCT) [8], this acronym comprises a wide range of cells obtained from various sources that frequently possess different biological characteristics [9][10][11].…”