Adhesive protein‐free synthetic hydrogels for retinal pigment epithelium cell culture with low ROS level

Chen, Yong Mei; Liu, Zhen Qi; Feng, Zhi Hui; Xu, Feng; Liu, Jian Kang

doi:10.1002/jbm.a.34904

Cited by 22 publications

(18 citation statements)

References 48 publications

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“…Although natural-derived hydrogels have excellent biocompatibility, the disadvantages of high batch-to-batch variations, undefined matrix compositions and restricted modification possibilities [1,30] significantly limit their applications. By contrast, synthetic hydrogels have defined chemical structures and stable physicochemical properties [31][32][33]. Unfortunately, they involve the biocompatibility issue since they lack the biologically relevant chemistries [30], and are considered as foreign object for cells.…”

Section: Introductionmentioning

confidence: 89%

Dextran-based hydrogel formed by thiol-Michael addition reaction for 3D cell encapsulation

Liu

Zhao

Zhu

et al. 2015

Colloids and Surfaces B: Biointerfaces

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Section: Introductionmentioning

confidence: 89%

Dextran-based hydrogel formed by thiol-Michael addition reaction for 3D cell encapsulation

Liu

Zhao

Zhu

et al. 2015

Colloids and Surfaces B: Biointerfaces

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“…[1][2][3][4][5][6][7][8] Recently, self-healing hydrogels capable of autonomously repairing cracks, offer substantial benefi ts to wileyonlinelibrary.com hydrogel based on reversible Diels-Alder reaction needs to synthesize specifi c diene and dienophile, [ 39 ] which may involve the potential issue of cytotoxicity. [1][2][3][4][5][6][7][8] Recently, self-healing hydrogels capable of autonomously repairing cracks, offer substantial benefi ts to wileyonlinelibrary.com hydrogel based on reversible Diels-Alder reaction needs to synthesize specifi c diene and dienophile, [ 39 ] which may involve the potential issue of cytotoxicity.…”

Section: Introductionmentioning

confidence: 99%

Novel Biocompatible Polysaccharide‐Based Self‐Healing Hydrogel

Zhao

Yang

Liu

et al. 2015

Adv Funct Materials

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1352 wileyonlinelibrary.com maintain the integrity of network structures and mechanical properties of bulk gels, leading to their long-term use with stable functionality. [9][10][11][12][13] The scientifi c community nowadays focus on two major approaches, based on dynamic covalent bond [14][15][16][17] and noncovalent bond, [18][19][20][21][22][23][24][25][26][27][28] to design self-healing hydrogels. Dynamic covalent bond integrates both the stability of covalent bond and the reversibility of noncovalent bond in one system. [ 29 ] These dynamic covalent bonds can build an intrinsic dynamic equilibrium of bond generation and dissociation in hydrogel networks, endowing self-healing performance to the hydrogels. Despite a few examples of self-healing hydrogels based on the dynamic covalent bonds (e.g., phenylboronate esters, [30][31][32] acylhydrazone bonds, [ 29,33 ] disulfi de bonds, [34][35][36] and Diels-Alder reactions, [ 37,38 ] the diffi culty of manipulating in vivo due to their nonautonomous self-healing characteristics, impedes their applications. For instance, selfhealing hydrogel based on dynamically restructuring of phenylboronic esters needs an acid environment (pH 4.2), [ 30 ] while hydrogel based on dynamic disulfi de bonds usually needs an alkali environment (pH 9), [ 34 ] to trigger the corresponding healing process. Moreover, complicated synthetic procedures and unconfi rmed biocompatibility of these self-healing hydrogels may limit their applications. For instance, the self-healing A novel biocompatible polysaccharide-based self-healing hydrogel, CEC-l-OSA-l-ADH hydrogel ("l" means "linked-by"), is developed by exploiting the dynamic reaction of N -carboxyethyl chitosan (CEC) and adipic acid dihydrazide (ADH) with oxidized sodium alginate (OSA). The self-healing ability, as demonstrated by rheological recovery, macroscopic observation, and beam-shaped strain compression measurement, is attributed to the coexistence of dynamic imine and acylhydrazone bonds in the hydrogel networks. The CEC-l-OSA-l-ADH hydrogel shows excellent self-healing ability under physiological conditions with a high healing effi ciency (up to 95%) without need for any external stimuli. In addition, the CEC-l-OSA-l-ADH hydrogel exhibits good cytocompatibility and cell release as demonstrated by threedimensional cell encapsulation. With these superior properties, the developed hydrogel holds great potential for applications in various biomedical fi elds, e.g., as cell or drug delivery carriers.

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“…Soft and biocompatible hydrogel, possessing three-dimensional (3D) polymer networks and physicochemical properties similar to the extracellular matrices of tissues891011, can provide the 3D microenvironment for the differentiation and engraftment of transplanted NSCs. Injectable hydrogel, delivering cells into localized lesion site within any defect shape via minimally invasive manner, has been extensively applied in the delivery of NSCs121314.…”

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confidence: 99%

Self-healing polysaccharide-based hydrogels as injectable carriers for neural stem cells

Zhao¹,

Zhao²,

Chen³

et al. 2016

Sci Rep

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Self-healing injectable hydrogels can be formulated as three-dimensional carriers for the treatment of neurological diseases with desirable advantages, such as avoiding the potential risks of cell loss during injection, protecting cells from the shearing force of injection. However, the demands for biocompatible self-healing injectable hydrogels to meet above requirements and to promote the differentiation of neural stem cells (NSCs) into neurons remain a challenge. Herein, we developed a biocompatible self-healing polysaccharide-based hydrogel system as a novel injectable carrier for the delivery of NSCs. N-carboxyethyl chitosan (CEC) and oxidized sodium alginate (OSA) are the main backbones of the hydrogel networks, denoted as CEC-l-OSA hydrogel (“l” means “linked-by”). Owing to the dynamic imine cross-links formed by a Schiff reaction between amino groups on CEC and aldehyde groups on OSA, the hydrogel possesses the ability to self-heal into a integrity after being injected from needles under physiological conditions. The CEC-l-OSA hydrogel in which the stiffness mimicking nature brain tissues (100~1000 Pa) can be finely tuned to support the proliferation and neuronal differentiation of NSCs. The multi-functional, injectable, and self-healing CEC-l-OSA hydrogels hold great promises for NSC transplantation and further treatment of neurological diseases.

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Adhesive protein‐free synthetic hydrogels for retinal pigment epithelium cell culture with low ROS level

Cited by 22 publications

References 48 publications

Dextran-based hydrogel formed by thiol-Michael addition reaction for 3D cell encapsulation

Dextran-based hydrogel formed by thiol-Michael addition reaction for 3D cell encapsulation

Novel Biocompatible Polysaccharide‐Based Self‐Healing Hydrogel

Self-healing polysaccharide-based hydrogels as injectable carriers for neural stem cells

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