Expression profile of oestrogen receptors and oestrogen‐related receptors is organ specific and sex dependent: the Japanese medaka Oryzias latipes model

Abstract

Gene expression of all known subtypes of oestrogen receptor (ER) and oestrogen-related receptor (ERR) in multiple organs and both sexes of the Japanese medaka Oryzias latipes was profiled and systematically analysed. As revealed by statistical analyses and low-dimensional projections, the expressions of ERRs proved to be organ and sex dependent, which is in contrast with the ubiquitous nature of ERs. Moreover, expressions of specific ERR isoforms (ERRγ1, ERRγ2) were strongly correlated with that of all ERs (ERα, ERβ1 and ERβ2), suggesting the existence of potential interactions. Findings of this study shed light on the co-regulatory role of particular ERRs in oestrogen-ERs signalling and highlight the potential importance of ERRs in determining organ and sex-specific oestrogen responses. Using O. latipes as an alternative vertebrate model, this study provides new directions that call for collective efforts from the scientific community to unravel the mechanistic action of ER-ERR cross-talks, and their intertwining functions, in a cell and sex-specific manner in vivo.

Investigation of hormone receptor expressions in the fins of Oryzias woworae (Actinopterygii: Beloniformes: Adrianichthyidae)

“…This result was incongruent with fin phenotypes, because it is thought that oestrogen might drive feminine hormones controlling the secondary characteristics of females. A well known fact in teleosts fishes is that oestrogens not only play crucial roles in reproductive functions of females but also are important in the reproductive functions of males, including the development of secondary sex characteristics and behaviour (Muramatsu and Inoue 2000, Chakraborty et al 2011, Cheung et al 2013. This result suggests that the pectoral fins of males may be the target for oestrogens, even though oestrogens affect that coordinate functions via ERα in these fins have so far been discussed as being related to sex characteristics.…”

Genetic Linkage Map and Comparative Genome Analysis for the Atlantic Killifish (<i>Fundulus heteroclitus</i>)

“…All three teleost species are euteleostein with Fundulus and medaka containing 24 chromosomes (2N = 48) and representing the subdivision neoteleostei versus zebrafish with 25 chromosomes (2N = 50) in the subdivision ostariophysi. Several comparative phylogenetic [30] and phylogenomic [31] [32] assessments of teleost fish support this taxonomic structure and relative evolutionary distances. Earlier theories explaining genome evolution suggest synteny is an artifact of ancestral linkages that have not yet been disrupted by random micro-and macro-rearrangements such as indels, inversions and translocations [33].…”

Short-term effects of estradiol and bisphenol A on gene expression associated with early head mineralization in the seabass Dicentrarchus labrax

“…The fact that EREs were found in the erb2, gperb, erra, errb2 and errga promoters supports the hypothesis for a potential crosstalk between nuclear ERs and orphan ERRs in the European seabass. Indeed, the ERRs have been postulated to modulate E2 signaling by either synergizing or competing with ERs in regulating multiple shared transcriptional targets (Horard and Vanacker, 2003;Cheung et al, 2013). In contrast to ERs, ERRs assume an active state without a ligand being bound to the ligand binding domain (LBD) (Kallen et al, 2004) which makes them constitutive transcription factors when linked to their ERR-response Element (ERRE).…”