Trabecular titanium can inducein vitroosteogenic differentiation of human adipose derived stem cells without osteogenic factors

Abstract

Trabecular Titanium (TT) is an innovative highly porous structure that imitates the morphology of trabecular bone with good mechanical properties. Adipose-derived stem cells are a multipotent cell population that can be used in regenerative medicine, in particular, for bone therapeutic applications. The ability of TT to induce the osteogenic differentiation of human adipose derived stem cells (hASCs) in the absence of osteogenic factors was evaluated using molecular biological, biochemical, and immunohistochemical methods. At 7 and 21 days from differentiation, the hASCs grown on TT scaffolds showed similar expressions of alkaline phosphatase (ALP) and Runx-2 both in the presence and in the absence of osteogenic factors, as well as at transcript and protein levels. hASCs cultured on monolayer in the presence of the medium obtained from the wells where hASCs/scaffold constructs were cultured in the absence of osteogenic factors differentiated towards the osteogenic phenotype: their gene and protein expression of ALP and Runx-2 was similar to that of the same cells cultured in the presence of osteogenic factors, and significantly higher than that of the ones cultured in growth medium.

Acetabular revision arthroplasty using trabecular titanium implants

“…TT has exceptional biomechanical characteristics [8]. In vivo studies showed promising results and remarkably fast and complete osseointegration [9]. Similar clinical results are also anticipated from Delta TT implants.…”

“…Titanium alloys have been successfully used for decades for orthopaedic and dental prostheses. TT is a specific hexagonal space porous material with high osteoinductive and osteoconductive properties and biocompatibility that lead to rapid and extensive bone ingrowth into the material [8,9]. Special attributes of TT-its porosity, high friction coefficient, tensile strength and high corrosion resistance-are extraordinary material characteristics that enable the use of TT in situations where bone defects in weight-bearing skeletal parts are required.…”

Numerical and Mechanical Analyses of a 3d-Printed Titanium Trabecular Dental Implant

“…Studies performed in-vitro (outside of the organism) show that this structure promotes osseointegration [4]. It has been reported that the integration of a trabecular implant and vascularization can occur as fast as in two weeks [5].…”

Use of Trabecular Titanium Implants for Primary Hip Arthroplasty

“…Porowaty tytan -trabecular titanium -charakteryzuje porowatość ułatwiająca tworzenie naczyń krwionośnych w nowo powstającej tkance kostnej. W badaniach in vitro wykazano stymulujące osteointegrację działanie tego materiału [12]. Rów -nież in vivo u ludzi wykazano integrację z tkanką kostną i wnikanie naczyń już po dwóch tygodniach od zaimplantowania [13] oraz szybkie przerastanie tkanką gospodarza [14].…”

“…Trabecular titanium is porous, which facilitates the formation of blood vessels in the newly formed bone tissue. In vitro studies have shown that this material stimulates osteointegration [12]. Si milarly, in vivo studies with human subjects have demonstrated integration of titanium with the bone tissue and vascularisation as soon as two weeks after the surgery [13] and early ingrowth of the host tissue [14].…”