Production, Characterization, and Antioxidant Activity of Fucoxanthin from the Marine Diatom Odontella aurita

Abstract

The production, characterization, and antioxidant capacity of the carotenoid fucoxanthin from the marine diatom Odontella aurita were investigated. The results showed that low light and nitrogen-replete culture medium enhanced the biosynthesis of fucoxanthin. The maximum biomass concentration of 6.36 g L−1 and maximum fucoxanthin concentration of 18.47 mg g−1 were obtained in cultures grown in a bubble column photobioreactor (Ø 3.0 cm inner diameter), resulting in a fucoxanthin volumetric productivity of 7.96 mg L−1 day−1. A slight reduction in biomass production was observed in the scaling up of O. aurita culture in a flat plate photobioreactor, yet yielded a comparable fucoxanthin volumetric productivity. A rapid method was developed for extraction and purification of fucoxanthin. The purified fucoxanthin was identified as all-trans-fucoxanthin, which exhibited strong antioxidant properties, with the effective concentration for 50% scavenging (EC50) of 1,1-dihpenyl-2-picrylhydrazyl (DPPH) radical and 2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid (ABTS) radical being 0.14 and 0.03 mg mL−1, respectively. Our results suggested that O. aurita can be a natural source of fucoxanthin for human health and nutrition.

Environmental and nutrient conditions influence fucoxanthin productivity of the marine diatom Phaeodactylum tricornutum grown on palm oil mill effluent

“…This implies that the availability of phosphorous in 30% v/v POME does not limit the growth of P. tricornutum under our experimental conditions. This finding was supported by a previous study who reported that a high amount of external nitrate increased the fucoxanthin productivity, growth rate, and biomass production of P. tricornutum and Odontella aurita cultivated on a modified f/2 and a modified L1 medium, respectively (Xia et al 2013;McClure et al 2018). Furthermore, early nitrogen limitation reduced the fucoxanthin productivity of P. tricornutum cultivated on a modified f/2 medium (Alipanah et al 2015).…”

Genome engineering empowers the diatom Phaeodactylum tricornutum for biotechnology

“…Their highly structured mesoporous cell wall of amorphous silica can be incorporated directly into nanodevices or used for the bioencapsidation and delivery of molecules 7 . Furthermore, diatoms are coveted organisms in the pharmaceutical, cosmetic and food industries as a natural source of polyunsaturated fatty acids, pigments and antioxidants [8][9][10][11] . Diatoms can also be used as expression systems for recombinant proteins or biomaterials 12,13 .…”

Odontella aurita-enriched diet prevents high fat diet-induced liver insulin resistance

“…Indeed, OA contains EPA and other components such as anti-oxidants, especially fucoxanthin known for its beneficial effects (Maeda et al 2009, Xia et al 2013, Tan & Hou 2014. Indeed, fucoxanthin has been described as having anti-obesity, anti-diabetic and anti-inflammatory effects (Maeda et al 2009, Tan & Hou 2014.…”