Cloning and Characterization of miRNAs and Their Targets, Including a Novel miRNA-Targeted NBS–LRR Protein Class Gene in Apple (Golden Delicious)

Ma, Chao; Lu, You; Bai, Songling; Zhang, Wennan; Duan, Xiaoqun; Meng, Dong; Wang, Zhigang; Wang, Aide; Zhou, Zhongshi; Li, Tianzhong

doi:10.1093/mp/sst101

Cited by 79 publications

(59 citation statements)

References 36 publications

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“…In another study using bioinformatic analysis and RNA library sequencing approaches, 146 miRNAs were identified from M. domestica (cv. Golden Delicious) of which 135 were conserved and 11 were novel miRNAs (Ma et al, 2014). This study showed that novel apple miRNA, Md-miRLn11, regulates the expression of a NBS–LRR protein during pathogen infection leading to resistance against the plant pathogenic bacterium ( Alternaria alternata f.sp.…”

Section: Functional Roles Of Mirnasmentioning

confidence: 99%

“…This study showed that novel apple miRNA, Md-miRLn11, regulates the expression of a NBS–LRR protein during pathogen infection leading to resistance against the plant pathogenic bacterium ( Alternaria alternata f.sp. mali; Ma et al, 2014). Recently, it was reported that four apple miRNAs (miR169a, miR160e, miR167b–g, and miR168a,b) are potentially involved in resistance to fire blight, a contagious bacterial disease caused by Erwinia amylovora by targeting stress response proteins (Kaja et al, 2015).…”

Section: Functional Roles Of Mirnasmentioning

confidence: 99%

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Functional Roles of microRNAs in Agronomically Important Plants—Potential as Targets for Crop Improvement and Protection

et al. 2017

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MicroRNAs (miRNAs) are a class of small non-coding RNAs that have recently emerged as important regulators of gene expression, mainly through cleavage and/or translation inhibition of the target mRNAs during or after transcription. miRNAs play important roles by regulating a multitude of biological processes in plants which include maintenance of genome integrity, development, metabolism, and adaptive responses toward environmental stresses. The increasing population of the world and their food demands requires focused efforts for the improvement of crop plants to ensure sustainable food production. Manipulation of mRNA transcript abundance via miRNA control provides a unique strategy for modulating differential plant gene expression and miRNAs are thus emerging as the next generation targets for genetic engineering for improvement of the agronomic properties of crops. However, a deeper understanding of its potential and the mechanisms involved will facilitate the design of suitable strategies to obtain the desirable traits with minimum trade-offs in the modified crops. In this regard, this review highlights the diverse roles of conserved and newly identified miRNAs in various food and industrial crops and recent advances made in the uses of miRNAs to improve plants of agronomically importance so as to significantly enhance crop yields and increase tolerance to various environmental stress agents of biotic-or abiotic origin.

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Section: Functional Roles Of Mirnasmentioning

confidence: 99%

Section: Functional Roles Of Mirnasmentioning

confidence: 99%

Functional Roles of microRNAs in Agronomically Important Plants—Potential as Targets for Crop Improvement and Protection

et al. 2017

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“…PbPTB3 was inserted into the PFGC5941 vector (Kerschen et al, 2004;Ma et al, 2014) between the SUC2 promoter and the mCherry gene.…”

Section: Vector Constructionmentioning

confidence: 99%

PbWoxT1 mRNA from pear (Pyrus betulaefolia) undergoes long‐distance transport assisted by a polypyrimidine tract binding protein

et al. 2015

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SummaryLittle is known about the mechanisms by which mRNAs are transported over long distances in the phloem between the rootstock and the scion in grafted woody plants.We identified an mRNA in the pear variety 'Du Li' (Pyrus betulaefolia) that was shown to be transportable in the phloem. It contains a WUSCHEL-RELATED HOMEOBOX (WOX) domain and was therefore named Wox Transport 1 (PbWoxT1).A 548-bp fragment of PbWoxT1 is critical in long-distance transport. PbWoxT1 is rich in CUCU polypyrimidine domains and its mRNAs interact with a polypyrimidine tract binding protein, PbPTB3. Furthermore, the expression of PbWoxT1 significantly increased in the stems of wild-type (WT) tobacco grafted onto the rootstocks of PbWoxT1 or PbPTB3 co-overexpressing lines, but this was not the case in WT plants grafted onto PbWoxT1 overexpressing rootstocks, suggesting that PbPTB3 mediates PbWoxT1 mRNA long-distance transport.We provide novel information that adds a new mechanism with which to explain the noncell-autonomous manner of WOX gene function, which enriches our understanding of how WOX genes work in fruit trees and other species.

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“…Taking advantage of small RNA deep sequencing data, they found 33 conserved and 42 apple-specific miRNAs. Ma et al (2013) further extended the list of apple miRNAs by 11 novel sequences. Altogether, there are 206 apple miRNAs deposited in miRBase release 20.…”

Section: Introductionmentioning

confidence: 99%

Identification of apple miRNAs and their potential role in fire blight resistance

Kaja

Szcześniak

Jensen

et al. 2014

Tree Genetics & Genomes

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MicroRNAs (miRNAs) are key players in multiple biological processes; therefore, analysis and characterization of these small regulatory RNAs is a critical step toward a better understanding of animal and plant biology. In apple (Malus domestica), 200 microRNAs are known, which most probably represent only a fraction of miRNAome diversity. As a result, more effort is required to better annotate miRNAs and their functions in this economically important species. We performed deep sequencing of 12 small RNA libraries obtained for fire blight-resistant and fire blight-sensitive trees. In the sequencing results, we identified 116 novel microRNAs and confirmed a majority of previously reported apple miRNAs. We then experimentally verified selected candidates with RT-PCR and stem-loop quantitative PCR (qPCR) and performed differential expression analysis. Finally, we identified and characterized putative targets of all known apple miRNAs. The gene ontology (GO) enrichment analysis suggests prominent roles of miRNAs in response to stresses, including pathogen infection. In this study, we identified 116 new and confirmed the expression of 143 already known miRNAs. Moreover, our data suggests that apple microRNAs might be considered as regulators and markers of fire blight resistance. The analyses we performed allowed us to define four apple miRNAs potentially involved in fire blight resistance in apple trees: mdm-miR169a, mdm-miR160e, mdm-miR167b-g, and mdm-miR168a,b. These miRNAs are known to be involved in response to stresses across other plant species, usually by targeting stress response proteins. The relatively low number of candidates may result from the high variance of biological replicates and the fact that stress response miRNAs are usually induced by the stress factors and frequently expressed at a low level, or not expressed at all, in normal conditions. The results of our studies are freely available in an online database at

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Cloning and Characterization of miRNAs and Their Targets, Including a Novel miRNA-Targeted NBS–LRR Protein Class Gene in Apple (Golden Delicious)

Cited by 79 publications

References 36 publications

Functional Roles of microRNAs in Agronomically Important Plants—Potential as Targets for Crop Improvement and Protection

Functional Roles of microRNAs in Agronomically Important Plants—Potential as Targets for Crop Improvement and Protection

PbWoxT1 mRNA from pear (Pyrus betulaefolia) undergoes long‐distance transport assisted by a polypyrimidine tract binding protein

Identification of apple miRNAs and their potential role in fire blight resistance

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