Timing it right: Precise ON/OFF switches for Rho1 and Cdc42 GTPases in cytokinesis

Balasubramanian, Mohan K.; Tao, Evelyn Yaqiong

doi:10.1083/jcb.201306152

Cited by 3 publications

(3 citation statements)

References 22 publications

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“…Understanding the temporal and very dynamic assembly of the cytokinetic apparatus in yeast and other organisms is technically demanding and far from complete (Balasubramanian and Tao, 2013;Pollard, 2010). One of the unresolved issues concerns the existence and composition of a central element of cytokinesis that is proposed to link and coordinate AMR contraction with septum synthesis in yeast or with extracellular matrix remodeling in the case of animal cells (Bi and Park, 2012;Onishi et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Stepwise and cooperative assembly of a cytokinetic core complex in yeast Saccharomyces cerevisiae

Tian

Johnsson

2014

Journal of Cell Science

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Actomyosin ring (AMR) contraction and the synthesis of an extracellular septum are interdependent pathways that mediate cytokinesis in the yeast Saccharomyces cerevisiae and other eukaryotes. How these interdependent pathways are physically connected is central for understanding cytokinesis. The yeast IQGAP (Iqg1p) belongs to the conserved AMR. The F-BAR-domaincontaining protein Hof1p is a member of a complex that stimulates cell wall synthesis. We report here on the stepwise formation of a physical connection between both proteins. The C-terminal IQrepeats of Iqg1p first bind to the essential myosin light chain before both proteins assemble with Hof1p into the Mlc1p-Iqg1p-Hof1p (MIH) bridge. Mutations in Iqg1p that disrupt the MIH complex alter Hof1p targeting to the AMR and impair AMR contraction. Epistasis analyses of two IQG1 alleles that are incompatible with formation of the MIH complex support the existence and functional significance of a large cytokinetic core complex. We propose that the MIH complex acts as hinge between the AMR and the proteins involved in cell wall synthesis and membrane attachment.

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Section: Introductionmentioning

confidence: 99%

Stepwise and cooperative assembly of a cytokinetic core complex in yeast Saccharomyces cerevisiae

Tian

Johnsson

2014

Journal of Cell Science

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“…Budding yeast divide where a bud grows from the mother cell ( Meitinger and Palani, 2016 ; Bhavsar-Jog and Bi, 2017 ). Very early in the cell cycle, polarity signals involving the GTPase Cdc42 establish the bud site ( Balasubramanian and Tao, 2013 ). Secondarily, the contractile ring assembles from septins, myosin-II, formins, and actin filaments in the neck between the mother and bud.…”

Section: Question 2: How Do Cells Position the Contractile Ring?mentioning

confidence: 99%

“…An IQGAP anchors the myosin-II after Bni5 dissociates ( Fang et al, 2010 ). Thereafter, the GTPase Rho1 activates the formin Bni1 to assemble actin filaments de novo ( Tolliday et al, 2002 ; Balasubramanian and Tao, 2013 ). In spite of the fact that these contractile rings form in a preexisting furrow, the molecular interactions are closely related to those in cells where the contractile ring initiates the cytokinetic furrow.…”

Section: Question 3: How Do Cells Assemble Cytokinetic Contractile Rimentioning

confidence: 99%

Nine unanswered questions about cytokinesis

Pollard

2017

Journal of Cell Biology

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Analysis of Rho-GTPase Activity During Budding Yeast Cytokinesis

Onishi

Pringle

2016

Methods in Molecular Biology

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Rho-type small GTPases are involved in cytokinesis in various organisms, but their precise roles and regulation remain unclear. Rho proteins function as molecular switches by cycling between the active GTP-bound and inactive GDP-bound states; the GTP-bound proteins in turn interact with their downstream effectors to transmit the signal. Biochemical assays using Rho-binding domains of effector proteins have been used to specifically pull down GTP-bound Rho proteins from cell extracts. Here, we describe the application of such a method in combination with cell-cycle synchronization in the budding yeast Saccharomyces cerevisiae; this approach allows dissection of the activity of Rho1 at different stages of cytokinesis. We also present data showing the importance of caution in interpreting such biochemical data and of comparing to the results obtained with other approaches where possible. The principle of this protocol is also applicable to analyses of other Rho-type GTPases and cell-cycle events.

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Timing it right: Precise ON/OFF switches for Rho1 and Cdc42 GTPases in cytokinesis

Cited by 3 publications

References 22 publications

Stepwise and cooperative assembly of a cytokinetic core complex in yeast Saccharomyces cerevisiae

Stepwise and cooperative assembly of a cytokinetic core complex in yeast Saccharomyces cerevisiae

Nine unanswered questions about cytokinesis

Analysis of Rho-GTPase Activity During Budding Yeast Cytokinesis

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