The Expression and Localization of LRF in the Female Reproductive Tract of Cycling Mice Throughout the Estrous Cycle

Abstract

In this article, the expression patterns of LRF in the mouse oviduct, uterus, and ovary were checked during estrous cycle. The expression of LRF mRNA and protein were remarkably changed in the ovary, oviduct, and uterus at four phases. LRF immunostaining was detected in the follicle from primary to antral follicle, luminal and glandular epithelial cells of the uterus, and cilia of the ciliated cells of the oviduct at all phase. Our findings suggested that LRF may be related to the processes of development and maturation of oocyte, gamete transport, and the development of early embryo.

Hormone regulates endometrial function via cooperation of endoplasmic reticulum stress and mTOR‐autophagy

“…Our previous studies have reported that 78‐kDa glucose‐regulated protein (GRP78), which is the main regulator of UPR, is significantly increased in implantation site on day 5 in mice (Lin et al, ). ERS‐response‐regulated protein, CREBZF, CREB3 (Luman), and CREBRF (LRF), are also up‐regulated in implantation site in mice (Lan et al, ; Li et al, ; Lin et al, ; Yang, Jin, Lin, et al, ; Yang, Jin, Martyn, et al, ). Therefore, our limited knowledge leads us to speculate that ER stress is involved in regulating embryo implantation in mice.…”

Reference gene selection for real-time quantitative PCR analysis on ovarian cryopreservation by vitrification in mice

“…Extraction of total RNA and cDNA synthesis TRIZOL reagent (Invitrogen Co., Carlsbad, CA, USA) was used for RNA isolation from 32 tissue samples, and the process was performed as previously described [7,8]. RNA samples were dissolved with RNase-free DNaseI (Promega, Fitchburg, WI, USA) before reverse transcription.…”

“…The four stages of the estrus cycle were tracked by vaginal smear according to previous studies [7,8], and mice in dioestrus were used for this study.…”

Distribuição espacial de transcritos no trato reprodutivo e sua relação com o ambiente endócrino periovulatório no início do diestro de bovinos de corte

“…As concentrações de P4 plasmática também foram mais altas na primeira ramificação venosa da região anterior do corno uterino ipsolateral ao CL do que na última ramificação da região posterior do mesmo corno, no corno contralateral e ainda da jugular. Também foi realizada a ligadura e secção da veia tubária nas ovelhas e resultando nos mesmos encontrados em vacas de leite, e induzindo a acreditar que há transferência de P4 pela veia tubária em ruminantes (WEEMS et al, 1988(WEEMS et al, , 1989 Transferência por contracorrente no pedículo vascular do ovário eleva a concentração de hormônios esteroides no sangue durante o ciclo estral de porcas (STEFAFICZYK-KRZYMOWSKA et al, 1994 (LIN et al, 2012;YANG et al, 2013), jacarés , entre outros, principalmente ao longo do ciclo estral mas não relacionados a protocolos hormonais desenvolvidos para aumentar a fertilidade.…”

“…FAHEY; WIRA, 2012), camundongos (LIN et al, 2012;YANG et al, 2013), suínos (BUHI et al, 2003), jacarés entre outros. Trabalhos com oviduto ratificaram que a proximidade com o folículo dominante ou com o CL funcional altera a expressão gênica (BAUERSACHS et al, 2003), proteica e concentrações hormonais desse tecido (WIJAYAGUNAWARDANE et al, 1998) (CLEMENTE et al, 2009;FORDE et al, 2011).…”