Cytological Evaluation and REBA HPV-ID HPV Testing of Newly Developed Liquid-Based Cytology, EASYPREP: Comparison with SurePath

Abstract

BackgroundThe objective of this study was to evaluate a newly-developed EASYPREP liquid-based cytology method in cervicovaginal specimens and compare it with SurePath.MethodsCervicovaginal specimens were prospectively collected from 1,000 patients with EASYPREP and SurePath. The specimens were first collected by brushing for SurePath and second for EASYPREP. The specimens of both methods were diagnosed according to the Bethesda System. Additionally, we performed to REBA HPV-ID genotyping and sequencing analysis for human papillomavirus (HPV) on 249 specimens.ResultsEASYPREP and SurePath showed even distribution of cells and were equal in cellularity and staining quality. The diagnostic agreement between the two methods was 96.5%. Based on the standard of SurePath, the sensitivity, specificity, positive predictive value, and negative predictive value of EASYPREP were 90.7%, 99.2%, 94.8%, and 98.5%, respectively. The positivity of REBA HPV-ID was 49.4% and 95.1% in normal and abnormal cytological samples, respectively. The result of REBA HPV-ID had high concordance with sequencing analysis.ConclusionsEASYPREP provided comparable results to SurePath in the diagnosis and staining quality of cytology examinations and in HPV testing with REBA HPV-ID. EASYPREP could be another LBC method choice for the cervicovaginal specimens. Additionally, REBA HPV-ID may be a useful method for HPV genotyping.

Comparison of EASYPREP® and SurePath® in thyroid fine‐needle aspiration

“…The minimum number of cases per run was 4 in both EP and SP. 11 In the present study, overall, EP showed results comparable to those of SP. The processing speed was equal between the two methods as 40 min for 48 cases.…”

“…SurePath V R (BD Diagnostics-TriPath Imaging, Burlington, NC, USA) (SP), an alternative FDA approved LBP, otherwise adopts a centrifugation method for cell enrichment in a manual process. 11 This system is innovative in that it is automated to avoid contamination during manual processes and to reduce labor time for batch sample preparation. 6,7 Over the last 25 years, the diagnostic utility of TP and SP has been validated in many studies using gynecologic samples.…”

“…As a result, it leaves more background materials and inflammatory cells so that it can preserve valuable background findings. 11 However, no data regarding the diagnostic utility of these techniques for thyroid samples have yet been published. [6][7][8][9] With respect to thyroid samples, LBP is gaining acceptance as a comparable or supportive test to CS preparation, although more integrated research is warranted.…”

“…1,[6][7][8]10 In 2012, a new automated LBP system, known as EASYPREP V R (EP) (YD Diagnostics Corp., Seoul, Korea), was developed, which includes a centrifugation method for cell enrichment. 11 This system is innovative in that it is automated to avoid contamination during manual processes and to reduce labor time for batch sample preparation. Recently, a comparative analysis between EP and SP using gynecologic samples showed that the diagnostic utility of EP was comparable to that of SP with successful application of ancillary molecular study.…”

Evaluation of nuclear chromatin using grayscale intensity and thresholded percentage area in liquid‐based cervical cytology

“…After obtaining Internal Review Board approval with a waiver of informed consent, an institutional cytopathology database was searched to identify the cases interpreted as HGSIL on liquid‐based cytology test at the Department of Pathology, Medical Center of Eulji University from January 1, 2015 to December 31, 2017. We reviewed a total of 22 selected cases of HGSIL diagnosed by density gradient‐based Easyprep liquid‐based cytological examination. The cytological diagnosis of HGSIL was confirmed by cervical biopsy or conization in all cases.…”