Phospholipids in sera of horses with summer eczema: Lipid analysis of the autoserum preparation used in therapy

Hallamaa, Raija; Batchu, Krishna Chaithanya; Tallberg, T

doi:10.1111/evj.12135

Cited by 6 publications

(11 citation statements)

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“…The additional aim was to assess whether profiles of the detected phospholipids change according to clinical status when horses were treated with an autogenous serum preparation. In our previous study [ 35 ], we found that autoserum preparations made from the sera of healthy horses and from horses with allergic eczema showed significantly different phospholipid profiles. Additionally, lipid concentrations displayed significant relationship with the severity of clinical signs.…”

Section: Discussionmentioning

confidence: 99%

“…Traces of PA were found in all samples and as an inner leaflet phospholipid, it is not normally detected from the horse serum [ 14 ]. Presence of PA is probably related to blood cells that remained when the samples were harvested without centrifugation [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

“…The lower concentration of PC and SM detected in the sera of affected horses was an unexpected finding, since our earlier study demonstrated that autoserum preparations made from the sera of horses with allergic dermatitis showed significantly higher concentrations of PC and SM than the preparations made from the sera of healthy horses [ 35 ]. This contradictory result may be associated with the hydrophobic/hydrophilic interactions of the lipid particles.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore there is a need for comprehensive understanding of this harmful disease. In our previous study [ 35 ], we analysed phospholipid contents of autoserum preparations that have been recently used in the therapy of insect hypersensitivity in horses. The idea is to collect remnant lipid particles in these preparations after serial washings [ 35 ].…”

Section: Introductionmentioning

confidence: 99%

“…In our previous study [ 35 ], we analysed phospholipid contents of autoserum preparations that have been recently used in the therapy of insect hypersensitivity in horses. The idea is to collect remnant lipid particles in these preparations after serial washings [ 35 ]. Concentrations of phosphatidylcholine (PC) and sphingomyelin (SM) were significantly more abundant in the preparations made from the sera of the affected than from healthy horses and the amounts of these phospholipids showed significant associations with the severity of prevailing clinical status [ 35 ].…”

Section: Introductionmentioning

confidence: 99%

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Phospholipid analysis in sera of horses with allergic dermatitis and in matched healthy controls

Hallamaa

Batchu

2016

Lipids Health Dis

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BackgroundLipids have become an important target for searching new biomarkers typical of different autoimmune and allergic diseases. The most common allergic dermatitis of the horse is related to stings of insects and is known as insect bite hypersensitivity (IBH) or summer eczema, referring to its recurrence during the summer months. This intense pruritus has certain similarities with atopic dermatitis of humans. The treatment of IBH is difficult and therefore new strategies for therapy are needed. Autoserum therapy based on the use of serum phospholipids has recently been introduced for horses. So far, serum lipids relating to these allergic disorders have been poorly determined. The main aim of this study was to analyse phospholipid profiles in the sera of horses with allergic dermatitis and in their healthy controls and to further assess whether these lipid profiles change according to the clinical status after therapy.MethodsSera were collected from 10 horses with allergic dermatitis and from 10 matched healthy controls both before and 4 weeks after the therapy of the affected horses. Eczema horses were treated with an autogenous preparation made from a horse’s own serum and used for oral medication. Samples were analysed for their phospholipid content by liquid chromatography coupled to a triple-quadrupole mass spectrometer (LC-MS). Data of phospholipid concentrations between the groups and over the time were analysed by using the Friedman test. Correlations between the change of concentrations and the clinical status were assessed by Spearman’s rank correlation test.ResultsThe major phospholipid classes detected were phosphatidylcholine (PC), sphingomyelin (SM), phosphatidylinositol (PI) and phosphatidylethanolamine (PE). Eczema horses had significantly lower total concentrations of PC (p < 0.0001) and SM (p = 0.0115) than their healthy controls. After a 4-week therapy, no significant differences were found between the groups. Changes in SM concentrations correlated significantly with alterations in clinical signs (p = 0.0047).ConclusionsHorses with allergic dermatitis have an altered phospholipid profile in their sera as compared with healthy horses and these profiles seem to change according to their clinical status. Sphingomyelin seems to have an active role in the course of equine insect bite hypersensitivity.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%