GLABROUS INFLORESCENCE STEMS regulates trichome branching by genetically interacting with SIM in Arabidopsis

Abstract

Arabidopsis trichomes are large branched single cells that protrude from the epidermis. The first morphological indication of trichome development is an increase in nuclear content resulting from an initial cycle of endoreduplication. Our previous study has shown that the C2H2 zinc finger protein GLABROUS INFLORESCENCE STEMS (GIS) is required for trichome initiation in the inflorescence organ and for trichome branching in response to gibberellic acid signaling, although GIS gene does not play a direct role in regulating trichome cell division. Here, we describe a novel role of GIS, controlling trichome cell division indirectly by interacting genetically with a key endoreduplication regulator SIAMESE (SIM). Our molecular and genetic studies have shown that GIS might indireclty control cell division and trichome branching by acting downstream of SIM. A loss of function mutation of SIM signficantly reduced the expression of GIS. Futhermore, the overexpression of GIS rescued the trichome cluster cell phenotypes of sim mutant. The gain or loss of function of GIS had no significant effect on the expression of SIM. These results suggest that GIS may play an indirect role in regulating trichome cell division by genetically interacting with SIM.

The Arabidopsis Gene zinc finger protein 3(ZFP3) Is Involved in Salt Stress and Osmotic Stress Response

“…It was reported that they participate in various biological processes including signal transduction, DNA/RNA binding, morphogenesis, transcription regulation, abiotic and biotic response [4,16–19,41]. Our previous work also have demonstrated that C2H2-type ZFPs GIS, GIS2, GIS3, ZFP8, ZFP5 and ZFP6 are all C 2 H 2 -type ZFPs control the trichome initiation by integrating GA and Cytokinin signals [18–21,23,42,43]. AtZFP1 was reported to plays a role in downstream of photomorphogenic activation [44].…”

GLABROUS INFLORESCENCE STEMS3 (GIS3) regulates trichome initiation and development in Arabidopsis

“…Mutants from F 2 and F 3 populations were identified by double selection on both kanamycin (50 mg l −1 ) and Basta (35 mg l −1 ). PCR was performed for further confirmation to verify homozygous zfp5 gis3 , zfp6 gis3 double mutants and the zfp5 zfp6 gis3 triple mutant (Sun et al ., ).…”

MADS-box Transcription Factor OsMADS25 Regulates Root Development through Affection of Nitrate Accumulation in Rice

“…The results were analyzed by means of ANOVA for significance by IBM SPSS Statistics 21. Student’s t-test was analyzed to evaluate the significant difference between treatments at the probability at either 5% (P<0.05 with significant level *) or at 1% (P<0.01 with significant level **) as we previously described [ 50 , 51 ].…”