MiR-335, an Adipogenesis-Related MicroRNA, is Involved in Adipose Tissue Inflammation

Zhu, Lu; Chen, Ling; Shi, Chunmei; Xu, Gao; Xu, Li; Zhu, Lijing; Guo, Xirong; Ni, Yuhui; Cui, Yan; Ji, Chenbo

doi:10.1007/s12013-013-9708-3

Cited by 75 publications

(64 citation statements)

References 36 publications

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“…Stouthard et al reported that increased glucose transport was observed in 3T3-L1 adipocytes following IL-6 treatment [29] and that IL-6 may lead to mitochondria dysfunction by inducing oxidative stress [30]. In addition, IL-6 has been shown to regulate adipocyte-related microRNA expression, such as the expression of miR-335 [16]. In this study, we explored the most likely effect of IL-6 on miR-378 during adipocyte differentiation, and we observed that IL-6 induced miR-378 expression in human mature adipocytes.…”

Section: Discussionmentioning

confidence: 99%

“…Briefly, samples were incubated at 95 °C for 10 min for an initial denaturation, followed by 40 PCR cycles of incubation at 95 °C for 15 s and then 60 °C for 1 min. miRNA expression was normalized to snRNA U6 and miR-103 [16]. Primer identification numbers are 000567 for hsamiR- 378, 000439 for miR-103, and 001973 for snRU6 (ABI, Foster City, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

“…Recently, a growing body of evidence suggests that the deregulation of miRNAs is closely associated with obesity-related metabolic syndrome, including type 2 diabetes mellitus (T2DM) [13,14]. Furthermore, some miRNAs regulated by adiponectin were identified as novel targets for controlling adipose tissue inflammation [15,16]. …”

Section: Introductionmentioning

confidence: 99%

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Insight into the Effects of Adipose Tissue Inflammation Factors on miR-378 Expression and the Underlying Mechanism

Jiang¹,

Xue²,

Fu³

et al. 2014

Cell Physiol Biochem

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Background/Aims: Obesity and the related metabolic syndrome have emerged as major public health issues in modern society. miRNAs have been shown to play key roles in regulating obesity-related metabolic syndrome, and some miRNAs regulated by adiponectin were identified as novel targets for controlling adipose tissue inflammation. miR-378 is a candidate target that was shown to be involved in adipose differentiation, mitochondrial metabolism and systemic energy homeostasis. However, little is known about the regulatory mechanisms of miR-378 expression. To better understand the physiological role of miR-378 in obesity and metabolic syndrome, it is crucial that we understand the regulation of miR-378 gene expression in human adipocytes. Methods: In this study, we investigated the effects of adipokines and inflammatory cytokines on miR-378 expression using Real-time PCR and the potential regulatory mechanisms using luciferase reporter assays and electrophoretic mobility shift assay (EMSA). Results: We found that adipokines and cytokines upregulated miR-378 expression primarily through SREBP and C/EBP binding sites in the miR-378 promoter region. Conclusion: Our findings showed that adipokines induced miR-378 expression and revealed the most likely mechanism of adipokine-induced miR-378 dysregulation in human adipocytes. miRNAs have been shown to function in regulating obesity-related metabolic syndrome, and miR-378 may be a novel target for controlling adipose tissue inflammation. This study offers a theoretical basis for understanding systemic adipose tissue inflammation and may provide new strategies for clinical treatment.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Insight into the Effects of Adipose Tissue Inflammation Factors on miR-378 Expression and the Underlying Mechanism

Jiang¹,

Xue²,

Fu³

et al. 2014

Cell Physiol Biochem

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“…The role of miRNAs in inflammatory response in many diseases has been reviewed (49). Among miRNAs associated with inflammation, miR-155 is overexpressed in adipose tissue from obese patients (32), and miR-335 expression is stimulated by leptin, resistin, TNF-␣, and IL-6 in human adipocytes (91). Investigations of miR-132 expression in human primary preadipocytes and in differentiated adipocytes established a relationship between overexpression of miR-132 and induction of nuclear factorkappa B translocation, acetylation of p65, and production of IL-8 and monocyte chemoattractant protein-1 (MCP-1).…”

Section: Mirna-mediated Regulation Of Insulin Sensitivity In Adipose mentioning

confidence: 99%

Biological roles of microRNAs in the control of insulin secretion and action

Caldérari

Diawara

Garaud

et al. 2017

Physiological Genomics

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microRNAs (miRNAs) are intracellular and circulating molecular components contributing to genome expression control through binding mRNA targets, which generally results in downregulated mRNA expression. One miRNA can target several mRNAs, and one transcript can be targeted by several miRNAs, resulting in complex fine-tuning of regulation of gene networks and signaling pathways. miRNAs regulate metabolism, adipocyte differentiation, pancreatic development, β-cell mass, insulin biosynthesis, secretion, and signaling, and their role in diabetes and obesity is emerging. Their pathophysiological effects are essentially dependent on cellular coexpression with their mRNA targets, which can show tissue-specific transcriptional responses to disease conditions and environmental challenges. Current knowledge of miRNA biology and their impact on the pathogenesis of diabetes and obesity is based on experimental data documenting miRNA expression generally in single tissue types that can be correlated with expression of target mRNAs to integrate miRNA in functional pathways and gene networks. Here we present results from the most significant studies dealing with miRNA function in liver, fat, skeletal muscle, and endocrine pancreas and their implication in diabetes and obesity

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“…Regulation of the transcription of miR-335 by these adipokines is independent of the native gene (the gene MEST). Accordingly, the occurrence of complications of obesity caused by the function of miR-335 is dependent on his own mechanisms of transcription regulated by the presence of the adipokines (Zhu et al, 2014). Bielicki, et al (2014) investigated the relationship between polymorphisms of selected genes possibly associated with coronary heart disease with a prevalence of obstructive sleep apnea (OSA).…”

Section: Adiponectin -The Adipokine Of Special Rolementioning

confidence: 99%

The genetic basis of obesity complications

Skrypnik

Suliburska

Skrypnik

et al. 2017

Acta Sci Pol Technol Aliment

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Intensive research is currently being performed into the genetic background of excess body mass complications such as diabetes, cardiovascular disorders, especially atherosclerosis and coronary heart disease. Chronic inflammation is an important process in the pathogenesis of obesity, wherein there is an aberrant expression of genes encoding adipokines. Visceral tissue is characterized by a higher expression and secretion of interleukin-8, interleukin-1ß and plasminogen activator inhibitor 1 in the subcutaneous tissue secretion of leptin prevails. An important complication of obesity is obstructive sleep apnea, often observed in PraderWilli syndrome. The genetic background of sleep apnea may be a polymorphism of the SREBF1 gene. The consequence of excess body mass is metabolic syndrome, which may be related to the occurrence of the rs926198 variant of gene encoding caveolin-1. The genes of transcription factor TCF7L2 and PPAR-γ2 take part in the pathogenesis of diabetes development. It has been demonstrated that oncogenes FOS, FOSB, and JUN may be co-responsible not only for obesity but also for osteoporosis and colorectal cancer. It has been shown that weight loss causes a modification in the expression of about 100 genes involvedt in the production of substances such as cytokines and other responsible for chronic inflammation in obesity. In future studies on the complications of obesity, such scientific disciplines as proteomics, peptidomics, metabolomics and transcriptomics should be used. The aim of this study is to present the current state of knowledge about the genetic basis of obesity complications.

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MiR-335, an Adipogenesis-Related MicroRNA, is Involved in Adipose Tissue Inflammation

Cited by 75 publications

References 36 publications

Insight into the Effects of Adipose Tissue Inflammation Factors on miR-378 Expression and the Underlying Mechanism

Insight into the Effects of Adipose Tissue Inflammation Factors on miR-378 Expression and the Underlying Mechanism

Biological roles of microRNAs in the control of insulin secretion and action

The genetic basis of obesity complications

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