Enhanced beta-catenin expression and inflammation are associated with human ectopic tubal pregnancy

Li, Ping; Zhu, Weijie; Ma, Zheng-lai; Wang, Guang; Peng, Hui; Chen, Yao; Lee, Kenneth K.; Yang, Xuesong

doi:10.1093/humrep/det246

Cited by 22 publications

(16 citation statements)

References 37 publications

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“…We also determined that expression of WNT/ CTNNB1 signaling‐related genes in the oviduct was regulated by E 2 . Recent findings indicate that the expression of CTNNB1 is increased in the ampulla region of fallopian tubes of patients with diseases, such as salpingitis, and ectopic pregnancy (41). Together with our findings, these observations suggest that aberrant expression of CTNNB1 in the fallopian tubes could result in ectopic pregnancy.…”

Section: Discussionmentioning

confidence: 99%

Estrogen receptor α is required for oviductal transport of embryos

O’Neill

Zhang

et al. 2017

FASEB j.

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Newly fertilized embryos spend the first few days within the oviduct and are transported to the uterus, where they implant onto the uterine wall. An implantation of the embryo before reaching the uterus could result in ectopic pregnancy and lead to maternal death. Estrogen is necessary for embryo transport in mammals; however, the mechanism involved in estrogen-mediated cellular function within the oviduct remains unclear. In this study, we show in mouse models that ciliary length and beat frequency of the oviductal epithelial cells are regulated through estrogen receptor α (ESR1) but not estrogen receptor β (ESR2). Gene profiling indicated that transcripts in the WNT/β-catenin (WNT/CTNNB1) signaling pathway were regulated by estrogen in mouse oviduct, and inhibition of this pathway in a whole oviduct culture system resulted in a decreased embryo transport distance. However, selective ablation of CTNNB1 from the oviductal ciliated cells did not affect embryo transport, possibly because of a compensatory mechanism intact CTNNB1 in the adjacent secretory cells. In summary, we demonstrated that disruption of estrogen signaling in oviductal epithelial cells alters ciliary function and impairs embryo transport. Therefore, our findings may provide a better understanding of etiology of the ectopic pregnancy that is associated with alteration of estrogen signals.-Li, S., O'Neill, S. R. S., Zhang, Y., Holtzman, M. J., Takemaru, K.-I., Korach, K. S., Winuthayanon, W. Estrogen receptor α is required for oviductal transport of embryos.

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Section: Discussionmentioning

confidence: 99%

Estrogen receptor α is required for oviductal transport of embryos

O’Neill

Zhang

et al. 2017

FASEB j.

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“…Mainly localized to cell-cell junctions, β-catenin plays an important role in intercellular adhesion, cell polarity, and architecture by binding to E-cadherin [21, 22]. Compared with non-pregnant tubal tissues, immunohistochemistry demonstrated that β-catenin was strongly expressed at tubal implantation sites in EPs following IVF-ET [23]. It is possible that the downregulation of CXXC5 increases expression of β-catenin, leading to a higher risk of EP in DOR.…”

Section: Discussionmentioning

confidence: 99%

Increased incidence of ectopic pregnancy after in vitro fertilization in women with decreased ovarian reserve

et al. 2017

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The incidence of ectopic pregnancy after assisted reproductive technology is increased approximately 2.5–5-fold compared with natural conceptions.Strategies were used to decrease the incidence of ectopic pregnancy, but ectopic pregnancy still occurs. In the present study, women were selected with decreased ovarian reserve (defined as FSH > 10 IU/L) aged 20 to 38 years who underwent IVF-ET between 2009 and 2014. These 2,061 women were age-matched with an equal number of women with normal ovarian reserve (defined as FSH ≤ 10 IU/L). During cycles following fresh embryo transfer, 93 patients were diagnosed with ectopic pregnancy. The incidence of ectopic pregnancy in clinical pregnancies was significantly higher in the decreased ovarian reserve than in the normal ovarian reserve group (5.51% vs. 2.99%). After adjusting for confounding factors, the incidence of ectopic pregnancy was significantly associated with decreased ovarian reserve. Our results showed that decreased ovarian reserve is an independent risk factor for ectopic pregnancy after in vitro fertilization-embryo transfer.

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“…Previous studies by us and others have shown that SLIT2‐ROBO1 signalling is involved in placental angiogenesis . VEGFA is the most potent angiogenic factor and is regulated directly by HIF1α . R5 treatment inhibits HIF1α and VEGFA expression.…”

Section: Discussionmentioning

confidence: 94%

“…Antibodies were used against the following targets: CK‐7 (Maixin Group, ShenZhen, China), E‐cadherin (Becton Dickinson, San Jose, CA, USA), SLIT2 (Santa Cruz Inc., Santa Cruz, CA, USA), Robo1 (Abcam, Cambridge, MA, USA), AP‐2α (Santa Cruz) and phospho‐histone H3 (PH3) (Santa Cruz). Immunohistochemistry and immunofluorescent staining and semiquantitative analysis by immunohistochemical reactive scores (IRSs) were conducted as described previously . The immunofluorescent stainings were counted manually at 4′,6‐diamidino‐2‐phenylindole (DAPI) and PH3 immunostaining merged images.…”

Section: Methodsmentioning

confidence: 99%

Alterted SLIT2/ROBO1 signalling is linked to impaired placentation of missed and threatened miscarriage in early pregnancy

Shi

Shuai

et al. 2017

Histopathology

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Enhanced beta-catenin expression and inflammation are associated with human ectopic tubal pregnancy

Abstract: A better understanding of the underlying mechanisms leading to ectopic pregnancies may contribute to our knowledge of the pathogenesis of tubal disorders and infertility and to the prevention of tubal ectopic pregnancy.

Cited by 22 publications

References 37 publications

Estrogen receptor α is required for oviductal transport of embryos

Estrogen receptor α is required for oviductal transport of embryos

Increased incidence of ectopic pregnancy after in vitro fertilization in women with decreased ovarian reserve

Alterted SLIT2/ROBO1 signalling is linked to impaired placentation of missed and threatened miscarriage in early pregnancy

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