The expression patterns of bromelain and AcCYS1 correlate with blackheart resistance in pineapple fruits submitted to postharvest chilling stress

Raimbault, Astrid-Kim; Zuily-Fodil, Yasmine; Soler, Alain; Mora, Phillipe; Carvalho, Maria Helena Cruz de

doi:10.1016/j.jplph.2013.05.008

Cited by 14 publications

(6 citation statements)

References 26 publications

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“…Similar defense responses to pests and pathogens had been observed in other plants expressing CP genes [2,4,42,44,45]. Expression levels of AcCYS1 ( A. comusus cystatin 1, an endogenous inhibitor of bromelain) are directly related with the resistance to blackheart in pineapple fruits, which implies that CP takes part in the defense response [43]. Increase of CP activity can enhance the degradation of gelatin-based jellies in vitro or the processing of a class IV chitinase in planta [46].…”

Section: Resultsmentioning

confidence: 56%

“…Yamada et al also found that CP was responsible for the maturity and activation of vacuole hydrolytic enzymes, which were involved in the degradation of cellular components sequestered from the cytosol by autophagy [40]. Plant CPs also participate in response to adverse environmental stresses, and possess remarkable toxicity against pathogens [4,41,42,43]. The classic examples demonstrated that papain or papain-like enzymes in the latex of papaya and fig ( Ficus virgata ) inhibited the growth of lepidopteran larvae [41].…”

Section: Resultsmentioning

confidence: 99%

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Functional Properties of a Cysteine Proteinase from Pineapple Fruit with Improved Resistance to Fungal Pathogens in Arabidopsis thaliana

et al. 2014

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Abstract:In plant cells, many cysteine proteinases (CPs) are synthesized as precursors in the endoplasmic reticulum, and then are subject to post-translational modifications to form the active mature proteinases. They participate in various cellular and physiological functions. Here, AcCP2, a CP from pineapple fruit (Ananas comosus L.) belonging to the C1A subfamily is analyzed based on the molecular modeling and homology alignment. Transcripts of AcCP2 can be detected in the different parts of fruits (particularly outer sarcocarps), and gradually increased during fruit development until maturity. To analyze the substrate specificity of AcCP2, the recombinant protein was overexpressed and purified from Pichia pastoris. The precursor of purified AcCP2 can be processed to a 25 kDa active form after acid treatment (pH 4.3). Its optimum proteolytic activity to Bz-Phe-Val-Arg- OPEN ACCESSMolecules 2014, 19 2375 NH-Mec is at neutral pH. In addition, the overexpression of AcCP2 gene in Arabidopsis thaliana can improve the resistance to fungal pathogen of Botrytis cinerea. These data indicate that AcCP2 is a multifunctional proteinase, and its expression could cause fruit developmental characteristics of pineapple and resistance responses in transgenic Arabidopsis plants.

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Section: Resultsmentioning

confidence: 56%

Section: Resultsmentioning

confidence: 99%

Functional Properties of a Cysteine Proteinase from Pineapple Fruit with Improved Resistance to Fungal Pathogens in Arabidopsis thaliana

et al. 2014

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“…In the absence of bacterial cells, the sink was the proteolytic degradation of nisin. In general, fruits may contain various types of proteases, such as cucumisin in melons, actinidin in kiwi fruit, and bromelain in pineapples (Yamagata and others ; Katsaros and others ; Raimbault and others ). Although these proteases vary with their pattern of proteolysis, they could all lead to significant degradation of peptides such as nisin.…”

Section: Resultsmentioning

confidence: 99%

Emulsion Stabilized with Starch Octenyl Succinate Prolongs Nisin Activity Against Listeria Monocytogenes in a Cantaloupe Juice Model

Sarkar

Bhunia

Yao

2016

Journal of Food Science

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Food safety related to fresh and fresh-cut produce, such as cantaloupe, is a great challenge in the food industry. Various processing technologies, including the use of antimicrobial materials, have been applied to reduce pathogens. Nisin, an antimicrobial peptide, shows strong inhibitory activity against Listeria monocytogenes (LM); however, it suffers from quick depletion in foods. In this study, the protective effect of starch octenyl succinate (starch-OS) stabilized emulsion on nisin was evaluated in a cantaloupe juice model. Results showed that the proteolytic enzyme in cantaloupe juice caused nisin depletion, and the adsorption of nisin in emulsion led to considerable protection of antimicrobial activity. After 1, 3, and 6 d of incorporation with cantaloupe juice, the size of LM inhibitory ring were 7.64, 7.60, and 2.97 mm, respectively for emulsion formulations, whereas it reduced from 3.60 mm to a negligible level for nisin-alone. This study showed the potential of using carbohydrate colloidal systems to prolong the efficacy of antimicrobial materials.

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“…6d). Bromelains coordinating with bromelain inhibitors are supposed to play an important role in pineapple fruit ripening 41,42 . Bromelain inhibitor is posttranslationally inactivated during fruit ripening, leading to a significant increase in bromelain activity, thus enhancing tissue proteolysis, (Fig.…”

Section: Nature Geneticsmentioning

confidence: 99%

The bracteatus pineapple genome and domestication of clonally propagated crops

et al. 2019

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Merr.) is a fruit crop originated and domesticated in South America. According to Bertoni 2 , the genus name Ananas means 'excellent fruit' in the Guaraní language of Paraguay. Pineapple was domesticated >6,000 years ago with archaeobotanical remains dated 3,500 years ago in South America and distributed to Mesoamerica >2,500 years ago 3-5. Pineapple is clonally propagated using the leafy fruit crown, slips or suckers. Red pineapple (Ananas comosus var. bracteatus) was anciently cultivated for fiber, fruit juice and as a living hedge, and is now a pantropical ornamental 6,7. The bracteatus plant is conspicuous for its bright pink-to-red colored fruit. The name 'bracteatus' refers to its long bracts. The plant is vigorous with long leaves, coarse spines and abundant suckers. Plant fibers have been used in numerous applications that are beneficial to agriculture and the environment, partly

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The expression patterns of bromelain and AcCYS1 correlate with blackheart resistance in pineapple fruits submitted to postharvest chilling stress

Cited by 14 publications

References 26 publications

Functional Properties of a Cysteine Proteinase from Pineapple Fruit with Improved Resistance to Fungal Pathogens in Arabidopsis thaliana

Functional Properties of a Cysteine Proteinase from Pineapple Fruit with Improved Resistance to Fungal Pathogens in Arabidopsis thaliana

Emulsion Stabilized with Starch Octenyl Succinate Prolongs Nisin Activity Against Listeria Monocytogenes in a Cantaloupe Juice Model

The bracteatus pineapple genome and domestication of clonally propagated crops

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