Real-Time Monitoring of Pseudomonas Aeruginosa Concentration Using a Novel Electromagnetic Sensors Microfluidic Cell Structure

Blakey, Richard; Nakouti, Ismini; Korostynska, O.; Mason, Alex; Al-Shamma’a, Ahmed

doi:10.1109/tbme.2013.2268277

Cited by 21 publications

(13 citation statements)

References 41 publications

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“…Electromagnetic (EM) wave sensors offer a reliable, real-time, cost-effective methodological tool for in situ P. aeruginosa growth analysis. EM wave sensors mounted on a polymethyl-methacrylate microfluidic cell structure contributed to the early detection of biofilm growth upon excitation at microwave frequencies (416).…”

Section: Extending Beyond Commonplace Platformsmentioning

confidence: 99%

Options and Limitations in Clinical Investigation of Bacterial Biofilms

et al. 2018

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Bacteria can form single- and multispecies biofilms exhibiting diverse features based upon the microbial composition of their community and microenvironment. The study of bacterial biofilm development has received great interest in the past 20 years and is motivated by the elegant complexity characteristic of these multicellular communities and their role in infectious diseases. Biofilms can thrive on virtually any surface and can be beneficial or detrimental based upon the community's interplay and the surface. Advances in the understanding of structural and functional variations and the roles that biofilms play in disease and host-pathogen interactions have been addressed through comprehensive literature searches. In this review article, a synopsis of the methodological landscape of biofilm analysis is provided, including an evaluation of the current trends in methodological research. We deem this worthwhile because a keyword-oriented bibliographical search reveals that less than 5% of the biofilm literature is devoted to methodology. In this report, we (i) summarize current methodologies for biofilm characterization, monitoring, and quantification; (ii) discuss advances in the discovery of effective imaging and sensing tools and modalities; (iii) provide an overview of tailored animal models that assess features of biofilm infections; and (iv) make recommendations defining the most appropriate methodological tools for clinical settings.

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Section: Extending Beyond Commonplace Platformsmentioning

confidence: 99%

Options and Limitations in Clinical Investigation of Bacterial Biofilms

et al. 2018

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“…An interdigitated shaped pattern printed on FR4 substrate and operating at microwave frequencies was chosen for its versatile design that combines ease of manufacturing with desired functionality [55]. Gold was used as a metal material for both bottom layer, which acted as a ground plane, and top pattern to maintain chemical neutrality when the device is placed in contact with aqueous media with bacteria.…”

Section: Microwave Sensor Head Structurementioning

confidence: 99%

Detection of Pathogenic Bacteria in Aqueous Media: Assessing the Potential of Real-Time Electromagnetic Wave Sensing

Nakouti

Korostynska

Mason

et al. 2014

International Journal on Smart Sensing and Intelligent Systems

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This paper reports on the capabilities of a novel electromagnetic wave sensing method to detect and identify the presence of various pathogenic bacteria in aqueous media. In particular, the change in the electromagnetic wave signal in microwave frequency range is used as an indicator of bacteria presence. The assessment was conducted by recording reflected signal spectra when the sensor was in contact with deionised water, Escherichia coli, sterile nutrient broth and Pseudomonas aeruginosa solutions. The distinct feature of the proposed system is that the detection is performed in real time, without the need for additional sample processing or chemicals. This bacteria detection method would be of benefit in a broad range of applications, ranging from water quality monitoring in wastewater treatment facilities to safety assurance in healthcare and food industry.

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“…EM wave sensors operating at microwave frequencies are seeing an increasing interest for minimally-invasive [2,3] and non-invasive [4][5][6] medical purposes. The sensors can typically be characterised as requiring low power (<1 mW) while retaining a good level of penetration into a target material that they may assess properties beneath a surface, even to determine a blood lactate level through the skin of a subject [7].…”

Section: Introductionmentioning

confidence: 99%

A549 Cells Measurements with Optical, Impedance and Microwave Spectroscopy

2018

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Lung cancer, especially lung adenocarcinoma, is one of the main causes of death worldwide. This paper reports on the real-time detection methods of human lung adenocarcinoma A549 cells. The proposed approach is based on using optical, impedance and microwave spectroscopy in an attempt to develop an online tool that can be easily used for early cancer diagnostics and treatment monitoring not only in the hospital labs, but at point of care. Low power athermal electromagnetic waves as a sensing mechanism for early cancer detection showed a particular promise. The techniques were also applied to other cells, such as PC3, LL24, HLF, HACAT and DU145, to ensure selectivity of the sensors. Providing real-time information at point of care will be a significant leap on its own as it will dramatically increase screening capabilities.

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Real-Time Monitoring of Pseudomonas Aeruginosa Concentration Using a Novel Electromagnetic Sensors Microfluidic Cell Structure

Cited by 21 publications

References 41 publications

Options and Limitations in Clinical Investigation of Bacterial Biofilms

Options and Limitations in Clinical Investigation of Bacterial Biofilms

Detection of Pathogenic Bacteria in Aqueous Media: Assessing the Potential of Real-Time Electromagnetic Wave Sensing

A549 Cells Measurements with Optical, Impedance and Microwave Spectroscopy

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