Contribution of the 7β-hydroxysteroid dehydrogenase from Ruminococcus gnavus N53 to ursodeoxycholic acid formation in the human colon

Lee, Jayoung; Arai, Hisashi; Nakamura, Yusuke; Fukiya, Satoru; Wada, Masaaki; Yokota, Atsushi

doi:10.1194/jlr.m039834

Cited by 121 publications

(105 citation statements)

References 42 publications

Supporting

Mentioning

102

Contrasting

Order By: Relevance

“…43 Organisms from the family Erysipelotrichaceae have been linked with IBD, are consistently positively associated with high-fat diets and dyslipidemia, and may be modulated by changes in host cholesterol metabolites. 44 while some species of the genus Ruminococcus appear to be protective, 4546 a greater abundance of Ruminococcus obeum was observed in the faeces of rats with 1,2-dimethyl hydrazine-induced precancerous lesions. 47 …”

Section: Discussionmentioning

confidence: 96%

Race-dependent association of sulfidogenic bacteria with colorectal cancer

Yazıcı

Wolf

Kim

et al. 2017

Gut

165

128

View full text Add to dashboard Cite

Objective Colorectal cancer (CRC) incidence is higher in African Americans (AAs) compared with non-Hispanic whites (NHWs). A diet high in animal protein and fat is an environmental risk factor for CRC development. The intestinal microbiota is postulated to modulate the effects of diet in promoting or preventing CRC. Hydrogen sulfide, produced by autochthonous sulfidogenic bacteria, triggers proinflammatory pathways and hyperproliferation, and is genotoxic. We hypothesised that sulfidogenic bacterial abundance in colonic mucosa may be an environmental CRC risk factor that distinguishes AA and NHW. Design Colonic biopsies from uninvolved or healthy mucosa from CRC cases and tumour-free controls were collected prospectively from five medical centres in Chicago for association studies. Sulfidogenic bacterial abundance in uninvolved colonic mucosa of AA and NHW CRC cases was compared with normal mucosa of AA and NHW controls. In addition, 16S rDNA sequencing was performed in AA cases and controls. Correlations were examined among bacterial targets, race, disease status and dietary intake. Results AAs harboured a greater abundance of sulfidogenic bacteria compared with NHWs regardless of disease status. Bilophila wadsworthia-specific dsrA was more abundant in AA cases than controls. Linear discriminant analysis of 16S rRNA gene sequences revealed five sulfidogenic genera that were more abundant in AA cases. Fat and protein intake and daily servings of meat were significantly higher in AAs compared with NHWs, and multiple dietary components correlated with a higher abundance of sulfidogenic bacteria. Conclusions These results implicate sulfidogenic bacteria as a potential environmental risk factor contributing to CRC development in AAs.

show abstract

Section: Discussionmentioning

confidence: 96%

Race-dependent association of sulfidogenic bacteria with colorectal cancer

Yazıcı

Wolf

Kim

et al. 2017

Gut

165

128

View full text Add to dashboard Cite

show abstract

“…For examples, R. gnavus was positively associated with fatness traits in both fecal and cecum samples. R. gnavus plays a pivotal role in UDCA formation in the colon, which regarded as a supplement of the bile acid (Lee et al, 2013). A recent study found that the α-galactosidase 1 (Aga1) and α-galactosidase 2 (Aga2) which are two kinds of the glycoside hydrolase (GH) family from R. gnavus played an indispensable role in the degradation of dietary oligosaccharides and exerted a tremendous fascination on designing of galacto-oligosaccharide (GOS) prebiotics (Cervera-Tison et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Evaluating the Contribution of Gut Microbiota to the Variation of Porcine Fatness with the Cecum and Fecal Samples

Fang

Huang

et al. 2016

Front. Microbiol.

View full text Add to dashboard Cite

Microbial community in gastrointestinal tract participates in the development of the obesity as well as quite a few metabolic diseases in human. However, there are few studies about the relationship between gut microbiota and porcine fatness. Here, we used high-throughput sequencing to perform 16S rRNA gene analysis in 256 cecum luminal samples from Erhualian pigs and 244 stools from Bamaxiang pigs, and adopted a two-part model statistical method to evaluate the association of gut microbes with porcine fatness. As the results, we identified a total of 6 and 108 operational taxonomic units (OTUs), and 9 and 10 bacterial taxa which showed significant associations with fatness traits in the stool and cecum samples, respectively. Cross-validation analysis indicated that gut microbiome showed the largest effect on abdominal adipose by explaining 2.73% phenotypic variance of abdominal fat weight. Significantly more fatness-associated OTUs were identified in the cecum samples than that in the stools, suggesting that cecum luminal samples were better used for identification of fatness-associated microbes than stools. The fatness-associated OTUs were mainly annotated to Lachnospiraceae, Ruminococcaceae, Prevotella, Treponema, and Bacteroides. These microbes have been reported to produce short-chain fatty acids by fermenting dietary indigested polysaccharide and pectin. The short-chain fatty acids can regulate host body energy homeostasis, protect host from inflammation and inhibit fat mass development. Our findings suggested that the gut microbiome may be an important factor modulating fatness in pigs.

show abstract

“…In the colon, there were no B. acidifaciens detected in NOD mice and fewer R. gnavus. B. acidifaciens induces production of secretory IgA in the gut (Yanagibashi et al, 2013), while R. gnavus has been shown to make antiinflammatory bile acid (Lee et al, 2013). Despite the differences in microbial profiles, a causative role for the microbiota in T1D progression in NOD mice has not yet been established.…”

Section: Discussionmentioning

confidence: 99%

Prolonged antibiotic treatment induces a diabetogenic intestinal microbiome that accelerates diabetes in NOD mice

Brown

Godovannyi

Ma³

et al. 2015

The ISME Journal

143

110

View full text Add to dashboard Cite

Accumulating evidence supports that the intestinal microbiome is involved in Type 1 diabetes (T1D) pathogenesis through the gut-pancreas nexus. Our aim was to determine whether the intestinal microbiota in the non-obese diabetic (NOD) mouse model played a role in T1D through the gut. To examine the effect of the intestinal microbiota on T1D onset, we manipulated gut microbes by: (1) the fecal transplantation between non-obese diabetic (NOD) and resistant (NOR) mice and (2) the oral antibiotic and probiotic treatment of NOD mice. We monitored diabetes onset, quantified CD4+T cells in the Peyer's patches, profiled the microbiome and measured fecal short-chain fatty acids (SCFA). The gut microbiota from NOD mice harbored more pathobionts and fewer beneficial microbes in comparison with NOR mice. Fecal transplantation of NOD microbes induced insulitis in NOR hosts suggesting that the NOD microbiome is diabetogenic. Moreover, antibiotic exposure accelerated diabetes onset in NOD mice accompanied by increased T-helper type 1 (Th1) and reduced Th17 cells in the intestinal lymphoid tissues. The diabetogenic microbiome was characterized by a metagenome altered in several metabolic gene clusters. Furthermore, diabetes susceptibility correlated with reduced fecal SCFAs. In an attempt to correct the diabetogenic microbiome, we administered VLS#3 probiotics to NOD mice but found that VSL#3 colonized the intestine poorly and did not delay diabetes. We conclude that NOD mice harbor gut microbes that induce diabetes and that their diabetogenic microbiome can be amplified early in life through antibiotic exposure. Protective microbes like VSL#3 are insufficient to overcome the effects of a diabetogenic microbiome.

show abstract

Contribution of the 7β-hydroxysteroid dehydrogenase from Ruminococcus gnavus N53 to ursodeoxycholic acid formation in the human colon

Cited by 121 publications

References 42 publications

Race-dependent association of sulfidogenic bacteria with colorectal cancer

Race-dependent association of sulfidogenic bacteria with colorectal cancer

Evaluating the Contribution of Gut Microbiota to the Variation of Porcine Fatness with the Cecum and Fecal Samples

Prolonged antibiotic treatment induces a diabetogenic intestinal microbiome that accelerates diabetes in NOD mice

Contact Info

Product

Resources

About