Ultrastructure and molecular characterization of the microsporidium, Nosema chrysoperlae sp. nov., from the green lacewing, Chrysoperla carnea (Stephens) (Neuroptera: Chrysopidae) used for biological pest control

Bjørnson, S.; Steele, T.; Hu, Qiongyao; Ellis, B.; Saito, Taro

doi:10.1016/j.jip.2013.05.007

Cited by 6 publications

(3 citation statements)

References 25 publications

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“…Depending on the degree of infection in populations of natural enemies, changes in the duration of the juvenile phase, reduced survival, altered flight behavior and host search may occur. These symptoms were reported in C. flavipes (Simões et al 2012), Cotesia vertalis (Haliday) (Hymenoptera: Braconidae) (Kermani et al 2014), Macrocentrus grandii goidanich (Hymenoptera: Braconidae) (Andreadis 1982), Phytoseiulus persimilis (Acari: Phytoseiidae) (Bjørnson and Keddie 1999) and Chrysoperla carnea (Stephens) (Neuroptera: Chrysopidae) (Bjørnson et al 2013). Thus, it is characterized the need for detailed investigation of the interaction between infection by this microsporid in hosts D. saccharalis and pupal parasitoids, T. diatraeae and P. elaeisis, to verify the quality and improve the understanding of the real impact of this parasite in biology and in the search behavior of the host in the field.…”

Section: Resultsmentioning

confidence: 99%

Infection by the microsporidium of Clado Nosema/Vairimorpha in pupal parasitoids

Paes

Carvalho

Souza

et al. 2019

An. Acad. Bras. Ciênc.

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The sugarcane borer, Diatraea saccharalis is one of the hosts more used for parasitoid pupal multiplication in Brazil. The parasitoids pupal of Trichospilus diatraeae and Palmistichus elaeisis are generalist natural enemies with potential to suppress populations of diverse families of lepidopteran pests. The success in the utilization of these natural enemies in the field is directly related to the capacity of search of the host, this capacity might be affected by the presence of the pathogens. In this context, the aim of this essay was to detect the presence of intracellular parasites of Phylum Microsporidia. These pathogens may cause morphological and behavioral alterations. The presence of infection was verified by microscopy and was confirmed by amplification of region small subunit (SSU) of ribosomal rNA using universal primers for microsporidia of Nosema sp. The purified PCR products were submitted to sequencing, and the sequences that had been obtained were edited and aligned with the sequences in a genbank database. In this way, it was possible to verify the presence of intracellular parasites in T. diatraeae, P. elaeisis and D. saccharalis pertaining to Clade Nosema/Vairimorpha. However, this is the first one report about detection of the microsporidia in the parasitoids T. diatraeae and P. elaeisis.

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Section: Resultsmentioning

confidence: 99%

Infection by the microsporidium of Clado Nosema/Vairimorpha in pupal parasitoids

Paes

Carvalho

Souza

et al. 2019

An. Acad. Bras. Ciênc.

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“…7 A). Lacewings are known for their symbiotic association with yeasts, bacteria and microsporidium [ 18 ]. Microsporidian spores have also been observed in the digestive organs of C. carnea .…”

Section: Discussionmentioning

confidence: 99%

“…In the green lacewing Chrysoperla carnea , all stages of microsporidium develop in direct contact with the host cell cytoplasm. Microsporidian spores were observed in cells of the proventriculus, diverticulum and in epithelial cells of the posterior midgut [ 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

Morphology of the male reproductive system and sperm ultrastructure of the green lacewing, Chrysopa pallens (Rambur, 1838) (Neuroptera: Chrysopidae)

Liu,

Guo,

Feng

et al. 2023

BMC Zool

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Background Chrysopa pallens is one of the most beneficial and effective natural predators, and is famous for its extensive distribution, wide prey spectrum, and excellent reproductive performance. This study examined the anatomy and fine structure of the C. pallens reproductive system and spermatogenesis. Results The male reproductive system of C. pallens comprises a pair of testes, a vas deferens, seminal vesicles, accessory glands, and short ejaculatory ducts. The testes were already mature on the day of emergence, but the accessory glands did not mature until 5 days post-emergence. In early spermatids, the flagellum had an axoneme on one side of the two mitochondrial derivatives. The nucleus was surrounded by parallel crystalline and paracrystalline materials. The spermatid envelope extends towards the paracrystalline material in a tail-shaped wing. In mature spermatids, the axoneme is located between the two accessory bodies and mitochondrial derivative sets. The parallel-crystalline and paracrystalline materials disappeared. In the testes, the wall of seminal cysts consists of a layer of epithelium, a muscular-connective sheath, and several vesicles of different sizes. The mature seminal cysts contained 128 spermatozoa. The accessory gland is composed of six parts: ventral papilla-like protuberance, anterior glandular lobe, lateral glandular lobe, seminal cyst, posterior kidney-shaped lobe, and posterior papilla-like protuberance. Muscle fibers and secretory granules are extensive. Conclusions This study provides information on the reproductive system of C. pallens and offers a resource for taxonomy and reproductive biology.

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Prevention and Management of Diseases in Terrestrial Invertebrates

Eilenberg

Jensen

2017

Ecology of Invertebrate Diseases

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Ultrastructure and molecular characterization of the microsporidium, Nosema chrysoperlae sp. nov., from the green lacewing, Chrysoperla carnea (Stephens) (Neuroptera: Chrysopidae) used for biological pest control

Cited by 6 publications

References 25 publications

Infection by the microsporidium of Clado Nosema/Vairimorpha in pupal parasitoids

Infection by the microsporidium of Clado Nosema/Vairimorpha in pupal parasitoids

Morphology of the male reproductive system and sperm ultrastructure of the green lacewing, Chrysopa pallens (Rambur, 1838) (Neuroptera: Chrysopidae)

Prevention and Management of Diseases in Terrestrial Invertebrates

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