High-fat feeding rapidly induces obesity and lipid derangements in C57BL/6N mice

Abstract

C57BL/6N (B6N) is becoming the standard background for genetic manipulation of the mouse genome. The B6N, whose genome is very closely related to the reference C57BL/6J genome, is versatile in a wide range of phenotyping and experimental settings and large repositories of B6N ES cells have been developed. Here, we present a series of studies showing the baseline characteristics of B6N fed a high-fat diet (HFD) for up to 12 weeks. We show that HFD-fed B6N mice show increased weight gain, fat mass, and hypercholesterolemia compared to control diet-fed mice. In addition, HFD-fed B6N mice display a rapid onset of lipid accumulation in the liver with both macro- and microvacuolation, which became more severe with increasing duration of HFD. Our results suggest that the B6N mouse strain is a versatile background for studying diet-induced metabolic syndrome and may also represent a model for early nonalcoholic fatty liver disease.Electronic supplementary materialThe online version of this article (doi:10.1007/s00335-013-9456-0) contains supplementary material, which is available to authorized users.

Taurine Treatment Modulates Circadian Rhythms in Mice Fed A High Fat Diet

“…However, the impact of taurine on circadian clocks has not been studied before. In our results, HFD treatment significantly increased body weight and visceral fat and decreased glucose tolerance and insulin sensitivity, as previously established in other studies102021. Taurine treatment prevents increases in body weight, glucose intolerance, insulin resistance and reduced visceral fat, according to studies where taurine had several beneficial metabolic effects in different models of type 2 diabetes and obesity2223242526.…”

Orange beverage ameliorates high-fat-diet-induced metabolic disorder in mice

“…They developed obesity, dyslipidaemia and glucose intolerance; therefore, they can be considered a convenient rodent model for MetS. These results were consistent with those previously reported by Fraulop, Ogg-Diamantino, Fernandes-Santos, Barbosa, and Mandarim-de-Lacerda (2010) and Podrini et al (2013) who used mouse models after consumption of a HFD.…”

d-Xylose suppresses adipogenesis and regulates lipid metabolism genes in high-fat diet–induced obese mice

“…In contrast, final body weight, body weight gain, body weight gain per day, FER (percentages), and subcutaneous and visceral fat mass were lowered for the Xylo 5 and Xylo 10 groups compared with the HFD group. Previous studies have suggested that HFD-induced obesity alters lipid and blood glucose metabolism based on their analysis of metabolic parameters such as TC, LDL-C, and HDL-C [37,38]. Increased levels of TC and LDL-C, accompanied by low levels of HDL-C, are major risk factors for atherosclerosis and cardiovascular disease [39].…”