Nosema ceranae induced mortality in honey bees (Apis mellifera) depends on infection methods

Milbrath, Meghan O.; Xie, Xianbing; Zhi, Huang

doi:10.1016/j.jip.2013.05.006

Cited by 11 publications

(5 citation statements)

References 14 publications

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“…Since the first report of N. ceranae infection in A. mellifera, there has been some controversy about the consequences of such infection. However, in recent years most studies have confirmed that N. ceranae has a pathogenic effect in this host, expressed at least in a shortening of the workers' lifespan in controlled (cage) experiment (e.g., Mayack and Naug, 2009;Alaux et al, 2010;Martín-Hern andez et al, 2011;Dussaubat et al, 2012;Goblirsch et al, 2013;Schwarz and Evans, 2013;Aufauvre et al, 2014;Basualdo et al, 2014;Roberts and Hughes, 2014;2015;Williams et al, 2014;Doublet et al, 2015aHuang et al, 2015; only few papers failing to report this effect (Milbrath et al, 2013;Retschnig et al, 2014;Garrido et al, 2016).…”

Section: Genetic Diversity Of N Ceranaementioning

confidence: 99%

Nosema ceranae in Apis mellifera: a 12 years postdetection perspective

Martín-Hernández

Bartolomé

Chejanovsky

et al. 2018

Environmental Microbiology

161

118

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Nosema ceranae is a hot topic in honey bee health as reflected by numerous papers published every year. This review presents an update of the knowledge generated in the last 12 years in the field of N. ceranae research, addressing the routes of transmission, population structure and genetic diversity. This includes description of how the infection modifies the honey bee's metabolism, the immune response and other vital functions. The effects on individual honey bees will have a direct impact on the colony by leading to losses in the adult's population. The absence of clear clinical signs could keep the infection unnoticed by the beekeeper for long periods. The influence of the environmental conditions, beekeeping practices, bee genetics and the interaction with pesticides and other pathogens will have a direct influence on the prognosis of the disease. This review is approached from the point of view of the Mediterranean countries where the professional beekeeping has a high representation and where this pathogen is reported as an important threat.

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Section: Genetic Diversity Of N Ceranaementioning

confidence: 99%

Nosema ceranae in Apis mellifera: a 12 years postdetection perspective

Martín-Hernández

Bartolomé

Chejanovsky

et al. 2018

Environmental Microbiology

161

118

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“…The methods used to feed bees Nosema spores can have variable effects on the final infection levels and on bee survival (Milbrath et al 2013;Urbieta-Magro et al 2019). Although we infected newly emerged bees and foragers (Table 4) and used both group-level spore feeding ( §2.4, §2.5) and individual bee feeding ( §2.6), the final spore loads were comparable between our experiments and were similar to those found in other studies (Paxton et al 2007;Maistrello et al 2008;Rubanov et al 2019).…”

Section: Discussionmentioning

confidence: 99%

Nicotine does not reduce Nosema ceranae infection in honey bees

et al. 2020

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Your article is protected by copyright and all rights are held exclusively by International Union for the Study of Social Insects (IUSSI). This e-offprint is for personal use only and shall not be self-archived in electronic repositories. If you wish to self-archive your article, please use the accepted manuscript version for posting on your own website. You may further deposit the accepted manuscript version in any repository, provided it is only made publicly available 12 months after official publication or later and provided acknowledgement is given to the original source of publication and a link is inserted to the published article on Springer's website. The link must be accompanied by the following text: "The final publication is available at link.springer.com".

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“…In previous studies, inoculum application was carried out after anesthesia with CO 2 to ensure that individual bees received all Nosema spores [29,[56][57]. However, this method was abandoned after it was reported that CO 2 anesthesia affected the mortality rates and lifespans of bees and caused stress due to keeping bees [58,59]. In recent years, individual inoculation in bees has been replaced by mass inoculation [60][61][62].…”

Section: Plos Onementioning

confidence: 99%

Production, characterization and therapeutic efficacy of egg yolk antibodies specific to Nosema ceranae

Açık,

Karagülle,

Yakut

et al. 2024

PLoS ONE

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Nosema disease, caused by Nosema ceranae, one of the single-celled fungal microsporidian parasites, is one of the most important and common diseases of adult honey bees. Since fumagillin, which has been used for decades in the control of Nosema disease in honey bees (Apis mellifera), poses a toxic threat and its efficacy against N. ceranae is uncertain, there is an urgent need to develop alternative prophylactic and curative strategies for the treatment of this disease. The main aim of this study was to investigate the therapeutic potential of specific egg yolk immunoglobulins (IgY) on Nosema disease. For this purpose, the presence of N. ceranae was determined by microscopic and PCR methods in honey bees collected from Nosema suspicious colonies by conducting a field survey. Layered Ataks chickens, divided into four groups each containing 20 animals, were vaccinated with live and inactivated vaccines prepared from field isolates of N. ceranae. Eggs were collected weekly for 10 weeks following the last vaccination. IgY extraction was performed using the PEG precipitation method from egg yolks collected from each group, and the purity of the antibodies was determined by SDS-PAGE and Western Blot. The presence of N. ceranae-specific IgYs was investigated by Western Blot and indirect ELISA methods. It was determined that specific IgYs showed high therapeutic efficacy on Nosema disease in naturally infected bee colonies. In addition, honey bees collected from infected colonies were brought to the laboratory and placed in cages with 30 bees each, and the effectiveness of IgYs was investigated under controlled conditions. It was detected that specific IgY reduced the Nosema spore load and the number of infected bees significantly in both the field and experimental study groups treated for seven days. It was concluded that chicken IgYs, an innovative and eco-friendly method, had a significant potential for use as an alternative to antifungal drugs.

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Nosema ceranae induced mortality in honey bees (Apis mellifera) depends on infection methods

Cited by 11 publications

References 14 publications

Nosema ceranae in Apis mellifera: a 12 years postdetection perspective

Nosema ceranae in Apis mellifera: a 12 years postdetection perspective

Nicotine does not reduce Nosema ceranae infection in honey bees

Production, characterization and therapeutic efficacy of egg yolk antibodies specific to Nosema ceranae

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