In vitro toxicity of silver nanoparticles to kiwifruit pollen exhibits peculiar traits beyond the cause of silver ion release

Speranza, Anna Maria; Crinelli, Rita; Scoccianti, Valeria; Taddei, Anna Rita; Iacobucci, Marta; Bhattacharya, Priyanka; Ke, Pu Chun

doi:10.1016/j.envpol.2013.04.021

Cited by 57 publications

(31 citation statements)

References 62 publications

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“…These particles are from about 5-50 nm, and their overall levels have been increased dramatically per unit volume of solution with various applications (Handy et al 2008;Rafiuddin 2012). Through the release of silver ions (Speranza et al 2013), AgNPs may play roles at various levels of plant metabolism. Thus, some studies have shown that the application of AgNPs can alter water parameters of the plant such as the transpiration rate and hydraulic conductivity, and these effects might result from the inhibitory action of silver ions on the production of ethylene.…”

Section: Introductionmentioning

confidence: 99%

The effect of colloidal silver nanoparticles on the level of lignification and hyperhydricity syndrome in Thymus daenensis vitro shoots: a possible involvement of bonded polyamines

Bernard

Moghadam

Mirzajani

2015

In Vitro Cell.Dev.Biol.-Plant

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Hyperhydricity syndrome (HHS) occurs when plants are stressed resulting in sharp changes in the ethylene concentration in the in vitro culture. One of the characteristics of HHS is the reduction of cell wall lignification. The aim of this study was to examine the effects of silver nanoparticles (AgNPs) on HHS in micropropagated Thymus daenensis shoots. AgNPs (0, 1, 2.5, or 5 mg L −1 ) were mixed with Murashige and Skoog (MS) culture medium and the antioxidant response and variations in lignin content were measured on micropropagated shoots derived from seedlings cultured with AgNPs. In addition, the different forms of microshoot polyamines (PAs) were investigated through High Performance Thin Layer Chromatography (HPTLC). The results indicated that seedling pretreatment with AgNPs at concentrations of 1 and 2.5 mg L −1 led to a decrease in the activity of the microshoot antioxidant system resulting in an increase of H 2 O 2 . Furthermore, the increased lignin content in response to the 2.5 mg L −1 AgNPs treatment was accompanied by an increase in the concentration of the bonded form of putrescine (Put) and spermine (Spm). There was a direct correlation between the lignin content and the concentration of bonded PAs. We hypothesize that the higher level of bonded PAs reduced the concentration of the free forms, leading to increased lignification. This may have led to the decreased HHS observed in shoot cultures.

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Section: Introductionmentioning

confidence: 99%

The effect of colloidal silver nanoparticles on the level of lignification and hyperhydricity syndrome in Thymus daenensis vitro shoots: a possible involvement of bonded polyamines

Bernard

Moghadam

Mirzajani

2015

In Vitro Cell.Dev.Biol.-Plant

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“…Current available techniques to assess in vitro toxicity of nanomaterials such as silica (Clément et al, 2013), silver nanoparticles (Speranza et al, 2013), carbon- (Gui et al, 2011) or metal-oxide-based (Vittori Antisari et al, 2013) rely on the functionality, affinity and/or selectivity of a biological recognition elements (e.g., biosensor, antibodies, cellular membrane, organelles or DNA etc.) as well as the processing power and detection capabilities of micro and optoelectronics (Mulchandani and Bassi, 1995; Zhao et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Electronic platform for real-time multi-parametric analysis of cellular behavior post-exposure to single-walled carbon nanotubes

Eldawud

Wagner

Dong

et al. 2015

Biosensors and Bioelectronics

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Single-walled carbon nanotubes (SWCNTs) implementation in a variety of biomedical applications from bioimaging, to controlled drug delivery and cellular-directed alignment for muscle myofiber fabrication, has raised awareness of their potential toxicity. Nanotubes structural aspects which resemble asbestos, as well as their ability to induce cyto and genotoxicity upon interaction with biological systems by generating reactive oxygen species or inducing membrane damage, just to name a few, have led to focused efforts aimed to assess associated risks prior their user implementation. In this study, we employed a non-invasive and real-time electric cell impedance sensing (ECIS) platform to monitor behavior of lung epithelial cells upon exposure to a library of SWCNTs with user-defined physicochemical properties. Using the natural sensitivity of the cells, we evaluated SWCNT-induced cellular changes in relation to cell attachment, cell–cell interactions and cell viability respectively. Our methods have the potential to lead to the development of standardized assays for risk assessment of other nanomaterials as well as risk differentiation based on the nanomaterials surface chemistry, purity and agglomeration state.

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“…suggesting a different type of interaction between pollen grains and tubes. Inhibition of pollen germination induced by Ag-nanoparticles has already been documented by Speranza et al, 25 who hypothesized a possible physical blockage of the transmembrane ion channels at the site of tube protrusion by the nanoparticles, preventing the formation of the inward ion current preceding pollen germination. 44 Alternatively, GO internalization at the site of the tube protrusion could have impaired the metabolic pathways triggering pollen germination.…”

Section: Effect Of Ph On Pollen Performancesmentioning

confidence: 93%

“…20 It was also used as an indicator of air pollution because the pollen performance, when described in terms of pollen germination and pollen tube elongation, can be affected (depending on species) by gaseous airborne pollutants such as NO x , O 3 and SO 2 . [21][22][23] More recently, it was demonstrated that also airborne particulate matter affects pollen performance: Speranza et al 24,25 found that Ag-and Pd-nanoparticles impair the in vitro pollen germination and the elongation of the pollen tube in the kiwifruit vine, Actinidia deliciosa C. F. Liang & A. R. Ferguson. Interestingly, this effect is derived from the nanoparticles themselves rather than from the ions carried on their surface.…”

Section: Introductionmentioning

confidence: 99%

“…Interestingly, this effect is derived from the nanoparticles themselves rather than from the ions carried on their surface. 24,25 Given these premises, our hypothesis is that the interaction of aerodispersed GBM flakes with pollen might impair the pollen performances affecting negatively the reproductive process of seed plants. To verify this, the effect of GO, the most reactive GBM tested so far, was verified on the pollen performance of two species, the entomophilous tobacco plant (Nicotiana tabacum L.) and the anemophilous common hazel (Corylus avellana L.), through in vitro germination experiments.…”

Section: Introductionmentioning

confidence: 99%

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Graphene oxide impairs the pollen performance of Nicotiana tabacum and Corylus avellana suggesting potential negative effects on the sexual reproduction of seed plants

Carniel

Gorelli

Flahaut

et al. 2018

Environ. Sci.: Nano

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The production of graphene based materials (GBMs) is steadily increasing but the effects of the possible release of GBMs in the environment are far from being understood. Graphene oxide (GO) is among the most active GBMs and it causes widely varying effects on the vegetative body of seed plants. However, nothing is known yet about its potential effects on the reproductive process. This study addresses the effects of GO on pollen germination and pollen tube elongation in the model species Nicotiana tabacum and in the non-model species Corylus avellana. In vitro germination experiments were conducted without or with GO (control and treated samples, respectively) at concentrations of 25, 50 and 100 μg mL . Pollen germination and tube elongation were affected at GO concentrations ≥50 μg mL ) samples demonstrated that the main factor influencing the pollen performance is the acidic properties of GO. This might affect the reproductive process of numerous seed plants thus being relevant from an environmental point of view.

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In vitro toxicity of silver nanoparticles to kiwifruit pollen exhibits peculiar traits beyond the cause of silver ion release

Cited by 57 publications

References 62 publications

The effect of colloidal silver nanoparticles on the level of lignification and hyperhydricity syndrome in Thymus daenensis vitro shoots: a possible involvement of bonded polyamines

The effect of colloidal silver nanoparticles on the level of lignification and hyperhydricity syndrome in Thymus daenensis vitro shoots: a possible involvement of bonded polyamines

Electronic platform for real-time multi-parametric analysis of cellular behavior post-exposure to single-walled carbon nanotubes

Graphene oxide impairs the pollen performance of Nicotiana tabacum and Corylus avellana suggesting potential negative effects on the sexual reproduction of seed plants

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