Cell type-selective expression of the zinc finger-containing gene Nolz-1/Zfp503 in the developing mouse striatum

Ectopic Expression of Nolz-1 in Neural Progenitors Promotes Cell Cycle Exit/Premature Neuronal Differentiation Accompanying with Abnormal Apoptosis in the Developing Mouse Telencephalon

“…Notably, unlike the restricted expression of Nolz-1 to post-mitotic neurons in developing striatum in?vivo [11], [14], it has been reported that Nolz-1 is expressed in proliferative neural progenitors in neurosphere culture in which over-expression of hNolz-1 results in increases of cell cycle exit [10]. On the other hand, functional studies of elbow ( elB ) and no ocelli ( noc ), two Drosophila homologous genes of Nolz-1 , indicate regulatory control of cell proliferation by elB and noc [33], [34].…”

“…The membranes were cut into 2–3 pieces for detection of different proteins according to the molecular weights of the proteins, Nolz-1 (∼65 kDa), TuJ1 (∼50 kDa), PCNA (∼29 kDa), cleaved caspase 3 (∼17/19 kDa), α-tubulin (∼50 kDa) and β-actin (∼42 kDa) proteins. The primary antibodies were rabbit anti-Nolz-1 (1∶1,000) [14], mouse anti-TuJ1 (1∶10,000, Promega, Madison WI), rabbit anti-cleaved caspase 3 (1∶1,000, Cell Signaling Technology, Danvers, MA), mouse anti-PCNA (1∶1,000, Santa Cruz Biotechnology, Dallas, Texas), mouse anti-α-tubulin (1∶1,000, Sigma Aldrich, St. Louis, Missouri) and mouse anti-β-actin (1∶10,000; Sigma Aldrich). Secondary antibody used was horseradish peroxidase conjugated goat anti-rabbit IgG and goat anti-mouse IgG (1∶10,000; Jackson ImmunoResearch Laboratories, West Grove, PA).…”

“…Nolz-1 expression level is lower in the differentiated MZ. Moreover, Nolz-1 is not expressed in Ki67-positive proliferating progenitor cells but is co-expressed with the early neuronal differentiation markers of TuJ1 and Isl-1 indicating that Nolz-1 is expressed in early post-mitotic striatal neurons [10], [11], [14]. Nolz-1 therefore serves as a developmental marker for differentiating striatal neurons.…”

Selective neuronal expression of the SoxE factor, Sox8, in direct pathway striatal projection neurons of the developing mouse brain

“…A number of developmental control factors including Gsx1/2, Ascl1, and Dlx genes are expressed by LGE progenitors and have been shown to be required for the normal development of LGEderived neuronal subtypes, including the SPNs (Anderson et al, 1997;Casarosa, Fode, & Guillemot, 1999;Corbin, Gaiano, Machold, Langston, & Fishell, 2000;Long et al, 2009;Pei et al, 2011;Toresson & Campbell, 2001;Toresson, Potter, & Campbell, 2000;Waclaw, Wang, Pei, Ehrman, & Campbell, 2009;Wang, Lufkin, & Rubenstein, 2011;Wang, Waclaw, Allen, Guillemot, & Campbell, 2009;Wang et al, 2013;Yun, Garel, Fischman, & Rubenstein, 2003;Yun, Potter, & Rubenstein, 2001;Yun et al, 2002). Transcription factors that mark differentiating SPNs in both the indirect and direct pathways include Nolz1/Zfp503, Ctip2, and Foxp1 (Arlotta, Molyneaux, Jabaudon, Yoshida, & Macklis, 2008;Chang et al, 2004;Ko, Chen, Chen, Hao, & Liu, 2013;Tamura, Morikawa, Iwanishi, Hisaoka, & Senba, 2004). Moreover, Ctip2 and Foxp1 have been shown to be required for the normal differentiation of SPNs (Arlotta et al, 2008;Bacon et al, 2015).…”

The Transcriptional Repressor ZNF503/Zeppo2 Promotes Mammary Epithelial Cell Proliferation and Enhances Cell Invasion

“…In zebrafish, the ZPO2 homologous protein Nlz2 (Znf503) is required for proper hindbrain development (2). In mice, expression of Zpo2 is detected during limb and striatum development, highlighting the importance of Zpo2 during organogenesis (3,4). Although these examples demonstrate that Zpo2 plays key roles in multiple developmental processes, the role of Zpo2 in adult tissue (homeostasis) remains elusive.…”