Dried apple enriched with mandarin juice counteracts tamoxifen-induced oxidative stress in rats

Abstract

Codoñer Franch, P.; Betoret Valls, ME.; López Jaén, AB.; Betoret Valls, N.; Fito Maupoey, P.; Valls Bellés, MV. (2013). Dried apple enriched with mandarin juice counteracts tamoxifen induced oxidative stress in rats. International Journal of Food Sciences and Nutrition. 64(7): 815-821. doi:10.3109/09637486.2013.798267. Dried apple enriched with mandarin juice by vacuum impregnation counteracts the liver oxidative effect of tamoxifen in rats. AbstractWe have investigated the effects of a product made of apple dehydrated enriched by vacuum impregnation of mandarin juice in decreasing oxidative stress biomarkers in rat induced liver injury by tamoxifen. A experimental design was undertaken in six groups of animals. One group fed a standard diet. Two groups were supplemented with 0.745 g/d of apples snacks/day either impregnated or not with mandarin juice during 28 days. Three groups of rats were treated with tamoxifen 1.54 mg/kg/day during 21 days; of these, one group was supplemented with the apple snacks and the other with the apple snacks impregnated with mandarin juice. Plasma aminotransferases were determined as index of hepatocellular damage and interleukin-6 as measure of inflammation. Plasma and liver carbonyl groups, indicative of protein oxidation, and α-tocopherol as evaluation of antioxidant defense were measured. The modified base 8-hydroxydexyguanosine as indicative of DNA damage was determined in liver. Tamoxifen induced an increase in aminotranferases and both plasma and liver markers of oxidative stress and DNA damage that decrease significantly after apple snack consumption; in most of cases they even reach normal values as those achieved previous to induce the injury with tamoxifen. The effect of the mandarin juice incorporated to the structure of apple mainly accounts for increasing levels of α-tocopherol in plasma and liver. The present study shown the potential protective action of the products made of dehydrated apple either impregnated or not with mandarin juice by vacuum impregnation for counteracting the chemically induced liver injury in rats.

Onion and Apple Functional Ingredients Intake Improves Antioxidant and Inflammatory Status and Vascular Injury in Obese Zucker Rats

“…The ABTS assay measures the relative ability of antioxidants to scavenge the ABTS •+ generated in aqueous phase and is reactive towards most antioxidants including thiols. In line with the present results, Codoñer-Franch et al (2013) [ 52 ] showed that supplementation with an apple snack in tamoxifen-treated rats significantly increased the plasma antiradical activity measured by ABTS •+ and FRAP methods. On the contrary, Aprikian et al (2002) [ 15 ] reported that the apple diet did not affect the plasma FRAP value in lean or obese rats.…”

Interactions Between Natural Products and Tamoxifen in Breast Cancer: A Comprehensive Literature Review

“…Rats treated with TAM showed elevated aminotransaminase, carbonyl groups (CGs), and 8-hydroxydeoxyguanosine (8OHdG) ( Codoñer-Franch et al, 2013 ). When dried apple snack enriched with mandarin juice was used in combination with TAM, restoration of oxidative damage to protein and DNA in liver were observed with a significant reduction in plasma and liver markers of oxidative stress recorded, exhibiting a protective effect on the oxidative stress induced by TAM in rats ( Codoñer-Franch et al, 2013 ).…”

Cautiously using natural medicine to treat liver problems

“…Apple and mandarin are also rich in antioxidants. An animal study confirmed that a diet made of dried apple and mandarin orange is protective against liver impairment induced by tamoxifen in rats[34]. Another study revealed the hepatoprotective efficacy of apple pomace aqueous extract, as it had inhibited CCl 4 -induced hepatocyte apoptosis[35].…”