Exhaustion of Racing Sperm in Nature‐Mimicking Microfluidic Channels During Sorting

Tasoğlu, Savaş; Safaee, Hooman; Zhang, Xiaohui; Kingsley, James L.; Catalano, Paolo N.; Gürkan, Umut A.; Nureddin, Aida; Kayaalp, Emre; Anchan, Raymond M.; Maas, Richard L.; Tüzel, Erkan; Demirci, Utkan

doi:10.1002/smll.201300020

Cited by 106 publications

(93 citation statements)

References 46 publications

(47 reference statements)

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“…Figure 2 a,b illustrates trajectories for the 30 × 26 µm channels. At the inlet and outlet, sperm trajectories and kinematic parameters, including straight‐line velocity (VSL) and curvilinear velocity (VCL), were measured, and compared with blank microfluidic channels (BMC) 3. As shown in Figure 2c, VSL values at the outlets of SPARTAN were higher than those at the inlet (Table S1, Supporting Information), consistent with the predictions from the multiscale model (Figure 2d).…”

Section: Resultssupporting

confidence: 73%

“…Further analysis using the PRW model reveals a large enhancement of the distance traversed by the sperm in SPARTAN, compared to the BMC 3. More specifically, the persistence length, L p , for normal sperm is 5‐ and 20‐fold larger than that of sperm with large head and bent neck, respectively (Figure 3d).…”

Section: Resultsmentioning

confidence: 95%

“…Many such cases are treated with assisted reproduction technologies (ARTs),2, 3 most commonly using in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) 4. During ART procedures, semen is traditionally processed with the density gradient centrifugation or swim‐up techniques 5, 6.…”

Section: Introductionmentioning

confidence: 99%

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Guidance and Self‐Sorting of Active Swimmers: 3D Periodic Arrays Increase Persistence Length of Human Sperm Selecting for the Fittest

et al. 2017

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Male infertility is a reproductive disease, and existing clinical solutions for this condition often involve long and cumbersome sperm sorting methods, including preprocessing and centrifugation‐based steps. These methods also fall short when sorting for sperm free of reactive oxygen species, DNA damage, and epigenetic aberrations. Although several microfluidic platforms exist, they suffer from structural complexities, i.e., pumps or chemoattractants, setting insurmountable barriers to clinical adoption. Inspired by the natural filter‐like capabilities of the female reproductive tract for sperm selection, a model‐driven design, featuring pillar arrays that efficiently and noninvasively isolate highly motile and morphologically normal sperm, with lower epigenetic global methylation, from raw semen, is presented. The Simple Periodic ARray for Trapping And isolatioN (SPARTAN) created here modulates the directional persistence of sperm, increasing the spatial separation between progressive and nonprogressive motile sperm populations within an unprecedentedly short 10 min assay time. With over 99% motility of sorted sperm, a 5‐fold improvement in morphology, 3‐fold increase in nuclear maturity, and 2–4‐fold enhancement in DNA integrity, SPARTAN offers to standardize sperm selection while eliminating operator‐to‐operator variations, centrifugation, and flow. SPARTAN can also be applied in other areas, including conservation ecology, breeding of farm animals, and design of flagellar microrobots for diagnostics.

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Section: Resultssupporting

confidence: 73%

Section: Resultsmentioning

confidence: 95%

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Guidance and Self‐Sorting of Active Swimmers: 3D Periodic Arrays Increase Persistence Length of Human Sperm Selecting for the Fittest

et al. 2017

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“…1) have led to the design of ratchet devices that guide and sort self-propelled cells using asymmetric obstacles [16,17]. In particular, different microfluidic devices have been created to either increase sperm cell quality or enhance their concentration [18][19][20]. The creation of inhomogeneous distributions of swimmer populations via asymmetric obstacles has been shown to be particularly efficient for run-and-tumble bacteria [16,[21][22][23].…”

mentioning

confidence: 99%

Geometrical guidance and trapping transition of human sperm cells

et al. 2014

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The guidance of human sperm cells under confinement in quasi 2D microchambers is investigated using a purely physical method to control their distribution. Transport property measurements and simulations are performed with dilute sperm populations, for which effects of geometrical guidance and concentration are studied in detail. In particular, a trapping transition at convex angular wall features is identified and analyzed. We also show that highly efficient microratchets can be fabricated by using curved asymmetric obstacles to take advantage of the spermatozoa specific swimming strategy.PACS numbers: 87.17. Jj, 87.18.Hf, 87.17.Aa, 87.17.Rt Understanding sperm dynamics under confining microgeometries is a general problem and a major challenge both from the basic biophysics and the complex fluids points of view. It is also crucial for microfluidics and biomedical control applications. Our knowledge of the swimming cell motilities in unbounded media cannot be directly extrapolated to their behavior in complex environments such as those found in the oviduct or in the labon-a-chip microfluidic devices used to control and analyze small samples or for in-vitro reproduction procedures. In these cases, the characteristic length scales are of the same order as the cell size, i.e. a few micrometers. Under these circumstances, confined self-propelled microorganisms undergo substantial changes in their locomotion habits, adapting their dynamics to intricate porous media or to solid surfaces vicinity [1], reducing their speed close to boundaries [2] or adjusting their morphology and motility in very narrow channels [3].It has been shown that microswimmers with very different propulsion systems are similarly attracted to the walls and to swim parallel to the surface [2,[4][5][6][7][8][9][10][11]. It is believed that this attractive force has hydrodynamic origin although other possible mechanisms have been proposed [12][13][14]. Several models have been introduced to describe the swimming along surfaces (see Ref.[15] and references therein). Interestingly, the direct observation of the cell-wall attraction (see Fig. 1) have led to the design of ratchet devices that guide and sort self-propelled cells using asymmetric obstacles [16,17]. In particular, different microfluidic devices have been created to either increase sperm cell quality or enhance their concentration [18][19][20]. The creation of inhomogeneous distributions of swimmer populations via asymmetric obstacles has been shown to be particularly efficient for run-and-tumble bacteria [16,[21][22][23]. Alternative ways of achieving nonuniform distributions have also been obtained combining symmetric funnels and flux [24]. Nowadays, numerous theoretical treatments are available to account for the effects of asymmetric obstacles on active particles distributions [25][26][27][28]. Tumbles, rotational diffusion and collisions are efficient mechanisms for separating the cells from the surface, thus permitting bacteria to be reinserted into the bulk of the confining micro...

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“…These microfluidic devices can be used by pipetting sample liquid without complex fluid manipulation systems, such as electric pumps and bulbs, leading to the advantages of simplicity and low cost. Since the 1990s, microfluidic devices for sperm counts and motility analyses have been investigated by Kricka et al [39], and microfluidic POC systems for semen diagnostics sorting motile sperms or on-chip IVF using LOC have been reported [40][41][42][43][44][45][46][47][48][49][50][51]. Moreover, in addition to motile sperm separation, fluid flow-and viscosity-associated chemotaxis and mechanotaxis of mammalian sperm were investigated using microfluidic channels [45].…”

Section: Microfluidic Channel-based Devices For Semen Diagnoses and Mmentioning

confidence: 99%

Recent advances in microfluidic diagnostic and treatment systems for assisted reproductive technologies in developing countries

Matsuura¹,

Nishina²,

Asano³

2017

Point-of-Care Diagnostics - New Progresses and Perspectives

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Exhaustion of Racing Sperm in Nature‐Mimicking Microfluidic Channels During Sorting

Cited by 106 publications

References 46 publications

Guidance and Self‐Sorting of Active Swimmers: 3D Periodic Arrays Increase Persistence Length of Human Sperm Selecting for the Fittest

Guidance and Self‐Sorting of Active Swimmers: 3D Periodic Arrays Increase Persistence Length of Human Sperm Selecting for the Fittest

Geometrical guidance and trapping transition of human sperm cells

Recent advances in microfluidic diagnostic and treatment systems for assisted reproductive technologies in developing countries

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