Transport of NaYF4:Er3+, Yb3+up-converting nanoparticles into HeLa cells

Sikora, Bożena; Fronc, K.; Kamińska, Izabela; Koper, Kamil; Szewczyk, Sebastian; Paterczyk, Bohdan; Wojciechowski, Tomasz; Sobczak, Kamil; Minikayev, R.; Paszkowicz, W.; Stępień, Piotr P.; Elbaum, Danek

doi:10.1088/0957-4484/24/23/235702

Cited by 31 publications

(56 citation statements)

References 52 publications

(87 reference statements)

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“…The most accepted theory is that nanoparticles are internalized via endosome-mediated transport or through ribosome exchanges. 33 In general, the red emission from nanoparticles is strong in all images, which demonstrates the efficient luminescence generated by the nanoparticles synthesized in this work. It is worth noting that there was no autofluorescence from the cells after exciting the UPC nanoparticles with 970 nm.…”

Section: Imaging Of Zro 2 ∶Yb-er Nanocrystals Incubatedmentioning

confidence: 53%

“…These nanomaterials were internalized in HeLa cells observing visible light from the nanoparticles under IR excitation. [32][33][34][35][36] Though these platforms are efficient for labeling HeLa cells, they still show several problems related to the complexity of their fabrication. For example, the synthesis of NaGdF 4 ∶Yb 3þ , Er 3þ nanoparticles have some drawbacks for biomedical applications; therefore, gold or silica need to be used to render them with biocompatible properties.…”

Section: Introductionmentioning

confidence: 99%

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Labeling of HeLa cells using ZrO 2 : Yb 3 + - Er 3 + nanoparticles with upconversion emission

et al. 2015

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Abstract. This work reports the synthesis, structural characterization, and optical properties of ZrO 2 ∶Yb 3þ -Er 3þ (2-1 mol%) nanocrystals. The nanoparticles were coated with 3-aminopropyl triethoxysilane (APTES) and further modified with biomolecules, such as Biotin-Anti-rabbit (mouse IgG) and rabbit antibody-AntiKi-67, through a conjugation method. The conjugation was successfully confirmed by Fourier transform infrared, zeta potential, and dynamic light scattering. The internalization of the conjugated nanoparticles in human cervical cancer (HeLa) cells was followed by two-photon confocal microscopy. The ZrO 2 ∶Yb 3þ -Er 3þ nanocrystals exhibited strong red emission under 970-nm excitation. Moreover, the luminescence change due to the addition of APTES molecules and biomolecules on the nanocrystals was also studied. These results demonstrate that ZrO 2 ∶Yb 3þ -Er 3þ nanocrystals can be successfully functionalized with biomolecules to develop platforms for biolabeling and bioimaging. © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Section: Imaging Of Zro 2 ∶Yb-er Nanocrystals Incubatedmentioning

confidence: 53%

Section: Introductionmentioning

confidence: 99%

Labeling of HeLa cells using ZrO 2 : Yb 3 + - Er 3 + nanoparticles with upconversion emission

et al. 2015

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“…1). I n o r d e r t o o b t a i n t h e U C N P s w i t h g o o d biocompatibility, "one step" method was developed in recent years with the help of hydrophilic or binary cooperative ligands, such as polyols [10,35,36], EDTA [37][38][39][40], citrate [37,[41][42][43][44][45], sodium dodecyl sulfate (SDS) [46], PVP [47], small-molecule binary acid [48,49], polyethylene glycol (PEG) [49], PVP [50], PAA [51,52], PEI [51], 3-mercaptopropionic acid (3MA) and 6-aminocaproic acid (6AA) [53] etc. This "one step" method has the advantages of high efficiency and could be operated at mild reaction conditions [54,55].…”

Section: Synthesis Of Rare Earth Doped Ucnpsmentioning

confidence: 99%

Up-Conversion Nanoparticles for Gastric Cancer Targeted Imaging and Therapy

Yang¹,

Han²,

Yue³

2016

Nano BioMed ENG

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As a new generation of fluorophores, up-conversion nanoparticles (UCNPs) exhibit high penetration depth in tissues, low photodamage to biological samples, large Stokes shifts, sharp emission bands, free auto-fluorescence background and high photostability, thus making them an ideal kind of fluorescent labels for a variety of assay formats ranging from bio-imaging to cancer therapy. In this article, the method for the synthesis of biocompatible UCNPs, the recent progress regarding the UCNPs for multimodal targeted tumor imaging, drug delivery, photodynamic therapy (PDT), and photothermal therapy (PTT) are discussed on emphasis. The challenges and opportunities in this emerging field are also presented.

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“…Nanoparticles' ability to be absorbed by cells via endocytosis is one of the possible mechanisms of UCNPs influence on the cells. The literature data suggest the possibility of nanoparticles absorption by cell lines [16][17][18]. Active UCNPs internalization by primary hippocampal cells was not found, therefore, it could be assumed that cytotoxic effects of nanoparticles are caused by the UCNPs interaction with cell surface proteins.…”

Section: Neuroscience Researchmentioning

confidence: 99%

The Influence of Different Types of Upconversion Nanoparticles Surface Coatings on Neurotoxicity

et al. 2016

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The aim of the investigation was to study the effect of upconversion nanoparticles (UCNPs) with different surface coatings on viability and functional neural network activity of primary hippocampal cultures.Materials and Methods. The UCNPs with three surface coating modifications (tetramethylammonium hydroxide (TMAH), polymaleic acid octadecene and polyethyleneimine (PEI)) were synthesized by a solvothermal technique with further hydrophilization. Primary hippocampal cultures, obtained from C57BL/6 mice embryos (Е18), were incubated with tested UCNPs in the concentration of 0.8 and 8 mg/ml during 72 h. The cell viability detection, evaluation of morphological changes by immunocytochemical staining as well as the UCNPs influence on the main parameters of the functional neural network calcium activity and on the endocytosis processes were carried out.Results. Our studies revealed that UCNPs caused a dose-dependent cytotoxic effect on primary hippocampal cultures, wherein the severity of this effect directly related to the type of UCNPs surface coating. The greatest cytotoxicity was identified for UCNP-PEI, and the least -for UCNP-TMAH. UCNPs toxicity is manifested in significant morphological changes of neural networks and in the increase the number of dead cells (р<0.05) in primary hippocampal cultures. Moreover, a significant decrease (р<0.05) in the main parameters of spontaneous functional calcium activity was shown.Conclusion. A comprehensive investigation of the nanoparticles effects on primary hippocampal cultures showed that all tested UCNPs have the strong toxic effect to the nervous system cells.

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Transport of NaYF₄:Er³⁺, Yb³⁺up-converting nanoparticles into HeLa cells

Cited by 31 publications

References 52 publications

Labeling of HeLa cells using ZrO 2 : Yb 3 + - Er 3 + nanoparticles with upconversion emission

Labeling of HeLa cells using ZrO 2 : Yb 3 + - Er 3 + nanoparticles with upconversion emission

Up-Conversion Nanoparticles for Gastric Cancer Targeted Imaging and Therapy

The Influence of Different Types of Upconversion Nanoparticles Surface Coatings on Neurotoxicity

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