Growth‐associated protein‐43 expression in cocultures of dorsal root ganglion neurons and skeletal muscle cells with different neurotrophins

Zhang, Weiwei; Miao, Yajun; Xing, Ziying; Li, Hao; Liu, Huangxiang; Li, Zhenzhong

doi:10.1002/mus.23689

Cited by 5 publications

(1 citation statement)

References 31 publications

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“…Many experimental results showed that the secretion of neurotrophic factors is reduced after peripheral nerve injury, and an exogenous neurotrophic factor 3 (neurotrophin 3, NT-3) promotes nerve regeneration and protects against muscle atrophy [9,10]. However, the mechanism of such effects remains unclear.…”

Section: Introductionmentioning

confidence: 99%

Effects of Neurotrophin-3 Plasmids on Myocyte Apoptosis and Ca2+-ATPase Content in the Muscle After Nerve Injury in Rats

et al. 2015

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We estimated the influence of plasmids with DNA carrying the neurotrophin-3 (NT-3) gene on apoptosis in the gastrocnemius muscle and content of Ca 2+ -ATPase in the latter after sciatic nerve injury. Sixty adult Wistar rats were randomly divided into the saline (control) and NT-3 groups. The related indices, such as expression of caspase-3 protein, the rate of apoptosis in the muscle evaluated using a TUNEL technique, and the level of Ca 2+ -ATPase estimated using Western blot, were observed. Expression of caspase-3 protein was elevated at different post-operative times after peripheral nerve injury; NT-3 expression and the rate of muscle cell apoptosis decreased, whereas the level of Ca 2+ -ATPase in the sarcoplasmic reticulum increased; significant differences were observed compared with the saline group (P < 0.05). The mitigation mechanism of NT-3 on muscle atrophy after peripheral nerve injury is expressed as inhibition of caspase-3 gene expression, increase in the Ca 2+ -ATPase level, and reduction in the rate of muscle apoptosis.

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Section: Introductionmentioning

confidence: 99%

Effects of Neurotrophin-3 Plasmids on Myocyte Apoptosis and Ca2+-ATPase Content in the Muscle After Nerve Injury in Rats

et al. 2015

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Specific effects of neuregulin-1β on the communication between DRG neurons and skeletal muscle cells in vitro

Cong

Qiao

et al. 2018

J Muscle Res Cell Motil

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The communication between primary afferent neuron and skeletal muscle (SKM) is one of the important factors on maintaining the structure and function of SKM cells. Neuregulin-1β (NRG-1β) signaling is essential for regulating synaptic neurotransmission. Here, we established a neuromuscular coculture model of dorsal root ganglion (DRG) sensory neurons and SKM cells to explore the nerve-muscle communication in the presence of exogenous NRG-1β. The expression of three distinct subtypes (TrkA, TrkB, and TrkC) of tyrosine kinase receptors was monitored for the phenotypical alterations of the neurons. The aggregation extent of acetylcholine receptor (AChR) represents the specific changes of SKM cells after NRG-1β incubation in this neuromuscular coculture model. The results showed that NRG-1β not only enhanced neurite outgrowth of DRG neurons but also increased the length and branches of SKM cells. NRG-1β treatment not only induced expression of all the three subtypes of Trk receptors in neurons but also promoted AChR aggregation on the surface of SKM cells. The effects of NRG-1β could be blocked by administration of ERK1/2 inhibitor PD98059, PI3K inhibitor LY294002, and JAK2 inhibitor AG490, respectively. These data imply that NRG-1β is essential for the nerve-muscle communication by enhancing growth and modifying phenotypes of the two different kinds of cells. The specific effects produced by NRG-1β add novel interpretation for nerve-muscle communication between sensory neurons and SKM cells.

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The combination of db-cAMP and ChABC with poly(propylene carbonate) microfibers promote axonal regenerative sprouting and functional recovery after spinal cord hemisection injury

Xia

Huang

et al. 2017

Biomedicine & Pharmacotherapy

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Growth‐associated protein‐43 expression in cocultures of dorsal root ganglion neurons and skeletal muscle cells with different neurotrophins

Abstract: These results offer new clues for a better understanding of the effects of distinct NTs on GAP-43 expression in DRG sensory neurons in the presence of target SKM cells and implicate NTs and target SKM cells in DRG neuronal regeneration.

Cited by 5 publications

References 31 publications

Effects of Neurotrophin-3 Plasmids on Myocyte Apoptosis and Ca2+-ATPase Content in the Muscle After Nerve Injury in Rats

Effects of Neurotrophin-3 Plasmids on Myocyte Apoptosis and Ca2+-ATPase Content in the Muscle After Nerve Injury in Rats

Specific effects of neuregulin-1β on the communication between DRG neurons and skeletal muscle cells in vitro

The combination of db-cAMP and ChABC with poly(propylene carbonate) microfibers promote axonal regenerative sprouting and functional recovery after spinal cord hemisection injury

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