Evaluation of Brucella abortus S19 vaccines commercialized in Brazil: Immunogenicity, residual virulence and MLVA15 genotyping

Miranda, Karina Leite; Poester, Fernando Padilla; Minharro, Sílvia; Dorneles, Elaine Maria Seles; Stynen, Ana Paula Reinato; Lage, Andrey Pereira

doi:10.1016/j.vaccine.2013.04.054

Cited by 15 publications

(10 citation statements)

References 13 publications

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“…However, before being used for those purposes, PCR methods for molecular typing require careful in-house validation of typeability, reproducibility, repeatability, stability, discriminatory power and epidemiologic concordance [5,21]. The findings of this study, associated with previous data on the high discriminatory power and epidemiologic concordance of MLVA16, besides its good typeability and in vitro stability [6,7,9-11,13,15-17], corroborate the use of MLVA16 as suitable typing method for refining the understanding of the epidemiology of bovine brucellosis.…”

Section: Discussionsupporting

confidence: 63%

“…Likewise, the analysis of B. abortus S19 vaccine strain from different batches of different manufacturers did not reveal significant differences in MLVA16 pattern [17]. Concerning the evaluation of in vivo genetic stability of MLVA16 loci , it has also been demonstrated that passage of B. abortus RB51 in cattle and of B. abortus 2308 in mouse did not lead to changes in any marker of MLVA16 [9].…”

Section: Discussionmentioning

confidence: 99%

“…The genetic stability can be deduced by typing results of multiple isolates originating from the same strain or strains after numerous passages [8,9,15,16]. In in vitro evaluations, B. abortus vaccine and field strains have been shown to be stable by MLVA16 [9,15-17]. Regarding to in vivo stability of the MLVA16 markers, data based on B. abortus experimental infection showed only few changes in the hyper-variable loci [9].…”

Section: Introductionmentioning

confidence: 99%

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Genetic stability of Brucella abortus isolates from an outbreak by multiple-locus variable-number tandem repeat analysis (MLVA16)

et al. 2014

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BackgroundBrucellosis caused by Brucella abortus is one of the most important zoonoses in the world. Multiple-locus variable-number tandem repeat analysis (MLVA16) has been shown be a useful tool to epidemiological traceback studies in B. abortus infection. Thus, the present study aimed (i) to evaluate the genetic diversity of B. abortus isolates from a brucellosis outbreak, and (ii) to investigate the in vivo stability of the MLVA16 markers.ResultsThree-hundred and seventy-five clinical samples, including 275 vaginal swabs and 100 milk samples, were cultured from a brucellosis outbreak in a cattle herd, which adopted RB51 vaccination and test-and-slaughter policies. Thirty-seven B. abortus isolates were obtained, eight from milk and twenty-nine from post-partum/abortion vaginal swabs, which were submitted to biotyping and genotyping by MLVA16. Twelve B. abortus isolates obtained from vaginal swabs were identified as RB51. Twenty four isolates, seven obtained from milk samples and seventeen from vaginal swabs, were identified as B. abortus biovar 3, while one isolate from vaginal swabs was identified as B. abortus biovar 1. Three distinct genotypes were observed during the brucellosis outbreak: RB observed in all isolates identified as RB51; W observed in all B. abortus biovar 3 isolates; and Z observed in the single B. abortus biovar 1 isolate. Epidemiological and molecular data show that the B. abortus biovar 1 genotype Z strain is not related to the B. abortus biovar 3 genotype W isolates, and represents a new introduction B. abortus during the outbreak.ConclusionsThe results of the present study on typing of multiple clinical B. abortus isolates from the same outbreak over a sixteen month period indicate the in vivo stability of MLVA16 markers, a low genetic diversity among B. abortus isolates and the usefulness of MLVA16 for epidemiological studies of bovine brucellosis.

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Section: Discussionsupporting

confidence: 63%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genetic stability of Brucella abortus isolates from an outbreak by multiple-locus variable-number tandem repeat analysis (MLVA16)

et al. 2014

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“…achieved by multi- locus variable number of tandem repeats (VNTR) analyses (MLVA) have proved to be a powerful tool to determine relationships among Brucella spp isolates from different animal species and from humans, as well as for epidemiological trace-back studies [11-17]. However, data regarding B. ovis genotyping, using MLVA16 or even other techniques are very scarce.…”

Section: Resultsmentioning

confidence: 99%

Genetic diversity of Brucella ovis isolates from Rio Grande do Sul, Brazil, by MLVA16

Dorneles

Freire

Dasso³

et al. 2014

BMC Res Notes

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BackgroundOvine epididymitis is predominantly associated with Brucella ovis infection. Molecular characterization of Brucella spp. achieved by multi-locus variable number of tandem repeats (VNTR) analyses (MLVA) have proved to be a powerful tool for epidemiological trace-back studies. Thus, the aim of this study was to evaluate the genetic diversity of Brucella ovis isolates from Rio Grande do Sul State, Brazil, by MLVA16.FindingsMLVA16 genotyping identified thirteen distinct genotypes and a Hunter-Gaston diversity index of 0.989 among the fourteen B. ovis genotyped strains. All B. ovis MLVA16 genotypes observed in the present study represented non-previously described profiles. Analyses of the eight conserved loci included in panel 1 (MLVA8) showed three different genotypes, two new and one already described for B. ovis isolates. Among ten B. ovis isolates from same herd only two strains had identical pattern, whereas the four isolates with no epidemiologic information exhibited a single MLVA16 pattern each. Analysis of minimal spanning tree, constructed using the fourteen B. ovis strains typed in this study together with all nineteen B. ovis MLVA16 genotypes available in the MLVAbank 2014, revealed the existence of two clearly distinct major clonal complexes.ConclusionsIn conclusion, the results of the present study showed a high genetic diversity among B. ovis field isolates from Rio Grande do Sul State, Brazil, by MLVA16.

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“…vaccines is usually based on in vitro criteria, including physico-chemical and microbiological tests as purity, dissociation, determination of pH, humidity and count of viable bacteria. Nevertheless, genetic stability has recently been proposed as an additional criterion in assessing the quality of B. abortus vaccines [40,41]. Immunogenicity test performed in mouse is another alternative to assess the quality of bovine brucellosis vaccines, however as RB51 does not have cutoff points, i.e.…”

Section: Bovine Brucellosis Control and Eradication Programsmentioning

confidence: 99%

Brucella Abortus Vaccines: Use in Control Programs and Immune Response

Lage¹

2017

J Bacteriol Mycol

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Brucella spp. is a highly infectious pathogen that affects numerous livestock and wild animal species besides humans, and that although eradicated in some countries, remains one of the most economically important zoonosis worldwide. Strain 19 and RB51 are the two Brucella abortus vaccine strains more largely used in the control of brucellosis in cattle worldwide, being effective in the prevention of abortion and infection, besides offering long lasting protection. Here, we reviewed the main aspects of the B. abortus vaccines and their use in brucellosis control and eradication programs over the years and the current understanding in the immune response triggered by these two B. abortus vaccines, especially in cattle.

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Evaluation of Brucella abortus S19 vaccines commercialized in Brazil: Immunogenicity, residual virulence and MLVA15 genotyping

Cited by 15 publications

References 13 publications

Genetic stability of Brucella abortus isolates from an outbreak by multiple-locus variable-number tandem repeat analysis (MLVA16)

Genetic stability of Brucella abortus isolates from an outbreak by multiple-locus variable-number tandem repeat analysis (MLVA16)

Genetic diversity of Brucella ovis isolates from Rio Grande do Sul, Brazil, by MLVA16

Brucella Abortus Vaccines: Use in Control Programs and Immune Response

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