Convergence of FPR-rs3-expressing neurons in the mouse accessory olfactory bulb

Dietschi, Quentin; Assens, Alexis; Challet, Ludivine; Carleton, Alan; Rodríguez, Iván

doi:10.1016/j.mcn.2013.04.008

Cited by 11 publications

(11 citation statements)

References 43 publications

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“…We never observed colabeling of transgene-expressing and V2R2-immunopositive VSNs ( n = 79) confirming layer-specific expression of the FPR-rs3 transgene in apical VSNs. Immunostaining with an anti-FPR-rs3 antibody (Rivière et al, 2009; Dietschi et al, 2013) revealed 424 out of 53,284 FPR-rs3 + VSNs (0.79%), an expression level within the previously reported range between 0.4% and 0.8% (Rivière et al, 2009; Dietschi et al, 2013). Moreover, all transgene-positive cells ( n = 225) also express the FPR-rs3 protein (Figures 1F–H).…”

Section: Resultssupporting

confidence: 65%

“…Vomeronasal transgene expression in Fpr-rs3-i-Venus mice faithfully recapitulates the punctate apical expression pattern of endogenous FPR-rs3 (Rivière et al, 2009; Dietschi et al, 2013). Furthermore, bicistronic expression of the tau-Venus fusion protein additionally targets the fluorescent marker to axons and axon terminals in the AOB.…”

Section: Discussionmentioning

confidence: 64%

“…V2R neurons predominantly detect peptides/small proteins (Leinders-Zufall et al, 2004; Chamero et al, 2007; Kimoto et al, 2007; Ferrero et al, 2013; Kaur et al, 2014) and project to the posterior region of the AOB. For FPR-rs3 expressing neurons, we recently described axonal projections to the rostral AOB (Dietschi et al, 2013), the target region of V1R neurons.…”

Section: Introductionmentioning

confidence: 99%

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Physiological characterization of formyl peptide receptor expressing cells in the mouse vomeronasal organ

et al. 2014

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The mouse vomeronasal organ (VNO) is a chemosensory structure that detects both hetero- and conspecific social cues. Based on largely monogenic expression of either type 1 or 2 vomeronasal receptors (V1Rs/V2Rs) or members of the formyl peptide receptor (FPR) family, the vomeronasal sensory epithelium harbors at least three neuronal subpopulations. While various neurophysiological properties of both V1R- and V2R-expressing neurons have been described using genetically engineered mouse models, the basic biophysical characteristics of the more recently identified FPR-expressing vomeronasal neurons have not been studied. Here, we employ a transgenic mouse strain that coexpresses an enhanced variant of yellow fluorescent protein together with FPR-rs3 allowing to identify and analyze FPR-rs3-expressing neurons in acute VNO tissue slices. Single neuron electrophysiological recordings allow comparative characterization of the biophysical properties inherent to a prototypical member of the FPR-expressing subpopulation of VNO neurons. In this study, we provide an in-depth analysis of both passive and active membrane properties, including detailed characterization of several types of voltage-activated conductances and action potential discharge patterns, in fluorescently labeled vs. unmarked vomeronasal neurons. Our results reveal striking similarities in the basic (electro) physiological architecture of both transgene-expressing and non-expressing neurons, confirming the suitability of this genetically engineered mouse model for future studies addressing more specialized issues in vomeronasal FPR neurobiology.

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Section: Resultssupporting

confidence: 65%

Section: Discussionmentioning

confidence: 64%

Section: Introductionmentioning

confidence: 99%

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Physiological characterization of formyl peptide receptor expressing cells in the mouse vomeronasal organ

et al. 2014

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“…This is true for V1rs (9, 10), V2rs (11), and Fprs (12). Vomeronasal sensory neurons located in the apical and basal zones do recognize different types of chemicals, mainly kairomones (semiochemicals that mediate interspecific interactions).…”

Section: Significancementioning

confidence: 96%

Evolution of immune chemoreceptors into sensors of the outside world

Dietschi

Tuberosa

Rösingh

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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Changes in gene expression patterns represent an essential source of evolutionary innovation. A striking case of neofunctionalization is the acquisition of neuronal specificity by immune formyl peptide receptors (Fprs). In mammals, Fprs are expressed by immune cells, where they detect pathogenic and inflammatory chemical cues. In rodents, these receptors are also expressed by sensory neurons of the vomeronasal organ, an olfactory structure mediating innate avoidance behaviors. Here we show that two gene shuffling events led to two independent acquisitions of neuronal specificity by Fprs. The first event targeted the promoter of a V1R receptor gene. This was followed some 30 million years later by a second genomic accident targeting the promoter of a V2R gene. Finally, we show that expression of a vomeronasal Fpr can reverse back to the immune system under inflammatory conditions via the production of an intergenic transcript linking neuronal and immune Fpr genes. Thus, three hijackings of regulatory elements are sufficient to explain all aspects of the complex expression patterns acquired by a receptor family that switched from sensing pathogens inside the organism to sensing the outside world through the nose.olfaction | vomeronasal organ | olfactory receptor | gene evolution | neofunctionalization

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“…VNO-expressed receptors are instructive in VSN connectivity to the accessory olfactory bulb (AOB) (Rodriguez et al 1999, Dietschi et al 2013), and we therefore consider receptor choice to be central to VSN identity. Below, we review current understanding of VR expression and gene regulation.…”

Section: The Vomeronasal Organ: Vomeronasal Receptorsmentioning

confidence: 99%

Chemosensory Receptor Specificity and Regulation

Dalton

Lomvardas

2015

Annu. Rev. Neurosci.

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The senses provide a means by which data on the physical and chemical properties of the environment may be collected and meaningfully interpreted. Sensation begins at the periphery, where a multitude of different sensory cell types are activated by environmental stimuli as different as photons and odorant molecules. Stimulus sensitivity is due to expression of different cell surface sensory receptors, and therefore the receptive field of each sense is defined by the aggregate of expressed receptors in each sensory tissue. Here, we review current understanding on patterns of expression and modes of regulation of sensory receptors.

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Convergence of FPR-rs3-expressing neurons in the mouse accessory olfactory bulb

Cited by 11 publications

References 43 publications

Physiological characterization of formyl peptide receptor expressing cells in the mouse vomeronasal organ

Physiological characterization of formyl peptide receptor expressing cells in the mouse vomeronasal organ

Evolution of immune chemoreceptors into sensors of the outside world

Chemosensory Receptor Specificity and Regulation

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