AP-2α suppresses invasion in BeWo cells by repression of matrix metalloproteinase-2 and -9 and up-regulation of E-cadherin

Zhang, Zhan; Zhang, Ling; Jia, Liting; Cui, Shihong; Shi, Ying; Chang, Ana M.; Zeng, Xiaomao; Wang, Peng

doi:10.1007/s11010-013-1685-8

Cited by 17 publications

(11 citation statements)

References 32 publications

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“…MMP‐2 is suggested to have a significant role in regulating the vasculature in rat mesenteric arteries . In contrast, studies report decreased MMP‐2 mRNA expression in placentas from women with preeclampsia as compared to control . The increased MMP‐2 levels observed in this study may contribute to enhanced vascular contraction and hypertension via increased production of vasoactive peptides …”

Section: Discussioncontrasting

confidence: 57%

Matrix metalloproteinases‐2 (MMP‐2) and matrix metalloproteinases ‐9 (MMP‐9) are differentially expressed in different regions of normal and preeclampsia placentae

Sahay

Jadhav

Sundrani

et al. 2018

J of Cellular Biochemistry

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Matrix metalloproteinases (MMPs) are involved in the extracellular matrix (ECM) remodeling during human placentation and parturition and have been shown to be associated with oxidative stress. Placental regional changes in oxygen availability and oxidative stress indices may influence regional differences in expression of MMPs. This study examines the protein and mRNA levels of MMP-2 and MMP-9 in different regions of the placenta in normotensive control (NC) women and women with preeclampsia (PE). Fifty-two NC women and 43 women with PE (18 delivered at term [T-PE] and 25 delivered preterm [PT-PE]) were recruited. Placental samples were taken from four regions: central basal (CM), central chorionic (CF), peripheral basal (PM), and peripheral chorionic (PF). MMP protein and mRNA levels were measured by ELISA and quantitative real time PCR, respectively. MMP-2 protein levels were higher in all the placental regions (P < 0.05) from PT-PE group as compared to the respective regions from the NC and T-PE groups. MMP-9 mRNA levels were higher in CM region as compared to CF and PM regions (P < 0.05) in the NC group and compared to CF and PF regions (P < 0.05) in the T-PE group. The MMP-9 mRNA levels were lower in the CF region in the PT-PE and T-PE groups (P < 0.05) as compared to the NC group. Elevated levels of MMP-2 protein levels were observed in all regions of PT-PE placenta possibly influencing the degradation of placental ECM. Lower mRNA expression of MMP-9 both in PT-PE and T-PE may contribute to a disturbed placental vascularization.

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Section: Discussioncontrasting

confidence: 57%

Matrix metalloproteinases‐2 (MMP‐2) and matrix metalloproteinases ‐9 (MMP‐9) are differentially expressed in different regions of normal and preeclampsia placentae

Sahay

Jadhav

Sundrani

et al. 2018

J of Cellular Biochemistry

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“…Campillos M et al showed that oncogene, DEK, coordinated with AP-2α for the regulation of cell cycle [ 27 ]. Zhang et al reported that overexpression of AP-2α decreased the invasive abilities of BeWo cells by the induction of TIMP-2 and E-cadherin and a significant reduction of MMP-2 and MMP-9 in preeclampsia [ 28 ]. In this study, it was observed that AP-2α could directly bind to the promoter motif of TGFB1 in order to induce TGF-β expression, which downregulated miR-200b expression.…”

Section: Discussionmentioning

confidence: 99%

Role of AP-2α and MAPK7 in the regulation of autocrine TGF-β/miR-200b signals to maintain epithelial-mesenchymal transition in cholangiocarcinoma

Zhang

Jiang

et al. 2017

J Hematol Oncol

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BackgroundCholangiocarcinoma (CCA) is characterized by early lymphatic, metastasis, and low survival rate. Epithelial-mesenchymal transition (EMT) is able to induce tumor metastasis. Although the TGF-β/miR-200 signals promote EMT in various types of cancer, the regulatory mechanism in CCA is still unclear.MethodsExpression of miR-200b, TGF-β, and EMT markers were measured in tumor samples and cell lines by qRT-PCR and western blot. CCK8 assay was performed to measure the cell viability. Transwell assay was used to evaluate migration and invasion. The target genes of miR-200b and transcription factor of TGF-β were analyzed using dual-luciferase reporter system.ResultsWe have demonstrated that CCA exhibited remarkable EMT phenotype and miR-200b was reduced in CCA patients (n = 20) and negatively correlated to TGF-β. Moreover, two CCA cells, HCCC, and RBE, with epithelial appearances treated with TGF-β, showed fibroblastic-like cell morphology with downregulated miR-200b expression. Forced expression of miR-200b abrogated TGF-β-induced EMT initiation, with decreased cell proliferation, migration, and invasion in vitro. Also, TFAP2A (encode AP-2α) and MAPK7 were found to be targeted by miR-200b to downregulate EMT and AP-2α inhibited miR-200b by directly promoting transcription of TGFB1. Overexpression of MAPK7 significantly reversed miR-200b-induced inhibition of EMT, migration, and proliferation by increasing the expression of TGF-β, cyclin D1, and Cdk2. Further, the administration of miR-200b induced a remarkably tumor regression in vivo and reduced the effect of TGF-β-related EMT in AP-2α and MAPK7-dependent manner.ConclusionsOur study highlights that miR-200b-based gene therapy is effective in the treatment of CCA.

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“…On the other hand, this finding could suggest the downregulation of endometrial E-cadherin related to aging. Animal 29 and in vitro 48 studies suggest that E-cadherin is involved in embryo implantation due to its decreasing expressions and activities of MMP-2 and MMP-9. But, inversely to those studies, we found that MMP-2 and MMP-9 were positively correlated with E-cadherin.…”

Section: Discussionmentioning

confidence: 99%

Matrix Metalloproteinases 2 and 9 and E-Cadherin Expression in the Endometrium During the Implantation Window of Infertile Women Before In Vitro Fertilization Treatment

Maia-Filho

Rocha²,

Ferreira

et al. 2015

Reprod. Sci.

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Objective: To evaluate the expression of endometrial matrix metalloproteinases (MMPs) 2 and 9 and E-cadherin in periimplantation phase of infertile women who have undergone in vitro fertilization (IVF) cycles. Methods: This prospective study included 51 patients who underwent endometrial biopsy during the receptive phase in a menstrual cycle prior to IVF treatment. The samples were evaluated by tissue microarray for immunohistochemical study. Results: The expression of MMP-2, MMP-9, and E-cadherin in the endometrium prior to IVF treatment was not associated with pregnancy. There was a decrease in E-cadherin immunodetection, the higher the age of the patients, a negative relationship between E-cadherin and MMP-2, and a positive association between MMP-9 and E-cadherin. Conclusions: The MMP-2, MMP-9, and E-cadherin are expressed in the endometrium of infertile patients during the receptive phase of the natural menstrual cycle. However, there is no correlation between the expression of these molecules and the clinical IVF outcomes.

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AP-2α suppresses invasion in BeWo cells by repression of matrix metalloproteinase-2 and -9 and up-regulation of E-cadherin

Cited by 17 publications

References 32 publications

Matrix metalloproteinases‐2 (MMP‐2) and matrix metalloproteinases ‐9 (MMP‐9) are differentially expressed in different regions of normal and preeclampsia placentae

Matrix metalloproteinases‐2 (MMP‐2) and matrix metalloproteinases ‐9 (MMP‐9) are differentially expressed in different regions of normal and preeclampsia placentae

Role of AP-2α and MAPK7 in the regulation of autocrine TGF-β/miR-200b signals to maintain epithelial-mesenchymal transition in cholangiocarcinoma

Matrix Metalloproteinases 2 and 9 and E-Cadherin Expression in the Endometrium During the Implantation Window of Infertile Women Before In Vitro Fertilization Treatment

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