Nanomechanical analysis of pigmented human melanoma cells

Sarna, Michał; Piłat, Anna; Olchawa, Magdalena; Gkogkolou, Paraskevi; Burda, Květoslava; Böhm, Markus; Sarna, Tadeusz

doi:10.1111/pcmr.12113

Cited by 27 publications

(37 citation statements)

References 14 publications

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“…Moreover, the log‐normal distribution of Young's modulus values for pigmented cells becomes more pronounced as the number of melanosomes inside cells increases. Similar effect was observed in our previous studies and discussed in detail. Numerical values of Young's modulus together with the number of melanin granules inside cells are shown in Table .…”

Section: Resultssupporting

confidence: 92%

Cell elasticity is an important indicator of the metastatic phenotype of melanoma cells

Sarna

Żądło

Hermanowicz

et al. 2014

Experimental Dermatology

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The relationship between melanin pigmentation and metastatic phenotype of melanoma cells is an intricate issue, which needs to be unambiguously determined to fully understand the process of metastasis of malignant melanoma. Despite significant research efforts undertaken to solve this problem, the outcomes are far from being satisfying. Importantly, none of the proposed explanations takes into consideration biophysical aspects of the phenomenon such as cell elasticity. Recently, we have demonstrated that melanin granules dramatically modify elastic properties of pigmented melanoma cells. This prompted us to examine the mechanical effects of melanosomes on the transmigration abilities of melanoma cells. Here, we show for the first time that melanin granules inhibit transmigration abilities of melanoma cells in a number of granules dependent manner. Moreover, we demonstrate that the inhibitory effect of melanosomes is mechanical in nature. Results obtained in this study demonstrate that cell elasticity may play a key role in the efficiency of melanoma cells spread in vivo. Our findings may also contribute to better understanding of the process of metastasis of malignant melanoma.

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Section: Resultssupporting

confidence: 92%

Cell elasticity is an important indicator of the metastatic phenotype of melanoma cells

Sarna

Żądło

Hermanowicz

et al. 2014

Experimental Dermatology

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“…A variety of techniques have been used to study cancer cell stiffness including: micropipette aspiration (Ward et al 1991), optical deformity (Guck et al 2005), particle tracking microrheology (Baker et al 2010), and observation of cell bound magnetic beads (Swaminathan et al 2011; Coughlin et al 2013). Atomic force microscopy (AFM) permits direct physical measurement of the cell surface, and has confirmed the low stiffness of both isolated cancer cells (Lekka et al 1999; Cross et al 2007; Cross et al 2008; Fuhrmann et al 2011; Xu et al 2012; Sarna et al 2013; Efremov et al 2014; Ramos et al 2014; Weder et al 2014), and cancer cells in tissues (Plodinec et al 2012). …”

Section: Introductionmentioning

confidence: 99%

“…The relationship between malignant behavior and cell-stiffness is, however, not simple, such that for example, the presence of melanosomes in melanoma cells correlates with increased cell stiffness (Sarna, Zadlo et al 2013). Further related to this, is that despite initial reduction in cell-stiffness with melanoma progression, further metastasis is associated with a relative increase in cell-stiffness (Weder, Hendriks-Balk et al 2014).…”

Section: Introductionmentioning

confidence: 99%

A novel cell-stiffness-fingerprinting analysis by scanning atomic force microscopy: comparison of fibroblasts and diverse cancer cell lines

Zoellner

Paknejad

Manova

et al. 2015

Histochem Cell Biol

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Differing stimuli affect cell-stiffness while cancer metastasis further relates to cell-stiffness. Cell-stiffness determined by atomic Force Microscopy (AFM) has been limited by measurement over nuclei to avoid spurious substratum effects in thin cytoplasmic domains, and we sought to develop a more complete approach including cytoplasmic areas. 90 μm square fields were recorded from 10 sites of cultured Human Dermal Fibroblasts (HDF), and 3 sites each for melanoma (MM39, WM175, MeIRMu), osteosarcoma (SAOS-2, U2OS), and ovarian carcinoma (COLO316, PEO4) cell lines, each site providing 1,024 measurements as 32x32 square grids. Stiffness recorded below 0.8 μm height was occasionally influenced by substratum, so only stiffness recorded above 0.8 μm was analyzed, but all sites were included for height and volume analysis. COLO316 had the lowest cell height and volume, followed by HDF (p<0.0001), and then PEO4, SAOS-2, MeIRMu, WM175, U2OS, and MM39. HDF were more stiff than all other cells (p < 0.0001), while in descending order of stiffness were PEO4, COLO316, WM175, SAOS-2, U2OS, MM39, and MeIRMu (p < 0.02). Stiffness-fingerprints comprised scattergrams of stiffness values plotted against the height at which each stiffness value was recorded, and appeared unique for each cell type studied, although in most cases the overall form of fingerprints was similar, with maximum stiffness at low height measurements and a second lower peak occurring at high height levels. We suggest our stiffness-fingerprint analytical method provides a more nuanced description than previously reported, and will facilitate study of the stiffness response to cell stimulation.

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“…Furthermore, changes of keratin phosphorylation and reorganization seem to be related with physical properties of cells, including viscoelasticity which is believed to be a key parameter of metastasis of cancer cells (Beil et al, ; Busch et al, ). The clinical importance of cell softness and changes of viscoelasticity, has been reported by several researchers (Cross et al, ; Sarna et al, ).…”

Section: Introductionmentioning

confidence: 86%

Effects of cerulein on keratin 8 phosphorylation and perinuclear reorganization in pancreatic cancer cells: Involvement of downregulation of protein phosphatase 2A and alpha4

Park

Lee

2015

Environ. Toxicol.

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Toxicants can perturb cellular homeostasis by modifying phosphorylation-based signaling. In the present study, we examined the effects of cerulein, an inducer of acute pancreatitis, on keratin 8 (K8) phosphorylation. We found that cerulein dose-dependently induced K8 phosphorylation and perinuclear reorganization in PANC-1 cells, thus leading to migration and invasion. The extracellular signal-regulated kinases (ERK) inhibitor U0126 suppressed cerulein-induced phosphorylation of serine 431 and reorganization of K8. Cerulein reduced the expressions of protein phosphatase 2A (PP2A) via ubiqutination and alpha4. PP2A's involvement in K8 phosphorylation of PANC-1 cells was also confirmed by the observation that PP2A gene silencing resulted in K8 phosphorylation and migration of PANC-1 cells. Overall, these results suggest that cerulein induced phosphorylation and reorganization through ERK activation by downregulating PP2A and alpha4, leading to increased migration and invasion of PANC-1 cells. © 2015 Wiley Periodicals, Inc. Environ Toxicol 31: 2090-2098, 2016.

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Nanomechanical analysis of pigmented human melanoma cells

Cited by 27 publications

References 14 publications

Cell elasticity is an important indicator of the metastatic phenotype of melanoma cells

Cell elasticity is an important indicator of the metastatic phenotype of melanoma cells

A novel cell-stiffness-fingerprinting analysis by scanning atomic force microscopy: comparison of fibroblasts and diverse cancer cell lines

Effects of cerulein on keratin 8 phosphorylation and perinuclear reorganization in pancreatic cancer cells: Involvement of downregulation of protein phosphatase 2A and alpha4

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