Reactive oxygen species (ROS) production in human peripheral blood neutrophils exposed<i>in vitro</i>to static magnetic field

Poniedziałek, Barbara; Rzymski, Piotr; Karczewski, Jacek; Jaroszyk, Feliks; Wiktorowicz, Krzysztof

doi:10.3109/15368378.2013.773910

Cited by 19 publications

(13 citation statements)

References 14 publications

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“…Goraca and colleagues [ 22 ] reported a decrease in antioxidant capacity in hearts of rats subjected to chronic LF-EMF exposure. Similarly, prolonged exposure to LF-EMF enhanced free radical generation in the brain and retina of rats leading to the reduced antioxidant defense capacity and increased lipid peroxidation [ 23 – 25 ]. Recently, it has been shown that the exposure of blood samples to LF-EMF induced changes in ROS production in both stimulated and nonstimulated neutrophils [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

Low Frequency Electromagnetic Field Conditioning Protects against I/R Injury and Contractile Dysfunction in the Isolated Rat Heart

Biały

Wawrzyńska

Bil‐Lula

et al. 2015

BioMed Research International

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Low frequency electromagnetic field (LF-EMF) decreases the formation of reactive oxygen species, which are key mediators of ischemia/reperfusion (I/R) injury. Therefore, we hypothesized that the LF-EMF protects contractility of hearts subjected to I/R injury. Isolated rat hearts were subjected to 20 min of global no-flow ischemia, followed by 30 min reperfusion, in the presence or absence of LF-EMF. Coronary flow, heart rate, left ventricular developed pressure (LVDP), and rate pressure product (RPP) were determined for evaluation of heart mechanical function. The activity of cardiac matrix metalloproteinase-2 (MMP-2) and the contents of coronary effluent troponin I (TnI) and interleukin-6 (IL-6) were measured as markers of heart injury. LF-EMF prevented decreased RPP in I/R hearts, while having no effect on coronary flow. In addition, hearts subjected to I/R exhibited significantly increased LVDP when subjected to LF-EMF. Although TnI and IL-6 levels were increased in I/R hearts, their levels returned to baseline aerobic levels in I/R hearts subjected to LF-EMF. The reduced activity of MMP-2 in I/R hearts was reversed in hearts subjected to LF-EMF. The data presented here indicate that acute exposure to LF-EMF protects mechanical function of I/R hearts and reduces I/R injury.

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Section: Discussionmentioning

confidence: 99%

Low Frequency Electromagnetic Field Conditioning Protects against I/R Injury and Contractile Dysfunction in the Isolated Rat Heart

Biały

Wawrzyńska

Bil‐Lula

et al. 2015

BioMed Research International

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“…In addition, Poniedzialek et al also showed that in human peripheral blood neutrophils, ROS level was significantly reduced after 60 mT SMF exposed for 15 min, but recovered to control level at 30 min. More surprisingly, ROS level was later visibly elevated at 45 min, but only in the cells that were exposed to the south poles of the magnets, not north poles [ 30 ], indicating that the effects of SMFs on cellular ROS were dependent on both exposure time and the magnetic poles. It could be argued that these effects are likely due to MF direction, rather than the magnetic poles themselves.…”

Section: The Effects Of Magnetic Fields On Rosmentioning

confidence: 99%

Magnetic Fields and Reactive Oxygen Species

Wang

Zhang

2017

IJMS

174

138

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Reactive oxygen species (ROS) ubiquitously exist in mammalian cells to participate in various cellular signaling pathways. The intracellular ROS levels are dependent on the dynamic balance between ROS generation and elimination. In this review, we summarize reported studies about the influences of magnetic fields (MFs) on ROS levels. Although in most cases, MFs increased ROS levels in human, mouse, rat cells, and tissues, there are also studies showing that ROS levels were decreased or not affected by MFs. Multiple factors could cause these discrepancies, including but not limited to MF type/intensity/frequency, exposure time and assay time-point, as well as different biological samples examined. It will be necessary to investigate the influences of different MFs on ROS in various biological samples systematically and mechanistically, which will be helpful for people to get a more complete understanding about MF-induced biological effects. In addition, reviewing the roles of MFs in ROS modulation may open up new scenarios of MF application, which could be further and more widely adopted into clinical applications, particularly in diseases that ROS have documented pathophysiological roles.

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“…Furthermore, in a recent study a significant decline in ROS production by human peripheral blood neutrophils has been shown after 15 min exposure to SMF (approx. 60 mT), while a longer incubation time (45 min) caused a reverse phenomenon [41]. The authors conclude that the SMF-exposure may directly modulate the activity of neutrophil NADPH oxidases and they highlight the importance of proper adjustment of exposure time to SMF for any potential therapeutic applications.…”

Section: Discussionmentioning

confidence: 89%

Exposure to inhomogeneous static magnetic field beneficially affects allergic inflammation in a murine model

Csillag

Kumar

Szabó

et al. 2014

J. R. Soc. Interface.

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Previous observations suggest that static magnetic field (SMF)-exposure acts on living organisms partly through reactive oxygen species (ROS) reactions. In this study, we aimed to define the impact of SMF-exposure on ragweed pollen extract (RWPE)-induced allergic inflammation closely associated with oxidative stress. Inhomogeneous SMF was generated with an apparatus validated previously providing a peak-to-peak magnetic induction of the dominant SMF component 389 mT by 39 T m 21 lateral gradient in the in vivo and in vitro experiments, and 192 mT by 19 T m 21 in the human study at the 3 mm target distance. Effects of SMF-exposure were studied in a murine model of allergic inflammation and also in human provoked skin allergy. We found that even a single 30-min exposure of mice to SMF immediately following intranasal RWPE challenge significantly lowered the increase in the total antioxidant capacity of the airways and decreased allergic inflammation. Repeated (on 3 consecutive days) or prolonged (60 min) exposure to SMF after RWPE challenge decreased the severity of allergic responses more efficiently than a single 30-min treatment. SMF-exposure did not alter ROS production by RWPE under cell-free conditions, while diminished RWPEinduced increase in the ROS levels in A549 epithelial cells. Results of the human skin prick tests indicated that SMF-exposure had no significant direct effect on provoked mast cell degranulation. The observed beneficial effects of SMF are likely owing to the mobilization of cellular ROS-eliminating mechanisms rather than direct modulation of ROS production by pollen NAD(P)H oxidases.

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Reactive oxygen species (ROS) production in human peripheral blood neutrophils exposedin vitroto static magnetic field

Cited by 19 publications

References 14 publications

Low Frequency Electromagnetic Field Conditioning Protects against I/R Injury and Contractile Dysfunction in the Isolated Rat Heart

Low Frequency Electromagnetic Field Conditioning Protects against I/R Injury and Contractile Dysfunction in the Isolated Rat Heart

Magnetic Fields and Reactive Oxygen Species

Exposure to inhomogeneous static magnetic field beneficially affects allergic inflammation in a murine model

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