Identification of a subpopulation of nasopharyngeal carcinoma cells with cancer stem‐like cell properties by high aldehyde dehydrogenase activity

Yu, F.; Sim, Adrian Chong Nyi; Li, Chunwei; Li, Yingying; Zhao, Xuening; Wang, Deyun; Loh, Kwok Seng

doi:10.1002/lary.24003

Cited by 27 publications

(20 citation statements)

References 30 publications

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“…After decanting with Amp4 solution, it was then washed twice in deionized water to proceed to IHC. All steps of IHC were performed as previously described except for the primary antibody incubation. The incubation time at 4°C varied from 24 to 72 hours based on different antibodies.…”

Section: Methodsmentioning

confidence: 99%

Presence of lytic Epstein‐Barr virus infection in nasopharyngeal carcinoma

Petersson

et al. 2018

Head & Neck

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Section: Methodsmentioning

confidence: 99%

Presence of lytic Epstein‐Barr virus infection in nasopharyngeal carcinoma

Petersson

et al. 2018

Head & Neck

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“…These cells have high proliferation rate and differentiation ability, strong colony/sphere formation and migration ability compared with negative counterparts. Multivariate analysis indicates that ALDH1 could be an independent prognostic marker for NPC [13, 14]. Cell surface marker staining is another reliable method for CSC isolation.…”

Section: Introductionmentioning

confidence: 99%

E3-ligase Skp2 predicts poor prognosis and maintains cancer stem cell pool in nasopharyngeal carcinoma

et al. 2014

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Nasopharyngeal carcinoma (NPC) is one of the severe head and neck carcinomas, which is rare in west countries but has high incidence in Southern Asia especially South China. Although NPC is relatively sensitive to radiotherapy, the prognosis of patients is poor due to the advanced stage at the time of diagnosis. Therefore, it is important to understand the mechanisms involved in tumorigenesis and develop early diagnostic techniques. S-phase kinase associated protein 2 (Skp2) is overexpressed in several human cancers and associates with poor prognosis. However, its function in NPC has not been fully addressed. In this study we found Skp2 was highly expressed in NPC specimen and correlated with poor prognosis. We generated Skp2 knockdown cells to further delineate its role in NPC development. Knockdown of Skp2 partially reduced cell proliferation, promoted cellular senescence, and decreased the population of stem cell like aldehyde dehydrogenase1 positive cells as well as their self-renewal ability. Our study not only interprets the predictive role of Skp2 in the poor prognosis of NPC patients, but also reveals that Skp2 regulates the NPC cancer stem cell maintenance, which shed lights on the target therapy and early diagnosis of NPC in clinical application.

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“…These findings were followed by detection of CSCs in clinical samples that expressed OCT4, SOX2, CD133, NESTIN, and MCM2 [63]. Despite contradictive reports on the role of ABCG2 [62,64] and CD133 [65,66] as NPC-SC markers, CD44 has been suggested as a suitable molecule; CD44 + cells from NPC cell lines not only expressed BMI1, OCT4 [67], and SOX2 [68] but also were highly proliferative, chemoresistant, and tumorigenic [67][68][69]. In addition, clinical studies revealed a relation between CD44 expression and tumor grade and prognosis in pharyngeal cancer [70].…”

Section: Pharynxmentioning

confidence: 96%

“…It has also been suggested that CD24 could be considered as a marker for nasopharyngeal CSCs, since CD24 + cells from NPC cell lines expressed SC genes and were highly proliferative, tumorigenic, and drug resistant [71]. The other introduced molecule for NPC-SCs is ALDH1, because cells with ALDH1 activity expressed pluripotent markers and presented great proliferation, differentiation, migration, and tumorigenic abilities [65,72,73]. Moreover, clinical studies indicated that ALDH1 expression was associated with aggressive tumor behavior and poor survival of NPC [74].…”

Section: Pharynxmentioning

confidence: 97%

Cancer stem cells in human digestive tract malignancies

2015

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Digestive tract malignancies, including oral, pharyngeal, esophageal, gastric, and colorectal cancers, are among the top 10 most common cancers worldwide. In spite of using various treatment modalities, cancer patients still suffer from recurrence and metastasis of malignant cells. Cancer stem cells (CSCs) are undifferentiated and highly proliferative malignant cells with unique properties mediated by overexpression of stemness markers, metastasis-related proteins, drug transporters, and DNA repair machinery. Due to their salient characteristics, it has been suggested that CSCs are responsible for tumor initiation, progression, invasion, recurrence, and therapy resistance. Exploring different aspects of CSC biology has fueled a great enthusiasm in designing novel therapeutic strategies to help patients. For instance, identification of markers associated with digestive tract CSCs, such as CD44, CD133, CD24, EpCAM, LGR5, ALDH1, and BMI1, has made it possible to develop more accurate diagnosis approaches. In addition, specifically targeting CSCs by their markers imposes fewer side effects and improves therapeutic outcomes. Here, we focus on the current status of CSC biology in digestive tract cancers, with emphasis on CSC markers, and review achieved progress in eradication of digestive tract CSC cells.

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Identification of a subpopulation of nasopharyngeal carcinoma cells with cancer stem‐like cell properties by high aldehyde dehydrogenase activity

Abstract: These results provide evidence for the existence of cancer stem-like cells in the NPC cell line C666-1 cells.

Cited by 27 publications

References 30 publications

Presence of lytic Epstein‐Barr virus infection in nasopharyngeal carcinoma

Presence of lytic Epstein‐Barr virus infection in nasopharyngeal carcinoma

E3-ligase Skp2 predicts poor prognosis and maintains cancer stem cell pool in nasopharyngeal carcinoma

Cancer stem cells in human digestive tract malignancies

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