Anti-inflammatory BmAFI of Brugia malayi modulates IgE, histamine and histamine receptor responses in Mastomys coucha

Joseph, Sujith; Verma, Shiv Kumar; Verma, Richa; Saxena, Jitendra Kumar; Srivastava, Mukesh; Murthy, P. K.

doi:10.1016/j.actatropica.2013.04.006

Cited by 3 publications

(2 citation statements)

References 30 publications

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“…While basophilic granulocytes are often studied in M. coucha during filarial infections [25,26], our study of healthy helminth-free M. natalensis did not allow us to document the characteristics of this rarer cell population. Comparing M. natalensis to other typical laboratory rodents, the measured leukocyte concentrations are much closer to reference ranges of the highly standardized C57BL/6 mouse strain, matching their closer genetic relation to mice [3], than to those of rats.…”

Section: Discussionmentioning

confidence: 90%

Hematology and Clinical Chemistry Reference Ranges for Laboratory-Bred Natal Multimammate Mice (Mastomys natalensis)

Wozniak

Kirchoff

Hansen-Kant

et al. 2021

Viruses

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Laboratory-controlled physiological data for the multimammate rat (Mastomys natalensis) are scarce, despite this species being a known reservoir and vector for zoonotic viruses, including the highly pathogenic Lassa virus, as well as other arenaviruses and many species of bacteria. For this reason, M. natalensis is an important rodent for the study of host-virus interactions within laboratory settings. Herein, we provide basic blood parameters for age- and sex-distributed animals in regards to blood counts, cell phenotypes and serum chemistry of a specific-pathogen-monitored M.natalensis breeding colony, to facilitate scientific insight into this important and widespread rodent species.

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Section: Discussionmentioning

confidence: 90%

Hematology and Clinical Chemistry Reference Ranges for Laboratory-Bred Natal Multimammate Mice (Mastomys natalensis)

Wozniak

Kirchoff

Hansen-Kant

et al. 2021

Viruses

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“…Recently, Kalesnikoff and Galli (2011) have shown that such positive and negative im munomodulation may be mediated at least in part by histamine. Joseph et al (2013) have also shown that histamine plays an important role in facilitating infection in animals sensitized with the anti-inflammatory BmAF1 fraction. The mechanism by which mast cell products facilitated parasite survival in the present study is, however, not clear.…”

Section: Discussionmentioning

confidence: 94%

Modulation of host’s immune responses and parasite load in Mastomys coucha immunized with recombinant mitochondrial HSP60 of human lymphatic filarial parasite Brugia malayi

Verma

Kushwaha

et al. 2013

J. Exper. Appl. Anim. Sci.

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Recently we have shown that mitochondrial HSP60 of Brugia malayi (mtHSP60bm) shows high degree of homology with Escherichia coli GroEL/ES and that the ATP binding pockets of HSP60 in humans, E. coli and B. malayi were structurally conserved. In the present study we investigated the immune responses to rmtHSP60bm in Mastomys coucha and the fate of infection in the immunized animals. The animals received 4 immunization doses of rmtHSP60bm and were subsequently exposed to B. malayi infection. Microfilaremia, adult worm status, nitric oxide (NO), Th1 (IFN-g, IL-2) and Th2 (IL-4, IL 10 and TGF-β) cytokine release, cell proliferative response, levels of specific IgG and its subclasses, and the mast cell status in lymph nodes were assessed on day 135/136 post infection. Immunization with rmtHSP60bm and subsequent exposure to infection resulted in significantly high microfilaraemia but without any change in adult worm burden. Immunization with rmtHSP60bm increased IgG, IgG1 and IgG2b levels, and IL-2 and IFN-g release and suppressed NO release and CMI responses, but without any change in IL-4 and IL-10 release. Exposure of immunized animals to infection enhanced the CMI responses and, NO and IL- 10 release but decreased IgG1 levels and IL-2 and IL-4 release; however, IgG, IgG2a, IgG2b, and IFN-g responses remained unaltered. Mast cells in the draining lymph nodes of immunized-infected animals showed significant degranulation but without any increase in cell count. However, no pathology was found in the lymph nodes. These findings indicate that mtHSP60bm may modulate and balance the host’s immune responses to favor parasite survival without inducing any pathology.

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Strategies to Control Human Lymphatic Filarial Infection: Tweaking Host’s Immune System

Murthy

2019

CTMC

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Human lymphatic filariasis (LF), a parasitic infection caused by the nematodes Wuchereria bancrofti, Brugia malayi and B. timori, and transmitted by mosquito, results in a debilitating disease commonly identified as ‘elephantiasis’. LF affects millions of people in India and several other tropical and sub-tropical countries imposing a huge economic burden on governments due to disability associated loss of man-hours and for disease management. Efforts to control the infection by WHO’s mass drug administration (MDA) strategy using three antifilarials diethylcarbamazine, albendazole and ivermectin are only partly successful and therefore, there is an immediate need for alternative strategies. Some of the alternative strategies being explored in laboratories are: enhancing the immune competence of host by immunomodulation, combining immunomodulation with antifilarials, identifying immunoprophylactic parasite molecules (vaccine candidates) and identifying parasite molecules that can be potential drug targets. This review focuses on the advances made in this direction.

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Anti-inflammatory BmAFI of Brugia malayi modulates IgE, histamine and histamine receptor responses in Mastomys coucha

Cited by 3 publications

References 30 publications

Hematology and Clinical Chemistry Reference Ranges for Laboratory-Bred Natal Multimammate Mice (Mastomys natalensis)

Hematology and Clinical Chemistry Reference Ranges for Laboratory-Bred Natal Multimammate Mice (Mastomys natalensis)

Modulation of host’s immune responses and parasite load in Mastomys coucha immunized with recombinant mitochondrial HSP60 of human lymphatic filarial parasite Brugia malayi

Strategies to Control Human Lymphatic Filarial Infection: Tweaking Host’s Immune System

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