Technologies for deriving primary tumor cells for use in personalized cancer therapy

Mitra, Abhisek; Mishra, Lopa; Li, Shulin

doi:10.1016/j.tibtech.2013.03.006

Cited by 165 publications

(163 citation statements)

References 70 publications

(66 reference statements)

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“…miR-20a, miR-20b, and miR-367 only enhanced drug response in SNU-739 cells (Supplementary information, Figure S2A-S2L), and miR-183 exhibited no sensitizing effect in these cells lines (Supplementary information, Figure S2A-S2L). To further characterize the function of these miRNAs, we analyzed the drug sensitizing effect on primary cultured human HCC cells, which retained the properties of original cancers [24]. Remarkably, miR27b significantly enhanced the response of liver cancer cells to doxorubicin in three out of five patient-derived primary cultures ( Figure 2B).…”

Section: Mir-27b Is a Master Mirna Capable Of Enhancing Drug Sensitivmentioning

confidence: 99%

miR-27b synergizes with anticancer drugs via p53 activation and CYP1B1 suppression

Zhang

et al. 2015

Cell Res

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Liver and kidney cancers are notorious for drug resistance. Due to the complexity, redundancy and interpatient heterogeneity of resistance mechanisms, most efforts targeting a single pathway were unsuccessful. Novel personalized therapies targeting multiple essential drug resistance pathways in parallel hold a promise for future cancer treatment. Exploiting the multitarget characteristic of microRNAs (miRNAs), we developed a new therapeutic strategy by the combinational use of miRNA and anticancer drugs to increase drug response. By a systems approach, we identified that miR-27b, a miRNA deleted in liver and kidney cancers, sensitizes cancer cells to a broad spectrum of anticancer drugs in vitro and in vivo. Functionally, miR-27b enhances drug response by activating p53-dependent apoptosis and reducing CYP1B1-mediated drug detoxification. Notably, miR-27b promotes drug response specifically in patients carrying p53-wild-type or CYP1B1-high signature. Together, we propose that miR-27b synergizes with anticancer drugs in a defined subgroup of liver and kidney cancer patients.

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Section: Mir-27b Is a Master Mirna Capable Of Enhancing Drug Sensitivmentioning

confidence: 99%

miR-27b synergizes with anticancer drugs via p53 activation and CYP1B1 suppression

Zhang

et al. 2015

Cell Res

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“…Moreover, the system provides quick access to drug efficacy and presents relevant information to identify potential response as anticancer medicine in the complex microenvironment of tumor tissue (Van der Kuip et al 2006, Mitra et al 2013.…”

Section: Discussionmentioning

confidence: 99%

Immunocytochemical characterization of primary cell culture in canine transmissible venereal tumor

et al. 2016

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RESUMO.-[Caracterização imunocitoquímica de culturas primárias de tumor venéreo transmissível canino.]Os anticorpos anti-vimentina, anti-lisozima, anti-alfa 1 antitripsina, anti-CD3 e anti-CD79α foram empregados para a caracterização de culturas primárias de tumor venéreo transmissível canino (TVT). Amostras para cultura primá-ria e imuno-histoquímica foram coletadas de oito cães com diagnóstico clínico e citológico de TVT. Para validar o resultado inmunocitoquímico nas culturas de TVT foi realizada a contagem de cromossomos. Para a análise estatística o teste de Mann-Whitney foi empregado a um nível de significância de p<0.05. As culturas e os tecidos de TVT apresentaram intensa reatividade para vimentina, moderada a leve para Lisozima, moderada para alfa-antitripsina e não houve marcação para CD3 e CD79α. Immunochemistry with anti-vimentin, anti-lysozyme, anti-alpha 1 antitrypsin, anti-CD3 and anti-CD79α antibodies has been used for characterization of primary cell culture in the transmissible venereal tumor (TVT). Samples for primary cell culture and immunohistochemistry assays were taken from eight dogs with cytological and clinical diagnosis of TVT. To validate the immunochemical results in the primary cell culture of TVT, a chromosome count was performed. For the statistical analysis, the Mann-Whitney test with p<0.05 was used. TVT tissues and culture cells showed intense anti-vimentin immunoreactivity, lightly to moderate immunoreactivity for anti-lysozyme, and mild for anti-alpha-antitrypsin. No marking was achieved for CD3 and CD79α. All culture cells showed chromosomes variable number of 56 to 68. This is the first report on the use of immunocytochemical characterization in cell culture of TVT. Significant statistic difference between immunochemistry in tissue and culture cell was not established, what suggests that the use of this technique may provide greater certainty for the confirmation of tumors in the primary culture. This fact is particularly important because in vitro culture of tumor tissues has been increasingly used to provide quick access to drug efficacy and presents relevant information to identify potential response to anticancer medicine; so it is possible to understand the behavior of the tumor. Immunocytochemical characterization of primary cell culture in transmissible venereal tumor o uso da immunocitoquímica para a caracterização de culturas de TVT. Assim, e devido ao fato de se observar semelhança entre a imunomarcação em células e tecidos, sugere-se que o uso desta técnica possa auxiliar na confirmação de culturas primárias do tumor, fato muito importante porque a utilização da cultura do tumor pode permitir o acesso a informação relevante sobre resposta potencial a um tratamento e conhecimento do comportamento biológico do tumor.TERMOS DE INDEXAÇÃO: Tumor venéreo transmissível canino, alfa 1 antitripsina, citogenética, lisozima, vimentina.

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“…Accumulation of genetic aberrations in cancer cell lines occurs with increasing number of passages severely limiting their usefulness for personalized medicine [1,2]. Research reviewed here indicates that isolation and culturing of primary tumor cells in 3D is possible and it is likely that primary 3D tumor cultures will soon be routinely used for individualized solid tumor testing.…”

Section: Discussionmentioning

confidence: 99%

“…Accumulation of genetic aberrations in cancer cell lines occurs with increasing number of passages severely limiting their usefulness for personalized medicine [1,2]. In contrast, the critical importance of technologies related to the derivation, short term culture, and testing of primary tumor cells from solid tumors is increasingly recognized (for a recent review see 2).…”

Section: Introductionmentioning

confidence: 99%

An Emerging Role of Biorepositories In Personalized Medicine: Isolation and Processing of Primary Tumor Cells For the Establishment of 3d Tumor Cultures From Solid Tumor Specimens

Valyi‐Nagy¹

2016

Ely J Can Res

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The introduction of three-dimensional (3D) tumor cultures has revolutionized anticancer drug research as these cultures allow for the study of drug resistance mechanisms that cannot be explored in traditional two dimensional (2D) monolayer cultures. Discoveries in the 3D tumor culture field suggest that individualized drug sensitivity testing of solid tumor specimens through the establishment and use of 3D tumor cell cultures following tissue collection will become a routine service offered by modern tissue repositories as they expand from their traditional research role to active participation in personalized medicine. Unfortunately, most information related to 3D tumor cultures comes from studies using established tumor cell lines rather than primary tumor cultures. However, accumulation of genetic aberrations in cancer cell lines occurs with increasing number of passages severely limiting their usefulness for personalized medicine. There is only very limited information available concerning technologies and standard operating procedures for the efficient and routine isolation and processing of primary tumor cells for the establishment of 3D tumor cultures from solid tumor specimens. The purpose of this work was to review experimental data from the literature that may provide relevant information concerning the isolation and processing of primary tumor cells for the establishment of 3D tumor cultures. Information reviewed here may help biorepositories in the development and standardization of technologies and standard operating procedures related to the use of 3D tumor cultures.

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Technologies for deriving primary tumor cells for use in personalized cancer therapy

Cited by 165 publications

References 70 publications

miR-27b synergizes with anticancer drugs via p53 activation and CYP1B1 suppression

miR-27b synergizes with anticancer drugs via p53 activation and CYP1B1 suppression

Immunocytochemical characterization of primary cell culture in canine transmissible venereal tumor

An Emerging Role of Biorepositories In Personalized Medicine: Isolation and Processing of Primary Tumor Cells For the Establishment of 3d Tumor Cultures From Solid Tumor Specimens

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