The in vitro effect of duroquinone on functional competence, genomic integrity, and oxidative stress indices of sterlet (Acipenser ruthenus) spermatozoa

Linhartova, Pavla; Gazo, Ievgeniia; Shaliutina, Anna; Hulák, Martin

doi:10.1016/j.tiv.2013.04.002

Cited by 27 publications

(6 citation statements)

References 38 publications

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“…Most importantly, we observed quinone-like compounds from the vaping of VE and VEA (Figure , Tables S6 and 7, and Figure S2) with 0.89 ± 0.09 μg/mg-diluent of duroquinone (DQ) from VE vaping and 2.45 ± 0.23 μg/mg-diluent of DQ and 12.00 ± 1.10 μg/mg-diluent of durohydroquinone (DHQ) from VEA vaping. Since quinone-like compounds are significant contributors to the generation of reactive oxygen species through redox reaction, they might explain the significant increase of cytotoxicity of VE and VEA vaping samples, acute oxidative stress, and the reported EVALI cases. ,, The presence of DQ and DHQ has been reported in previous studies, , but as far as we know this is the first study to quantify the yield of DQ and DHQ from vaping of VE and VEA. Future studies are warranted to further investigate the role of DQ and DHQ redox couple in cellular oxidative stress and EVALI.…”

Section: Resultsmentioning

confidence: 71%

Chemical and Toxicological Characterization of Vaping Emission Products from Commonly Used Vape Juice Diluents

Jiang

Ahmed

Martin³

et al. 2020

Chem. Res. Toxicol.

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Recent reports have linked severe lung injuries and deaths to the use of e-cigarettes and vaping products. Nevertheless, the causal relationship between exposure to vaping emissions and the observed health outcomes remains to be elucidated. Through chemical and toxicological characterization of vaping emission products, this study demonstrates that during vaping processes, changes in chemical composition of several commonly used vape juice diluents (also known as cutting agents) lead to the formation of toxic byproducts, including quinones, carbonyls, esters, and alkyl alcohols. The resulting vaping emission condensates cause inhibited cell proliferation and enhanced cytotoxicity in human airway epithelial cells. Notably, substantial formation of the duroquinone and durohydroquinone redox couple was observed in the vaping emissions from vitamin E acetate, which may be linked to acute oxidative stress and lung injuries reported by previous studies. These findings provide an improved molecular understanding and highlight the significant role of toxic byproducts in vapingassociated health effects.

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Section: Resultsmentioning

confidence: 71%

Chemical and Toxicological Characterization of Vaping Emission Products from Commonly Used Vape Juice Diluents

Jiang

Ahmed

Martin³

et al. 2020

Chem. Res. Toxicol.

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“…In the current study using spermatozoa of the common carp, excess ROS led to decreases in sperm motility, velocity, and DNA integrity in a time‐ and dose‐dependent manner. We previously showed that, in the presence of various xenobiotic compounds, both spermatozoa motility and DNA integrity are inversely correlated with indicators of oxidative stress such as lipid oxidation and protein carbonylation (Gazo et al, ; Linhartova et al, ), so DNA fragmentation is a reliable metric of free‐radical mediated damage induced by oxidative stress (Kodoma et al, ). The results presented here indicate the accumulation of oxidative damage in a time‐ and dose‐dependent manner in sperm samples incubated with X‐XO.…”

Section: Discussionmentioning

confidence: 99%

The effect of reactive oxygen species on motility parameters, DNA integrity, tyrosine phosphorylation and phosphatase activity of common carp (Cyprinus carpioL.) spermatozoa

Gazo

Shaliutina‐Kolešová

Dietrich

et al. 2014

Molecular Reproduction Devel

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The effect of reactive oxygen species production on the motility parameters, DNA integrity, acid phosphatase activity, and protein tyrosine phosphorylation in spermatozoa of the common carp (Cyprinus carpio) was investigated. Spermatozoa were exposed to different concentrations of xanthine and xanthine oxidase (X-XO) either in the presence or absence of antioxidants for 15 and 60 min. A dose- and time-dependent reduction in spermatozoa motility and velocity was observed. Comet assays showed a dramatic increase in DNA fragmentation after 15 min. Changes in tyrosine phosphorylation of spermatozoa proteins were observed by Western blotting with anti-phosphotyrosine antibodies, and proteins of interest were identified by mass spectrometry. After a 60 min exposure to X-XO, O-linked N-acetylglucosamine transferase, isoform 4 was phosphorylated and septin-8-A was dephosphorylated. Acid phosphatase activity also decreased in a dose-dependent manner after a 60 min exposure to oxidative stress. The results demonstrate that oxidative stress impaired functional variables (sperm motility, velocity, DNA integrity) of carp spermatozoa, and altered intracellular signalling pathways through changes in tyrosine phosphorylation and acid phosphatase activity.

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“…Studies have been conducted about impact of toxicants on sperm motility of different fish species (Salmo trutta fario, Cyprinus carpio, Acipenser ruthenus, Heteropneustes fossilis, Rutilus frisii kutum, O. mykiss). 21,26,36,[40][41][42][43][44][45][46][47][48] In these studies about fish sperm, it has been reported lower spermatozoon motility with exposure to different pesticides. Singh and Singh 26 stated that sperm motility and duration of Heteropneustes fossilis were influenced by CYP and 1 ppm was lethal on sperm motility.…”

Section: Discussionmentioning

confidence: 99%

Cypermethrin-Induced in vitro Alterations on Oxidative Stress and Quality of Salmo coruhensis Spermatozoa

Kutluyer¹,

Kocabaş²,

Erişir³

et al. 2018

IJPER

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The use of insecticides has been increasing along with increasing agriculture activities and has caused deleterious environmental impacts. Non-target organisms in particular, including fish, are affected by pesticides. In this work, the impacts of cypermethrin (CYP) on sperm oxidative stress markers and sperm motility were investigated in vitro. The CYP concentrations were 0 μg L -1 control, ethanol), 1.025 μg L (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT)] activities in sperm cells were examined for determination of oxidative stress status. Our findings showed that motility and survival of sperm cells significantly decreased with exposure to CYP. Biochemical assays revealed that CAT activity and levels of MDA and, GSH increased in spermatozoa based on CYP concentration while activity of GSH-Px and SOD decreased. Consequently, spermatozoa were highly sensitive to CYP exposure. It can be deduced that CYP has the potential to disrupt sperm quality and to cause to oxidative stress in sperm cells of S. coruhensis.

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The in vitro effect of duroquinone on functional competence, genomic integrity, and oxidative stress indices of sterlet (Acipenser ruthenus) spermatozoa

Cited by 27 publications

References 38 publications

Chemical and Toxicological Characterization of Vaping Emission Products from Commonly Used Vape Juice Diluents

Chemical and Toxicological Characterization of Vaping Emission Products from Commonly Used Vape Juice Diluents

The effect of reactive oxygen species on motility parameters, DNA integrity, tyrosine phosphorylation and phosphatase activity of common carp (Cyprinus carpioL.) spermatozoa

Cypermethrin-Induced in vitro Alterations on Oxidative Stress and Quality of Salmo coruhensis Spermatozoa

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