A niche in a dish: pericytes support HSC

Levesque, Jean‐Pierre

doi:10.1182/blood-2013-02-485144

Cited by 13 publications

(11 citation statements)

References 10 publications

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“…Therefore, Nestin is known as a neural marker and its presence can be considered as a criterion for the ability to differentiate into neurons [18, 22]. However, Nestin has shown to be expressed by other cell types such as hair follicle stem cells [23], pericytes [24], endothelial cells [25], myofibroblasts, and pancreatic fibroblasts [26]. Therefore, analysis on expression of other specific neuron markers such as Tub3 [27, 28] and MAP2 [29, 30] has been done concurrently for neuronal confirmation.…”

Section: Introductionmentioning

confidence: 99%

Differentiation of Dental Pulp Stem Cells into Neuron-Like Cells in Serum-Free Medium

Ariffin

Kermani

Abidin

et al. 2013

Stem Cells International

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Dental pulp tissue contains dental pulp stem cells (DPSCs). Dental pulp cells (also known as dental pulp-derived mesenchymal stem cells) are capable of differentiating into multilineage cells including neuron-like cells. The aim of this study was to examine the capability of DPSCs to differentiate into neuron-like cells without using any reagents or growth factors. DPSCs were isolated from teeth extracted from 6- to 8-week-old mice and maintained in complete medium. The cells from the fourth passage were induced to differentiate by culturing in medium without serum or growth factors. RT-PCR molecular analysis showed characteristics of Cd146+, Cd166+, and Cd31− in DPSCs, indicating that these cells are mesenchymal stem cells rather than hematopoietic stem cells. After 5 days of neuronal differentiation, the cells showed neuron-like morphological changes and expressed MAP2 protein. The activation of Nestin was observed at low level prior to differentiation and increased after 5 days of culture in differentiation medium, whereas Tub3 was activated only after 5 days of neuronal differentiation. The proliferation of the differentiated cells decreased in comparison to that of the control cells. Dental pulp stem cells are induced to differentiate into neuron-like cells when cultured in serum- and growth factor-free medium.

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Section: Introductionmentioning

confidence: 99%

Differentiation of Dental Pulp Stem Cells into Neuron-Like Cells in Serum-Free Medium

Ariffin

Kermani

Abidin

et al. 2013

Stem Cells International

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“…71 Impaired Notch signaling via both anti-Notch1 antibodies and by inhibition of gamma-secretase decreased the CD146-mediated hematopoietic stem cell support by CD146+ cells. 72 These data collectively suggest that a subset of human stromal cells which are CD146+ is most likely responsible for support of human MDS engraftment in xenograft models.…”

Section: Mds Cells Require Their Nichementioning

confidence: 80%

Biology of BM failure syndromes: role of microenvironment and niches

Balderman

Calvi

2014

Hematology

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The marrow microenvironment and its components regulate hematopoietic stem and progenitor cell (HSC) fate. An abnormality in the marrow microenvironment and specific dysfunction of the HSC niche could play a critical role in initiation, disease progression and response to therapy of marrow failure syndromes. Therefore, the identification of changes in the HSC niche in marrow failure syndromes should lead to further knowledge of the signals that disrupt the normal microenvironment. In turn, niche disruption may contribute to disease morbidity resulting in pancytopenia and clonal evolution, and its understanding could point to new therapeutic targets for these conditions. We briefly (a) review evidence for the importance of the marrow microenvironment as a regulator of normal hematopoiesis, (b) summarize the current knowledge regarding the role of dysfunctions in the marrow microenvironment in marrow failure syndromes, and (c) propose a strategy through which niche stimulation can complement current treatment for MDS.

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“…As distinct from Corselli et al, who mentioned that the supportive effect of CD146 + pericytes required cell-to-cell contact (Notch ligand Jagged-1, abundantly expressed at the surface of these pericytes, interacting with its receptor Notch1 expressed on HSCs (Corselli et al, 2013)), we found the greatest HPC expansion with pericyte cocultures (2.8-fold vs. the control) without contact and we obtained a high frequency of CD34 +, CD38 +, CD34 + CD41 +, and CD235 + cells with pericyte cocultures and pericyte CM. In this work, we tried to answer the question addressed by Levesque of whether CD146 + pericytes could support the actual ex vivo expansion of human reconstituting HSCs by quantifying the content in reconstituting HSCs before and after coculture (Levesque, 2013).…”

Section: Discussionmentioning

confidence: 99%

Expansion of human umbilical cord blood hematopoieticprogenitors with cord vein pericytes

Saltik¹,

Yağcı²

2017

Turk J Biol

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The vascular niche is a site rich in blood vessels, whereas endothelial cells, pericytes, and smooth muscle cells create a microenvironment that recruits mesenchymal stem cells (MSCs) and hematopoietic stem cells (HSCs), which is important for stem cell mobilization, proliferation, and differentiation. In this study, CD146 + pericytes were purified and enriched from the human umbilical cord vein. In order to define their direct role in hematopoiesis, we tested the CD146 + pericytes as compared with osteoblasts derived from umbilical cord blood (UCB) MSCs to sustain human UCB hematopoietic progenitor cells in noncontact coculture settings or in culture media previously conditioned (CM) by these cells. The growth of UCB cells was the greatest in pericyte cocultures (2.8-fold vs. the control). The increased growth in pericyte and pericyte CM cultures was largely the result of increased frequency of CD34 + and CD38 + hematopoietic progenitors, CD34 + CD41 + megakaryocyte progenitors, and CD235 + erythroblasts. A total of 29 factors were found to be secreted by pericytes higher than by osteoblasts. The most secreted growth factor by pericytes was vascular endothelial growth factor (1.3-fold). We demonstrate for the first time that human CD146 + perivascular cell coculture and CM are able to directly support the ex vivo maintenance of human hematopoietic progenitor cells.

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A niche in a dish: pericytes support HSC

Cited by 13 publications

References 10 publications

Differentiation of Dental Pulp Stem Cells into Neuron-Like Cells in Serum-Free Medium

Differentiation of Dental Pulp Stem Cells into Neuron-Like Cells in Serum-Free Medium

Biology of BM failure syndromes: role of microenvironment and niches

Expansion of human umbilical cord blood hematopoieticprogenitors with cord vein pericytes

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