Genetic diversity, fixation and differentiation of the freshwater snail Biomphalaria pfeifferi (Gastropoda, Planorbidae) in arid lands

Nguema, Rodrigue Mintsa; Langand, Juliette; Galinier, Richard; Idris, Mohamed A.; Shaban, Mahmoud A.; Yafae, Salem Al; Moné, Hélène; Mouahid, Gabriel

doi:10.1007/s10709-013-9715-8

Cited by 10 publications

(2 citation statements)

References 38 publications

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“…pfeifferi because it differs from other major S . mansoni -transmitting snail species in that it is a strong preferential selfing species, a characteristic potentially resulting in low genetic diversity within populations [ 20 – 23 ]. Our relative ignorance regarding B .…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic responses of Biomphalaria pfeifferi to Schistosoma mansoni: Investigation of a neglected African snail that supports more S. mansoni transmission than any other snail species

Buddenborg

Zhang

et al. 2017

PLoS Negl Trop Dis

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BackgroundBiomphalaria pfeifferi is highly compatible with the widespread human-infecting blood fluke Schistosoma mansoni and transmits more cases of this parasite to people than any other snail species. For these reasons, B. pfeifferi is the world’s most important vector snail for S. mansoni, yet we know relatively little at the molecular level regarding the interactions between B. pfeifferi and S. mansoni from early-stage sporocyst transformation to the development of cercariae.Methodology/Principal findingsWe sought to capture a portrait of the response of B. pfeifferi to S. mansoni as it occurs in nature by undertaking Illumina dual RNA-Seq on uninfected control B. pfeifferi and three intramolluscan developmental stages (1- and 3-days post infection and patent, cercariae-producing infections) using field-derived west Kenyan specimens. A high-quality, well-annotated de novo B. pfeifferi transcriptome was assembled from over a half billion non-S. mansoni paired-end reads. Reads associated with potential symbionts were noted. Some infected snails yielded fewer normalized S. mansoni reads and showed different patterns of transcriptional response than others, an indication that the ability of field-derived snails to support and respond to infection is variable. Alterations in transcripts associated with reproduction were noted, including for the oviposition-related hormone ovipostatin and enzymes involved in metabolism of bioactive amines like dopamine or serotonin. Shedding snails exhibited responses consistent with the need for tissue repair. Both generalized stress and immune factors immune factors (VIgLs, PGRPs, BGBPs, complement C1q-like, chitinases) exhibited complex transcriptional responses in this compatible host-parasite system.SignificanceThis study provides for the first time a large sequence data set to help in interpreting the important vector role of the neglected snail B. pfeifferi in transmission of S. mansoni, including with an emphasis on more natural, field-derived specimens. We have identified B. pfeifferi targets particularly responsive during infection that enable further dissection of the functional role of these candidate molecules.

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Section: Introductionmentioning

confidence: 99%

Transcriptomic responses of Biomphalaria pfeifferi to Schistosoma mansoni: Investigation of a neglected African snail that supports more S. mansoni transmission than any other snail species

Buddenborg

Zhang

et al. 2017

PLoS Negl Trop Dis

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“…For each chronotype, the study was conducted twice, using two populations of B. pfeifferi originating from two waterbodies, Siginitti 54°28′08.99″ (E); 17°08′36.44″ (N) (Experiment 1) and Tibraq 54°19′35.09″ (E); 17°05′58.11″ (N) (Experiment 2). We considered these experiments as equivalent since the snails were originating from the same drainage basin (Wustah mountains, Dhofar, Oman) and since they were shown to segregate in PCA analysis based on microsatellites genetic comparison . The snail mean diameters at the beginning of the experiments are presented in Table ; they were not significantly different whatever the chronotype and whatever the experiment.…”

Section: Methodsmentioning

confidence: 99%

Host‐parasite life‐histories of the diurnalvs. nocturnal chronotypes ofSchistosoma mansoni: adaptive significance

Mouahid

Nguema

Mashikhi

et al. 2019

Tropical Med Int Health

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objectives To optimise host-to-host transmission, digenean trematodes (parasites) synchronise their cercarial emission patterns with the aquatic activities of their vertebrate hosts. Schistosoma mansoni has two different circadian chronotypes: a diurnal shedding pattern with a mean peak occurring at 11:00 h, and a nocturnal shedding pattern with a mean peak occurring at 20:00 h. We analysed the life-history variations between these two chronotypes at the levels of the parasite and its hosts.methods For each chronotype, we quantified three life-history traits related to the parasite (prepatent period, infection rate and cercarial production) and analysed the morphometry and the morphology of the parasite eggs; we also quantified three life-history traits related to the snail intermediate host (shell diameter, fecundity and survival rate) and one life-history trait related to the experimental definitive host (survival rate). A phylogeny based on the mitochondrial cytochromeoxidase gene was made on samples of both chronotypes.results Life-history analysis revealed significant variations between the two chronotypes. Lifehistory traits were optimal for both the parasite and the snail host for the diurnal chronotype compared to the nocturnal one. The new chronotype behaved like an allopatric population towards its snail host. Phylogenetic analysis supports the hypothesis of a lateral transfer of S. mansoni from humans to Rattus rattus. These results were interpreted in terms of an ongoing sympatric speciation.conclusion The nocturnal chronotype of S. mansoni showed non-adapted life-history traits in its relation with the snail intermediate host Biomphalaria pfeifferi. The emergence of this new phenotype is probably linked to divergent natural selection.keywords Schistosoma mansoni, nocturnal chronotype, cercarial emergence pattern, circadian rhythm, sympatric speciation

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Analysis of genetic diversity and population structure of Bellamya quadrata from lakes of middle and lower Yangtze River

Zhang

Zhou

et al. 2015

Genetica

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As an endemic species of freshwater gastropods in China, Bellamya quadrata plays an important role in ecosystem service provision and commercial importance. However, the species is overharvested and its natural habitats are under severe threat due to fragmentation and loss. To estimate the genetic diversity and population structure of B. quadrata, 285 individuals from eight lake populations across middle and lower Yangtze River were sampled. Seven microsatellite loci were genotyped. Our results showed that (i) the genetic diversity of B. quadrata was high in most of the studied populations, yet effective population sizes appear to be rather small in some populations; (ii) low levels of genetic differentiation exists among populations but gene flow was generally high; (iii) no clear geographic or genetic structure was observed in the studied region, implying mechanisms (zoochoric dispersal and anthropogenic translocations) that enhance dispersal and gene flow have promoted population connectivity. However, the comparatively high genetic diversity of B. quadrata could be attributed to a lag phase, suggesting that the genetic diversity of this species may be lost in the future and the priorities for conservation of B. quadrata are necessary.

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Genetic diversity, fixation and differentiation of the freshwater snail Biomphalaria pfeifferi (Gastropoda, Planorbidae) in arid lands

Cited by 10 publications

References 38 publications

Transcriptomic responses of Biomphalaria pfeifferi to Schistosoma mansoni: Investigation of a neglected African snail that supports more S. mansoni transmission than any other snail species

Transcriptomic responses of Biomphalaria pfeifferi to Schistosoma mansoni: Investigation of a neglected African snail that supports more S. mansoni transmission than any other snail species

Host‐parasite life‐histories of the diurnalvs. nocturnal chronotypes ofSchistosoma mansoni: adaptive significance

Analysis of genetic diversity and population structure of Bellamya quadrata from lakes of middle and lower Yangtze River

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