N-3 Polyunsaturated Fatty Acids Supplementation Does not Affect Changes of Lipid Metabolism Induced in Rats by Altered Thyroid Status

Abstract

Epidemiological studies have demonstrated that n-3 polyunsaturated fatty acid (PUFA) consumption is associated with a reduced risk of atherosclerosis and hyperlipidemia. It is well known that lipid metabolism is also influenced by thyroid hormones. The aim of our study was to test whether n-3 PUFA supplementation (200 mg/kg of body weight/day for 6 weeks given intragastrically) would affect lipid metabolism in Lewis male rats with altered thyroid status. Euthyroid, hypothyroid, and hyperthyroid status of experimental groups was well defined by plasma levels of triiodothyronine, the activity of liver mitochondrial glycerol-3-phosphate dehydrogenase, and by relative heart weight. Fasting blood glucose levels were significantly higher in the hyperthyroid compared to the euthyroid and hypothyroid rats (5.0±0.2 vs. 3.7±0.4 and 4.4±0.2 mmol/l, respectively). In hyperthyroid animals, the concentration of plasma postprandial triglycerides was also increased compared to euthyroid and hypothyroid rats (0.9±0.1 vs. 0.5±0.1 and 0.4±0.1 mmol/l, respectively). On the other hand, hypothyroidism compared to euthyroid and hyperthyroid status was associated with elevated plasma levels of total cholesterol (2.6±0.2 vs. 1.5±0.1 and 1.6±0.1 mmol/l, respectively), LDL cholesterol (0.9±0.1 vs. 0.4±0.1 and 0.2±0.1 mmol/l, respectively) as well as HDL cholesterol (1.6±0.1 vs. 1.0±0.1 and 1.3±0.1 mmol/l, respectively). Supplementation of n-3 PUFA in the present study did not significantly modify either relative heart weight or glucose and lipid levels in any thyroid status.

Red Palm Oil Supplementation Does Not Increase Blood Glucose or Serum Lipids Levels in Wistar Rats With Different Thyroid Status

“…In the present study HT rats manifested signs of hyperglycemia regardless of RPO supplementation. We obtained very similar values of fasting glucose levels in our previous experiments on Lewis rats with altered thyroid status (Rauchová et al 2013).…”

“…In the study, we changed our preceding protocol for inducing HT status in the rats, using oral administration of T 3 and T 4 combination in drinking water, similarly as was done by Szeiffová et al (2017). This protocol seems to be more appropriate for the animals as compared to previously applied intraperitoneal administration of T 3 only (Rauchová et al 2004, Rauchová et al 2013. The serum levels of total and free T 3 and T 4 in the present study reflected well the achievement of the chronic HY and HT status in the treated rats.…”

“…We observed no significant differences in body weight of the rats in three different thyroid groups with or without RPO supplementation. Similarly, our previous studies did not reveal any significant differences in body weights among three experimental groups (EU, HY, HT) of Lewis and Wistar rats with thyroid hormones treatment lasting few weeks, respectively (Rauchová et al 2013, Szeiffová et al 2017. Nevertheless, the absolute and relative heart weights clearly demonstrated the influence of thyroid status.…”

Slow to fast muscle transformation following heterochronous isotransplantation is influenced by host thyroid hormone status

“…The hearts and thyroid glands were excised and weighed. Dissected livers were processed as described earlier to measure GPDH activity (Rauchova et al 2004(Rauchova et al , 2011(Rauchova et al , 2013.…”

Parathyroid Hormone Replacement Therapy in Hypoparathyroidism: A Meta-Analysis

“…PTH-replacement therapy may also lower urinary calcium, improve bone quality, reduce ectopic tissue calcification, and improved quality of life [23,24]. In addition, there is evidence that hypoparathyroidism is associated with elevated cholesterol levels, a risk factor for cardiovascular disease [25]. It is unclear if PTH-replacement therapy would affect cholesterol levels.…”