Esculentin(1-21), an amphibian skin membrane-active peptide with potent activity on both planktonic and biofilm cells of the bacterial pathogen Pseudomonas aeruginosa

Luca, Vincenzo; Stringaro, Annarita; Colone, Marisa; Pini, Alessandro; Mangoni, Maria Luisa

doi:10.1007/s00018-013-1291-7

Cited by 147 publications

(133 citation statements)

References 67 publications

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“…Survival of bacterial cells at different time intervals was expressed as percentage with respect to the initial number (time zero). 14,28 All data are the mean of three independent experiments ± SD.…”

Section: ■ Methodsmentioning

confidence: 99%

“…12 A related question is whether peptide concentrations in vivo are high enough to perturb the permeability of bacterial membranes. 13 In vitro microbiological studies determine the lowest AMP concentration that inhibits bacterial growth (minimum inhibitory concentration, or MIC) or that causes a reduction in the number of viable bacteria ≥99.9% (minimum bactericidal concentration, or MBC 14 ). However, these values strongly depend on the conditions of the assay.…”

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confidence: 99%

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How Many Antimicrobial Peptide Molecules Kill a Bacterium? The Case of PMAP-23

Luca²,

et al. 2014

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Antimicrobial peptides (AMPs) kill bacteria mainly through the perturbation of their membranes and are promising compounds to fight drug resistance. Models of the mechanism of AMPs-induced membrane perturbation were developed based on experiments in liposomes, but their relevance for bacterial killing is debated. We determined the association of an analogue of the AMP PMAP-23 to Escherichia coli cells, under the same experimental conditions used to measure bactericidal activity. Killing took place only when bound peptides completely saturated bacterial membranes (10 6 −10 7 bound peptides per cell), indicating that the "carpet" model for the perturbation of artificial bilayers is representative of what happens in real bacteria. This finding supports the view that, at least for this peptide, a microbicidal mechanism is possible in vivo only at micromolar total peptide concentrations. We also showed that, notwithstanding their simplicity, liposomes represent a reliable model to characterize AMPs partition in bacterial membranes.

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Section: ■ Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

How Many Antimicrobial Peptide Molecules Kill a Bacterium? The Case of PMAP-23

Luca²,

et al. 2014

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“…Biofilm formation was performed as previously described in (Ceri et al 2001;Falciani et al 2012;Feng et al 2013;Luca et al 2013;Almaaytah et al 2014) employing the Calgary biofilm device (Innovotech, Canada). Briefly, The Gram positive S. aureus (29213) and S. aureus (33591) in addition to the Gram negative P. aeruginosa (27853) and P. aeruginosa (BAA2114) bacterial strains were incubated in Mueller-Hinton broth (MHB) for 20 h at 37°C, and cultures were diluted in the same medium to achieve a concentration of 10 7 CFU/ml.…”

Section: Biofilm Formationmentioning

confidence: 99%

In Vitro Synergistic Activities of the Hybrid Antimicrobial Peptide MelitAP-27 in Combination with Conventional Antibiotics Against Planktonic and Biofilm Forming Bacteria

Almaaytah

Alnaamneh

Abualhaijaa

et al. 2016

Int J Pept Res Ther

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The extensive use of antibiotics for the treatment of human infections during the last few decades has led to a dramatic increase in the emergence of multidrug-resistant bacteria (MDRB) among various bacterial strains. Global research is currently focused on finding novel alternative agents with different mechanisms of action rather than the use of conventional antibiotics to counteract the threat of bacterial and biofilm infections. Antimicrobial peptides represent promising alternative agents for conventional antibiotics as these molecules display a broad spectrum of activity against several microorganisms. Recently, we have designed a novel hybrid antimicrobial peptide named MelitAP-27. This peptide has been found to display potent broad spectrum and selective in vitro antimicrobial activities against a wide range of Gram-positive and Gramnegative bacteria. In the present study, the in vitro antimicrobial and antibiofilm activities of the peptide alone and in combination with five different types of antibiotics were assessed against wild-type and resistant Gram-positive and Gram-negative bacterial strains. Our results showed that most of the combination groups displayed a synergistic mode of action against planktonic and biofilm forming bacteria which resulted in decreasing the effective MIC values for MelitAP-27 to the nanomolar concentrations. These effective concentrations were associated with negligible toxicities on mammalian cells. The results of our study indicate that combinations of MelitAP-27 with conventional antibiotics may be pursued as a potential novel treatment strategy against MDRB and biofilm forming bacteria.

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“…Moreover, they are not toxic toward mammalian cells e.g. erythrocytes, lymphocytes, macrophages and epithelial cells at their microbicidal concentration [54,63,64,66,79].…”

Section: In Vitro Antibacterial Activities Of Esc-1a(1-21)nh 2 and Esmentioning

confidence: 99%

“…culture on special plastic plates with lids with 96 embedded pegs [64]. Analogous to what was found for the planktonic form, Esc-1a(1-21)NH 2 exhibited a stronger anti-biofilm activity than Esc-1b(1-18)NH 2 , inducing 3-log reduction in the number of viable biofilm cells within 2 h at 12 M compared to 24 M, in the case of Esc-1b(1-18)NH 2 (Table 4).…”

Section: Peptidementioning

confidence: 99%