Structural elucidation of an extracellular polysaccharide produced by the marine fungus Aspergillus versicolor

Chen, Yin; Mao, Wenjun; Gao, Yan; Teng, Xiancun; Zhu, Weiming; Chen, Yanli; Zhao, Chunqi; Li, Na; Wang, Chunyan; Yan, Ming; Shan, Jimiao; Cong, Lin; Guo, Tao

doi:10.1016/j.carbpol.2012.12.047

Cited by 43 publications

(18 citation statements)

References 39 publications

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“…After dialysis against water, crude EPSs are generally stored as vacuum-dried or lyophilized powder. The next step of EPS purification is deproteinization thereof using Sevage reagent (Chen et al 2011 , 2013a , b , c ). The main methods used for purification of Ascomycota and Basidiomycota EPSs are Ion Exchange Chromatography (IEC) and a type of Size Exclusion Chromatography (SEC)–Gel Permeation Chromatography (GPC) (Table 2 ) (Shu and Lung 2004 ; Chen et al 2011 ; Ma et al 2015 ).…”

Section: Production Of Eps By Ascomycota and Basidiomycota Fungal Strmentioning

confidence: 99%

“…Y16 95 % ethanol (1/3) 48 Water IEC GPC Water EPS1-Man, Gal EPS2-Man, Glc EPS1—1 → 2;1 → 6, EPS2—1 → 3; 1 → 6 EPS1-15.0 EPS2-6.0 Chen et al ( 2011 ) Aspergillus sp. RYLF17 95 % ethanol (1/4), 24 h, 4 °C – – – – – – – Yadava et al ( 2012 ) Aspergillus versicolor 95 % ethanol (1/3) 48 Water IEC GPC Water Glc, Man α-1 → 6 500.0 Chen et al ( 2013a ) Aureobasidium pullulans RYLF-10 95 % ethanol (1/4), 24 h, 4 °C – Water – – – – – Yadav et al ( 2014 ) Botryosphaeria rhodina MMLR Absolute ethanol (1/3) 48 GPC Water, 1 M NaOH Glc β-1 → 6 – Barbosa et al ( 2003 ) and Vasconcelos et al ( 2008 ) Botryosphaeria rhodina MMGR Absolute ethanol (1/3) 48 …”

Section: Production Of Eps By Ascomycota and Basidiomycota Fungal Strmentioning

confidence: 99%

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Extracellular polysaccharides from Ascomycota and Basidiomycota: production conditions, biochemical characteristics, and biological properties

Osińska-Jaroszuk

Jarosz-Wilkołazka

Jaroszuk-Ściseł

et al. 2015

World J Microbiol Biotechnol

107

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Fungal polysaccharides (PSs) are the subject of research in many fields of science and industry. Many properties of PSs have already been confirmed and the list of postulated functions continues to grow. Fungal PSs are classified into different groups according to systematic affinity, structure (linear and branched), sugar composition (homo- and heteropolysaccharides), type of bonds between the monomers (β-(1 → 3), β-(1 → 6), and α-(1 → 3)) and their location in the cell (cell wall PSs, exoPSs, and endoPSs). Exopolysaccharides (EPSs) are most frequently studied fungal PSs but their definition, classification, and origin are still not clear and should be explained. Ascomycota and Basidiomycota fungi producing EPS have different ecological positions (saprotrophic and endophytic, pathogenic or symbiotic-mycorrhizae fungi); therefore, EPSs play different biological functions, for example in the protection against environmental stress factors and in interactions with other organisms. EPSs obtained from Ascomycota and Basidiomycota fungal cultures are known for their antioxidant, immunostimulating, antitumor, and antimicrobial properties. The major objective of the presented review article was to provide a detailed description of the state-of-the-art knowledge of the effectiveness of EPS production by filamentous and yeast Ascomycota and Basidiomycota fungi and techniques of derivation of EPSs, their biochemical characteristics, and biological properties allowing comprehensive analysis as well as indication of similarities and differences between these fungal groups. Understanding the role of EPSs in a variety of processes and their application in food or pharmaceutical industries requires improvement of the techniques of their derivation, purification, and characterization. The detailed analyses of data concerning the derivation and application of Ascomycota and Basidiomycota EPSs can facilitate development and trace the direction of application of these EPSs in different branches of industry, agriculture, and medicine.

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Section: Production Of Eps By Ascomycota and Basidiomycota Fungal Strmentioning

confidence: 99%

Section: Production Of Eps By Ascomycota and Basidiomycota Fungal Strmentioning

confidence: 99%

Extracellular polysaccharides from Ascomycota and Basidiomycota: production conditions, biochemical characteristics, and biological properties

Osińska-Jaroszuk

Jarosz-Wilkołazka

Jaroszuk-Ściseł

et al. 2015

World J Microbiol Biotechnol

107

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“…Signals between 950 cm −1 and 1200 cm −1 indicated the pyranoid rings presenting in LEP-1 [28]. The band at 916 cm −1 was due to the asymmetric telescopic vibration of glucopyranose rings [11]. The weak absorption at 883 cm −1 demonstrated the presence of ␤-anomeric configurations [33], while the characteristic absorption at 816 cm −1 indicated the existence of ␣-configuration(s) in the polysaccharide.…”

Section: Structural Characterization Of Lep-1mentioning

confidence: 95%

“…The LEP-1 was extracted and purified according to the methods of He et al and Chen et al with minor modifications [6,11]. The seed of Lachnum YM268 was inoculated into a 100 L fermentation tank, and then fermented at 26 • C for 8 d (160 r min −1 ).…”

Section: Extraction and Purificationmentioning

confidence: 99%

Structural characterization and anti-hypoxia activity of an exopolysaccharide isolated from fermentation broth of Lachnum sp.

Zong

Surhio

et al. 2016

Process Biochemistry

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“…This represents the fingerprint region for carbohydrates and is dominated by ring vibrations overlapped with stretching vibrations of C-OH side groups and C-O-C glycosidic band vibration [30] RBP displayed absorption at 1145 cm -1 , 1058 cm -1 , and 1027 cm -1 , which was due to pyranose. In the anomeric region (950-700cm -1 ), the polysaccharide exhibited obvious characteristic adsorption at 812 cm -1 , indicating the glucopyranose units in RBPs [31]. The β-conformer had absorption peaks near 874 cm −1 , and the α-conformer region was near 812 cm −1 [32,8].…”

Section: Ftir Spectroscopymentioning

confidence: 99%

Effect of Various Temperatures on Bletillae Rhizoma Polysaccharide Extraction and Physicochemical Properties

et al. 2018

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Six fractional polysaccharides were prepared by water extraction and alcohol precipitation under controlled temperature from bletillae rhizoma, a traditional Chinese medicine. Based on this, yields of bletillae rhizome polysaccharides (RBPs) were obtained. The extracting temperature impacted the characteristics of the fractional polysaccharides. The fractional polysaccharides were characterized by glucomannan (GM) content, thermal stability, scanning electron microscopy (SEM), Fourier-transform infrared (FTIR) spectroscopy, gel permeation chromatography (GPC), and x-ray diffraction (XRD). For the analysis, 2.0% w/v dispersions of the six fractional polysaccharides were prepared and their flow behaviors were evaluated using a rotational rheometer. The results showed that increased extraction temperature led to increased GM extraction yields and extraction rate, but GM content was relative stable (over 90%). The average molecular weight (Mw) of fractional polysaccharides obtained at 30, 40, 50, 60, 70, and 80 °C was 3.598 × 104, 4.188 × 104, 8.632 × 104, 8.850 × 104, 2.372 × 105, and 3.081 × 105 g/mol, respectively. SEM revealed that fractional polysaccharides had a porous structure of different sizes and densities. Thermal analysis, FTIR, and XRD results indicated that extraction temperature affects the structure and moisture content of fractional polysaccharides. All results showed that the extraction temperature has an obvious impact on the morphology, molecular weight, and polydispersity of the RBPs. This simple process is a promising method for the preparation of fractional polysaccharides.

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Structural elucidation of an extracellular polysaccharide produced by the marine fungus Aspergillus versicolor

Cited by 43 publications

References 39 publications

Extracellular polysaccharides from Ascomycota and Basidiomycota: production conditions, biochemical characteristics, and biological properties

Extracellular polysaccharides from Ascomycota and Basidiomycota: production conditions, biochemical characteristics, and biological properties

Structural characterization and anti-hypoxia activity of an exopolysaccharide isolated from fermentation broth of Lachnum sp.

Effect of Various Temperatures on Bletillae Rhizoma Polysaccharide Extraction and Physicochemical Properties

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