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Abstract

Objectives: Type 1 diabetes mellitus is an emerging epidemic worldwide and results from autoimmune destruction of insulin-producing β cells. Islet transplanting is a potential treatment for type 1 diabetes mellitus. Materials and Methods: The Shiraz Organ Transplant Center is a leading center for organ transplants, especially pancreatic transplants, in Iran. For this reason, we want to establish an islet transplanting program. Here, we briefly describe our experience with islet isolation on 6 pancreata from deceased donors. We discussed the necessary equipment required for this procedure, as well as the professionals needed and a specially planned facility. Results: Islet yield was ≤ 100 000 (islet equivalent), viability 40% to 45%, and the purity was 30% to 45%. We do not have a refrigerated COBE processor for purification; therefore, the yield was low. Our experience shows that we should improve things, so as to acquire more islets for developing clinical grade cell therapy. Conclusions: Overall, isolation costs are high, and accessing a safer, more economic, and persistent source of material and reagents will improve this technique.

Protective effect of nobiletin on isolated human islets survival and function against hypoxia and oxidative stress-induced apoptosis

“…After mechanical and enzymatic digestion in a Ricordi chamber, the islets purification was performed in a continuous Biocoll (Biochrom, Germany) gradient in a cell sorter processor called COBE 2991. The counting and purity of the islet were assessed by dithizone staining and the number of islets was expressed in the islet equivalents (IEQ) 44 . The isolated islets with more than 80% purity were used to study.…”