High-Fat Diet-Mediated Lipotoxicity and Insulin Resistance Is Related to Impaired Lipase Expression in Mouse Skeletal Muscle

Badin, Pierre Marie; Vila, Isabelle K.; Louche, Katie; Mairal, Aline; Marqués, Marie-Adeline; Bourlier, Virginie; Tavernier, Geneviève; Langin, Dominique; Moro, Cédric

doi:10.1210/en.2012-2029

Cited by 70 publications

(53 citation statements)

References 63 publications

(74 reference statements)

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“…Six hour-fasted mice were injected intraperitoneally with a bolus of D-glucose at 2 g/kg (Sigma-Aldrich, Saint-Quentin Fallavier, France) and insulin 0.5 units/kg (Insuman Rapid; Sanofi, Paris, France) for glucose and insulin tolerance tests (GTT and ITT), respectively (29). Blood glucose levels were monitored from the tip of the tail with a glucometer (Accucheck; Roche, Meylan, France) at 0, 15, 30, 45, 60, and 90 min after injection.…”

Section: Glucose and Insulin Tolerance Testsmentioning

confidence: 99%

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Defective Natriuretic Peptide Receptor Signaling in Skeletal Muscle Links Obesity to Type 2 Diabetes

et al. 2015

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Circulating natriuretic peptide (NP) levels are reduced in obesity and predict the risk of type 2 diabetes (T2D). Since skeletal muscle was recently shown as a key target tissue of NP, we aimed to investigate muscle NP receptor (NPR) expression in the context of obesity and T2D. Muscle NPRA correlated positively with wholebody insulin sensitivity in humans and was strikingly downregulated in obese subjects and recovered in response to diet-induced weight loss. In addition, muscle NP clearance receptor (NPRC) increased in individuals with impaired glucose tolerance and T2D. Similar results were found in obese diabetic mice. Although no acute effect of brain NP (BNP) on insulin sensitivity was observed in lean mice, chronic BNP infusion improved blood glucose control and insulin sensitivity in skeletal muscle of obese and diabetic mice. This occurred in parallel with a reduced lipotoxic pressure in skeletal muscle due to an upregulation of lipid oxidative capacity. In addition, chronic NP treatment in human primary myotubes increased lipid oxidation in a PGC1a-dependent manner and reduced palmitate-induced lipotoxicity. Collectively, our data show that activation of NPRA signaling in skeletal muscle is important for the maintenance of long-term insulin sensitivity and has the potential to treat obesity-related metabolic disorders.

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Section: Glucose and Insulin Tolerance Testsmentioning

confidence: 99%

“…Blood glucose levels were monitored from the tip of the tail with a glucometer (Accucheck; Roche, Meylan, France) at 0, 15, 30, 45, 60, and 90 min after injection. Radiolabeled GTTs were performed as previously described (29).…”

Section: Glucose and Insulin Tolerance Testsmentioning

confidence: 99%

Defective Natriuretic Peptide Receptor Signaling in Skeletal Muscle Links Obesity to Type 2 Diabetes

et al. 2015

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“…According to Badin et al ( 66 ), in skeletal muscle of HFD-fed mice, CGI58 is induced, while ATGL expression is repressed. These authors report signifi cant changes in skeletal muscle DAG and membrane translocation of protein kinase C subtypes (PKC-and PKC-).…”

Section: Unanswered Questionsmentioning

confidence: 99%

Fatty acid elongase-5 (Elovl5) regulates hepatic triglyceride catabolism in obese C57BL/6J mice

Tripathy

Lytle

Stevens

et al. 2014

Journal of Lipid Research

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This article is available online at http://www.jlr.org populations. NAFLD is the hepatic manifestation of metabolic syndrome and is associated with a cluster of metabolic risk factors including obesity, dyslipidemia, type 2 diabetes, and hypertension ( 1 ). The prevalence of NAFLD is ‫ف‬ 30% in US adults but is as high as 90-95% in obese individuals and у 60% in type 2 diabetic subjects ( 2 ). NAFLD is viewed as a pathological spectrum with benign fatty liver at one end and nonalcoholic steatohepatitis (NASH; infl ammation, oxidative stress, hepatic damage, and fi brosis) at the other end ( 1 ). Thirty to 40% of individuals with simple fatty liver develop NASH, which can progress to cirrhosis, a risk factor for hepatic cancer ( 3 ).NAFLD involves excessive neutral lipid storage in the liver as TG, cholesteryl ester, and diacylglycerol (DAG) ( 4-6 ). NEFAs used for TG synthesis are derived from multiple sources including those mobilized from adipose tissue, Abstract Nonalcoholic fatty liver disease is a major public health concern in the obese and type 2 diabetic populations. Nonalcoholic fatty liver disease (NAFLD) is a signifi cant public health concern for the obese and type 2 diabetic

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“…In the present study, we did not find any increase in plasma FFA levels following 3 d of HFD intake either at rest or during the post-exercise recovery phase; however, it cannot be ruled out that the increased FFA levels were offset by enhanced utilization of FFAs as a fuel following adaptation to the HFD. Some previous studies reported that insulin resistance in skeletal muscle induced by an HFD is associated with lower phosphorylated levels of the insulin signaling cascade (19,32,33). However, others showed that the lower glucose uptake in post-exercise skeletal muscle after HFD consumption is not always accompanied by an impairment of the insulin signaling cascade (18,34).…”

Section: Discussionmentioning

confidence: 95%

“…Insulin resistance in skeletal muscle induced by an HFD is associated with lower phosphorylated levels of the insulin signaling cascade (19,32,33); conflicting results were observed in post-exercise skeletal muscle of mice which were provided HFD for 2 or 4 wk (18,34). We determined total amounts and phosphorylation states of proteins which were involved in glucose uptake and glycogen synthesis after post-exercise glucose administration.…”

Section: Levels and Phosphorylated States Of Proteins Involved In Insmentioning

confidence: 99%

Pre-Exercise High-Fat Diet for 3 Days Affects Post-Exercise Skeletal Muscle Glycogen Repletion

Takahashi

Matsunaga

Tamura

et al. 2017

J Nutr Sci Vitaminol

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Summary Previous studies have shown that the short-term intake of a high-fat diet (HFD) impairs glucose metabolism. In this study, we investigated the influences of pre-exercise HFD intake for 3 d on post-exercise glycogen repletion in skeletal muscle in ICR mice. Mice received either an HFD (57% kcal from fat, 23% kcal from carbohydrate; HFD group) or standard laboratory chow (13% kcal from fat, 60% kcal from carbohydrate; Con group) for 3 d before exercise. Mice performed treadmill running at 25 m/min for 60 min and were orally administered a glucose (2 mg/g body weight) solution immediately after and at 60 min after exercise. A negative main effect of pre-exercise HFD intake was observed for skeletal muscle glycogen concentration from the pre-exercise phase to 120 min of post-exercise recovery (p,0.01). Blood glucose concentration in the HFD group was significantly higher than in the Con group at 120 min after exercise (p,0.01). No significant difference was observed in plasma insulin concentration. There were no significant between-group differences in the phosphorylation state of Akt Thr308, AMPK Thr172, AS160 Thr642, or glycogen synthase Ser641 or in glucose transporter 4 protein levels during post-exercise recovery. Our results suggest that the intake of a pre-exercise HFD for 3 d affects post-exercise glycogen repletion in skeletal muscle without impairing the insulin signaling cascade. Key Words skeletal muscle, post-exercise recovery, high-fat diet, glucoseThe pre-exercise glycogen store in skeletal muscle is considered a major determinant of exercise performance during moderate-to high-intensity exercise (1-4). When exercise intensity exceeds around 60-65% VO2max, the skeletal muscle glycogen store is the predominant source of energy (5, 6). Moreover, recent studies suggest that glycogen per se is important for skeletal muscle force production by the modulating excitationcontraction coupling process (7). When individuals are involved in multiple training sessions and competitions over successive days or within a day, enhancing glycogen repletion in skeletal muscle after exercise is essential for improving performance during subsequent exercise.Some athletes alter their dietary composition of carbohydrate and fat for several days to enhance their exercise performance. For example, adaptation to a ketogenic diet, which severely restricts carbohydrate intake (,20 g/d or 5% of total daily energy intake) and contains high fat levels and an adequate amount of protein, has been shown to be useful for weight control without detrimental effects on skeletal muscle mass, power output, or strength after not only long-term (.2 wk) but also short-term (7 d) administration (8-10). Adaptation to a ketogenic diet increases fat utilization and reduces carbohydrate reliance during exercise without impairing endurance capacity at 62-64% VO 2max under a state of insufficient carbohydrate availability (overnight fasting) (11). However, as a ketogenic diet sometimes causes side effects, including increased perceived exertio...

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High-Fat Diet-Mediated Lipotoxicity and Insulin Resistance Is Related to Impaired Lipase Expression in Mouse Skeletal Muscle

Cited by 70 publications

References 63 publications

Defective Natriuretic Peptide Receptor Signaling in Skeletal Muscle Links Obesity to Type 2 Diabetes

Defective Natriuretic Peptide Receptor Signaling in Skeletal Muscle Links Obesity to Type 2 Diabetes

Fatty acid elongase-5 (Elovl5) regulates hepatic triglyceride catabolism in obese C57BL/6J mice

Pre-Exercise High-Fat Diet for 3 Days Affects Post-Exercise Skeletal Muscle Glycogen Repletion

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