A new fluorometric turn-on assay for alkaline phosphatase and inhibitor screening based on aggregation and deaggregation of tetraphenylethylene molecules

Gu, Xinggui; Zhang, Guanxin; Wang, Zhuo; Liu, Wenwen; Xiao, Le; Zhang, Deqing

doi:10.1039/c3an36784c

Cited by 97 publications

(49 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…34,35 Phosphate groups not only served as the substrate of ALP, but also rendered the probe water-soluble and almost non-emissive. In this regard, AIE probes with phosphate groups were developed for sensing of alkaline phosphatase (ALP).…”

Section: Aie-small Molecule Conjugatesmentioning

confidence: 99%

Specific light-up bioprobes based on AIEgen conjugates

2015

View full text Add to dashboard Cite

Driven by the high demand for sensitive and specific tools for optical sensing and imaging, bioprobes with various working mechanisms and advanced functionalities are flourishing at an incredible speed. Conventional fluorescent probes suffer from the notorious effect of aggregation-caused quenching that imposes limitation on their labelling efficiency or concentration to achieve desired sensitivity. The recently emerged fluorogens with an aggregation-induced emission (AIE) feature offer a timely remedy to tackle the challenge. Utilizing the unique properties of AIE fluorogens (AIEgens), specific light-up probes have been constructed through functionalization with recognition elements, showing advantages such as low background interference, a high signal to noise ratio and superior photostability with activatable therapeutic effects. In this tutorial review, we summarize the recent progress in the development of specific AIEgen-based light-up bioprobes. Through illustration of their operation mechanisms and application examples, we hope to provide guidelines for the design of more advanced AIE sensing and imaging platforms with high selectivity, great sensitivity and wide adaptability to a broad range of biomedical applications.

show abstract

Section: Aie-small Molecule Conjugatesmentioning

confidence: 99%

Specific light-up bioprobes based on AIEgen conjugates

2015

View full text Add to dashboard Cite

show abstract

“…For most AIE probes, a high signal-to-background ratio was realized. Though the ALP turnon probes based on the phosphorylated TPE derivatives were reported previously, 31,32 their applications in detecting the ALP activity in living cells, especially for stem cells, are still limited and remain to be explored.…”

Section: Introductionmentioning

confidence: 99%

Fluorescence light-up AIE probe for monitoring cellular alkaline phosphatase activity and detecting osteogenic differentiation

et al. 2016

View full text Add to dashboard Cite

Alkaline phosphatase (ALP) is an important monophosphate hydrolase during cell mineralization and osteogenic differentiation. Though traditional methods are provided for evaluating the ALP expression in the fixed and lysed cells, at present it is still challenging to monitor the ALP activity in living cells. In this work, three phosphorylated tetraphenylethylene (TPE) probes (TPE-PA, TPE-2PA and TPE-4PA) with different numbers of -PO 3 H 2 groups were synthesized for monitoring the ALP activity. It was found that in aqueous solution, both the TPE-PA and TPE-2PA probes were highly sensitive to ALP. In the presence of ALP, they could be quickly hydrolysed, resulting in an aggregation-induced emission (AIE) to light up ALP. While in living cells, only TPE-2PA showed good cell penetrability and high fluorescence signal-tonoise ratio during osteogenic differentiation. This probe provides us a new strategy to screen the ALP activity in living stem cells for detecting osteogenic differentiation.

show abstract

“…In fact, the development of convenient sensors for monitoring ALP activity is extremely important and valuable not only for clinical diagnoses but also for biomedical research. We utilized TPE‐PO 3 H 2 (Scheme ) to construct a new fluorometric turn‐on assay for ALP activity and inhibitor screening 43. As shown in Figure , TPE‐PO 3 H 2 was rather weakly emissive before incubation with ALP.…”

Section: Bio‐/chemosensors With Functionalized Aie Moleculesmentioning

confidence: 99%

Bio‐/Chemosensors and Imaging with Aggregation‐Induced Emission Luminogens

Zhan

You

Zhang

et al. 2016

The Chemical Record

Self Cite

View full text Add to dashboard Cite

Aggregation-induced emission (AIE) luminogens show abnormal fluorescent behavior; they are non-emissive in solution, but they become strongly emissive after aggregation. Sensing and imaging are the major applications of AIE luminogens. By properly manipulating the aggregation and deaggregation of AIE molecules, various bio-/chemosensors have been developed. Moreover, AIE molecules with targeting groups have been devised for imaging of organelles and cancer cells. In this account, we report our recent work on the application of AIE luminogens for the construction of bio-/chemosensors and imaging.

show abstract

A new fluorometric turn-on assay for alkaline phosphatase and inhibitor screening based on aggregation and deaggregation of tetraphenylethylene molecules

Cited by 97 publications

References 41 publications

Specific light-up bioprobes based on AIEgen conjugates

Specific light-up bioprobes based on AIEgen conjugates

Fluorescence light-up AIE probe for monitoring cellular alkaline phosphatase activity and detecting osteogenic differentiation

Bio‐/Chemosensors and Imaging with Aggregation‐Induced Emission Luminogens

Contact Info

Product

Resources

About