Mechanism of the uptake of cationic and anionic calcium phosphate nanoparticles by cells

Sokolova, Viktoriya; Kozlova, Diana; Knuschke, Torben; Buer, Jan; Westendorf, Astrid M.; Epple, Matthias

doi:10.1016/j.actbio.2013.02.034

Cited by 115 publications

(88 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Low temperatures inhibit biological processes and have been shown to block the internalization of NPs. 44 The increase in fluorescence was largely blocked by incubation of the cells on ice, suggesting that FITC-Ag@SiO2 uptake into BMDC is an active biological process. The slight increase in fluorescence seen in cells incubated with NPs on ice compared to cells without NPs suggested that a small amount of nanocontainers adhere passively to the cell surface.…”

Section: Uptake Of Sio 2 Nanocontainers Into Immune Cellsmentioning

confidence: 98%

Antimicrobial silver-filled silica nanorattles with low immunotoxicity in dendritic cells

Priebe

Widmer

Löwa

et al. 2017

Nanomedicine: Nanotechnology, Biology and Medicine

View full text Add to dashboard Cite

The progression in the use of orthopedic implants has led to an increase in the absolute number of implant infections, triggering a search for more effective antibacterial coatings. Nanorattles have recently gained interest in biomedical applications such as drug delivery, as encapsulation of the cargo inside the hollow structure provides a physical protection from the surrounding environment. Here, silvercontaining silica nanorattles (Ag@SiO 2 ) were evaluated for their antimicrobial potential and for their impact on cells of the immune system. We show that Ag@SiO 2 nanorattles exhibited a clear antibacterial effect against Escherichia coli as well as Staphylococcus aureus found in post-operative infections. Immunotoxicological analyses showed that the particles were taken up through an active phagocytic process by dendritic cells of the immune system and did not affect their viability nor induce unwanted immunological effects. Silver-containing silica nanorattles thus fulfill several prerequisites for an antibacterial coating on surgical implants.

show abstract

Section: Uptake Of Sio 2 Nanocontainers Into Immune Cellsmentioning

confidence: 98%

Antimicrobial silver-filled silica nanorattles with low immunotoxicity in dendritic cells

Priebe

Widmer

Löwa

et al. 2017

Nanomedicine: Nanotechnology, Biology and Medicine

View full text Add to dashboard Cite

show abstract

“…2A and B). NP were also exposed to J774 macrophages for 24 h at 4°C, a temperature at which many active intracellular processes are decreased (Sokolova et al, 2013). At this temperature, the amount of DiO-positive cells was strongly reduced for both NPa and NPb, indicating that a major part of the NP is actively taken up at 37°C.…”

Section: Np Are Taken Up By a Macrophage Cell Linementioning

confidence: 99%

Polymer-based nanoparticles loaded with a TLR7 ligand to target the lymph node for immunostimulation

Widmer

Thauvin

Mottas

et al. 2018

International Journal of Pharmaceutics

View full text Add to dashboard Cite

A B S T R A C TSmall-molecule agonists for the Toll-like receptors (TLR) 7 and 8 are effective for the immunotherapy of skin cancer when used as topical agents. Their systemic use has however been largely unsuccessful due to doselimiting toxicity. We propose a polymer-based nanodelivery system to target resiquimod, a TLR7 ligand, to the lymph node in order to focus the immunostimulatory activity and to prevent a generalized inflammatory response. We demonstrate successful encapsulation of resiquimod in methoxypoly(ethylene glycol)-b-poly(DLlactic acid) (mPEG-PLA) and mixed poly(DL-lactic-co-glycolic acid) (PLGA)/mPEG-PLA nanoparticles. We show that these particles are taken up mainly by dendritic cells and macrophages, which are the prime initiators of anticancer immune responses. Nanoparticles loaded with resiquimod activate these cells, demonstrating the availability of the immune-stimulating cargo. The unloaded particles are non-inflammatory and do not have cytotoxic activity on immune cells. Following subcutaneous injection in mice, mPEG-PLA and PLGA/mPEG-PLA nanoparticles are detected in dendritic cells and macrophages in the draining lymph nodes, demonstrating the targeting potential of these particles. Thus, polymer-based nanoparticles represent a promising delivery system that allows lymph node targeting for small-molecule TLR7 agonists in the context of systemic cancer immunotherapy.

show abstract

“…[22] Attachment of particles to the specific site of cells can be tuned not only via surface chemistry but also by changing the geometry of the particles even at the nanoscale. [23], [24], [25] Thus, future trends in particle assisted drug delivery are seen towards novel methods of synthesis and elaboration of anisotropic particles which in some cases may even resemble such natural entities as bacteria. [26], [27] The flexibility of polyelectrolytes allows keeping different shapes of the formed multilayer capsules after decomposition of the sacrificial solid templates.…”

Section: Anisotropic Shapesmentioning

confidence: 99%

Release from Polyelectrolyte Multilayer Capsules in Solution and on Polymeric Surfaces

Parakhonskiy

Yashchenok

Möhwald

et al. 2016

Adv Materials Inter

View full text Add to dashboard Cite

Mechanism of the uptake of cationic and anionic calcium phosphate nanoparticles by cells

Cited by 115 publications

References 40 publications

Antimicrobial silver-filled silica nanorattles with low immunotoxicity in dendritic cells

Antimicrobial silver-filled silica nanorattles with low immunotoxicity in dendritic cells

Polymer-based nanoparticles loaded with a TLR7 ligand to target the lymph node for immunostimulation

Release from Polyelectrolyte Multilayer Capsules in Solution and on Polymeric Surfaces

Contact Info

Product

Resources

About