Copious urinary excretion of a male Syrian hamster (Mesocricetus auratus) salivary gland protein after its endocrine-like release upon β-adrenergic stimulation

Dubey, Ved Prakash; Srikantan, Subramanya; Mohammad, Mahabub Pasha; Rajan, Wenson D.; De, Prabir

doi:10.1016/j.ygcen.2013.02.016

Cited by 1 publication

(7 citation statements)

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“…MSP lipocalin is known to be present in the SMG, saliva and urine of male Golden hamsters as the N-glycosylated 24-kDa and non-glycosylated 20.5-kDa major forms, along with a minor, higher N-glycosylated form of approximately 30 kDa [13,14,15]. As the IgE-reactive bands detected in Golden hamster fur corresponded to the molecular weight expected for MSP, polyclonal rabbit antibodies raised against MSP were used to detect MSP in the different fur extracts (fig.…”

Section: Resultsmentioning

confidence: 99%

“…Figure 3a shows nMSP purified from SMG containing glycosylated (24 and 30 kDa) and non-glycosylated (20.5-kDa) forms [13,14,15] and rMSP purified after expression in E. coli . The sera of all patients except P1 reacted with recombinant and native MSP (fig.…”

Section: Resultsmentioning

confidence: 99%

“…The submaxillary gland (SMG) extracts of male Golden hamsters were prepared by homogenization (2.5% w/v) in chilled 20 m M Tris-HCl (pH 7.4) and centrifugation at 28,000 g for 30 min at 4°C as described [12,15]. Golden hamsters were handled according to the Centre's Institutional Animal Experimentation Ethics guidelines.…”

Section: Methodsmentioning

confidence: 99%

“…Natural MSP (nMSP) lipocalins present in adult male Golden hamster SMG extracts (as 24-kDa, 20.5-kDa and 30-kDa forms, which differ in the presence or absence of N-glycosylation [13,14,15]) were purified employing a rapid procedure. The proteins precipitated from the extract within an 85-100% (NH 4 ) 2 SO 4 saturation were collected by centrifugation, dissolved in 20 m M Tris-HCl containing 150 m M NaCl (pH 7.4) and resolved in a gel-filtration column (1.6 × 95 cm; Sephadex-G75 superfine; GE Healthcare, Buckinghamshire, UK) equilibrated with the same buffer.…”

Section: Methodsmentioning

confidence: 99%

“…In the Golden hamster, a male-specific submaxillary gland protein (MSP) has been identified, which is secreted in the saliva and excreted in the urine of males. MSP, which exists in glycosylated as well as non-glycosylated forms, is a well-characterized lipocalin, having the closest identity with the odorant-binding lipocalins [12,13,14,15]. Low levels of MSP are also expressed female-specifically in the lacrimal glands and secreted in the tears of females [14].…”

Section: Introductionmentioning

confidence: 99%

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Male-Specific Submaxillary Gland Protein, a Lipocalin Allergen of the Golden Hamster, Differs from the Lipocalin Allergens of Siberian and Roborovski Dwarf Hamsters

Hilger

Dubey

Lentz

et al. 2015

Int Arch Allergy Immunol

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Background: An increasing number of asthma cases upon exposure to hamsters and anaphylactic reactions following hamster bites are being reported, but the allergens responsible are still poorly characterized. In the Golden hamster, male-specific submaxillary gland protein (MSP), a lipocalin expressed in a sex- and tissue-specific manner in the submaxillary and lacrimal glands, is secreted in the saliva, tears and urine. The purpose of this study was to determine if MSP is an allergen, to identify IgE-reactive proteins of different hamster species and to analyse potential cross-reactivities. Methods: Fur extracts were prepared from four hamster species. Hamster-allergic patients were selected based on a history of positive IgE-test to hamster epithelium. The IgE-reactivity of patients' sera was investigated by means of immunoblot and ELISA. IgE-reactive proteins in fur extracts and the submaxillary gland were identified using anti-MSP antibodies, Edman sequencing or mass spectrometry. MSP was purified from Golden hamster and recombinant MSP was expressed in E. coli. Results: Four patients had IgE-antibodies against 20.5-kDa and 24-kDa proteins of Golden hamster fur extract, which were identified as MSP. IgE-reactive MSP-like proteins were detected in European hamster fur extract. Three patient sera showed IgE-reactive bands at 17-21 kDa in Siberian and Roborovski hamster fur extracts. These proteins were identified as two closely related lipocalins. Immunoblot inhibition experiments showed that they are cross-reactive and are different from MSP. Conclusion: MSP lipocalin of the Golden hamster was identified as an allergen, and it is different from the cross-reactive lipocalin allergens of Siberian and Roborovski hamsters. Our findings highlight the need for specific tools for the in vitro and in vivo diagnosis of allergy to different hamster species.

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Section: Resultsmentioning

confidence: 99%