Antibiofilm activity, pH and solubility of endodontic sealers

Faria-Júnior, Norberto Batista de; Tanomaru-Filho, Mário; Berbert, Fábio Luiz Camargo Villela; Guerreiro-Tanomaru, Juliane Maria

doi:10.1111/iej.12055

Cited by 98 publications

(127 citation statements)

References 32 publications

(60 reference statements)

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“…Sealapex is a calcium hydroxide-based sealer that showed excellent antimicrobial activity in periods of 24 h, 48 h, 72 h and 7 days by agar diffusion. 13,14,15,16,21 Similar results were found by Faria-Júnior et al 19 using DCT against E. faecalis biofilm. Heyder et al…”

Section: Discussionsupporting

confidence: 78%

“…21 The Direct Contact Test (DCT) was chosen in the present study, because it was designed to overcome these limitations, to enable assessment of the in vitro antibacterial activities of numerous endodontic sealers. 19,20,22 Moreover, direct contact with the biofilm grown on dentin blocks avoids false positives attributed to physical entombment of bacteria in root canals and dentinal tubules.…”

Section: Discussionmentioning

confidence: 99%

“…In this research, E. faecalis was allowed to grow over bovine dentine blocks for 14 days to form biofilm, following the method described by Faria-Júnior et al 19 Previous studies have used different biofilm induction periods. 11,20,23 However, it has been shown that biofilms formed in the first few days of incubation may not display the same antimicrobial resistance of a mature biofilm.…”

Section: Discussionmentioning

confidence: 99%

“…The cell culture plates with the submerged bovine dentine blocks were kept in 5% CO 2 (Ultra Safe, HF212-UV) at 37°C for 14 days. 19,20 The BHI medium of each specimen was completely changed every 48 h without adding new microorganisms.…”

Section: Analysis Of Antibiofilm Activitymentioning

confidence: 99%

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Antimicrobial action of calcium hydroxide-based endodontic sealers after setting, against E. faecalis biofilm

et al. 2016

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Antimicrobial action of calcium hydroxide-based endodontic sealers after setting, against E. faecalis biofilm Abstract: Enterococcus faecalis are gram positive bacteria that can mostly resist endodontic therapy, inducing persistent infection in the root canal system. Endodontic sealers with antimicrobial activity may help eliminate residual microorganisms that survive endodontic treatment. The present study aimed at comparing the antimicrobial activity of Acroseal, Sealapex and AH Plus endodontic sealers in an in vitro biofilm model. Bovine dentin specimens (144) were prepared, and twelve blocks for each sealer and each experimental time point (2, 7 and 14 days) were placed and left in contact with plates containing inoculum of E. faecalis (ATCC 51299), to induce biofilm formation. After 14 days, the samples were transferred to another plate with test sealers and kept at 37°C and 5% CO 2 for 2, 7 and 14 days. The specimens without sealers were used as a control for each period. The samples were agitated in a sonicator after each experiment. The suspensions were agitated in a vortex mixer, serially diluted in saline, and triple plated onto m-Enterococcus agar. Colonyforming units were counted, and the data were statistically analyzed using ANOVA, Shapiro-Wilk and Kruskal-Wallis one-way tests (p < 0.05) to determine antimicrobial potential. Sealapex showed significant differences at all the experimental time points, in comparison with all the other groups. AH Plus and Acroseal showed antimicrobial activity only on the 14 th experimental day. Neither of the sealers tested were able to completely eliminate the biofilm. Sealapex showed the highest antimicrobial activity in all the experimental periods. The antimicrobial activity of all the sealers analyzed increased over time.

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Section: Discussionsupporting

confidence: 78%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Analysis Of Antibiofilm Activitymentioning

confidence: 99%

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Antimicrobial action of calcium hydroxide-based endodontic sealers after setting, against E. faecalis biofilm

et al. 2016

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“…1,3 Moreover, endodontic sealers presenting antibacterial activity are desirable for suppressing bacterial growth and preventing biofilm development. 4 …”

mentioning

confidence: 99%

In vitro antibacterial activity of a silicone-based endodontic sealer and two conventional sealers

et al. 2016

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In vitro antibacterial activity of a silicone-based endodontic sealer and two conventional sealers Abstract: The aim of this study was to evaluate whether the modification in the silver component is capable of providing GuttaFlow 2 with antibacterial activity against Enterococcus faecalis compared with epoxy resin-based (AH Plus) and zinc oxide and eugenol-based (Endofill) sealers. The antibacterial activity was evaluated using a reference strain of E. faecalis (ATCC 29212). Freshly mixed sealers were subjected to the agar diffusion test (ADT), while the direct contact test (DCT) was performed after materials setting. ADT results were obtained through measurements, in millimeters, of the inhibition zones promoted by the materials, using a digital caliper. In DCT, values of CFU/mL promoted by the three sealers were compared in three experimental periods (1 min, 1 h, and 24 h). The data were analyzed using Kruskal-Wallis and Dunn post-hoc tests (p < 0.05). In both ADT and DCT, GuttaFlow 2 presented no effect against E. faecalis, while Endofill and AH Plus showed similar inhibition zones. Endofill was the only material capable of reducing bacterial growth in DCT. In conclusion, modifications in the silver particle of GuttaFlow 2 did not result in a sealer with antibacterial effect against E. faecalis.

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Root canal filling

Jain¹,

Yeli

Dhingra³

2016

Current Therapy in Endodontics

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Antibiofilm activity, pH and solubility of endodontic sealers

Abstract: Sealapex and MTA-F were associated with a reduction in the number of bacteria in biofilms and had greater solubility. The high solubility and pH may be related to the antibacterial activity of these materials.

Cited by 98 publications

References 32 publications

Antimicrobial action of calcium hydroxide-based endodontic sealers after setting, against E. faecalis biofilm

Antimicrobial action of calcium hydroxide-based endodontic sealers after setting, against E. faecalis biofilm

In vitro antibacterial activity of a silicone-based endodontic sealer and two conventional sealers

Root canal filling

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