Quantum Yield Measurement in a Multicolor Chromophore Solution Using a Nanocavity

Chizhik, Alexey I.; Gregor, Ingo

doi:10.1021/nl400313z

Cited by 25 publications

(27 citation statements)

References 20 publications

(31 reference statements)

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“…The PL efficiencies for ZnS:Mn-MAA NCs were measured and calculated following the method originally reported by Williams et al . [ 29 , 30 ]. This method includes calculating a relative quantum yield by comparing it to that of a standard organic dye, a 0.1 M solution of quinine sulfate in H 2 SO 4 (purchased from Fluka) in this case [ 31 ], of which the emission wavelength and absolute quantum efficiency are known as 550 nm and 0.546, respectively (22 °C).…”

Section: Resultsmentioning

confidence: 99%

Surface Properties and Photocatalytic Activities of the Colloidal ZnS:Mn Nanocrystals Prepared at Various pH Conditions

Heo

Hwang

2015

Nanomaterials

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Water-dispersible ZnS:Mn nanocrystals (NC) were synthesized by capping the surface with mercaptoacetic acid (MAA) molecules at three different pH conditions. The obtained ZnS:Mn-MAA NC products were physically and optically characterized by corresponding spectroscopic methods. The UV-Visible absorption spectra and PL emission spectra showed broad peaks at 310 and 590 nm, respectively. The average particle sizes measured from the HR-TEM images were 5 nm, which were also supported by the Debye-Scherrer calculations using the X-ray diffraction (XRD) data. Moreover, the surface charges and the degrees of aggregation of the ZnS:Mn-MAA NCs were determined by electrophoretic and hydrodynamic light scattering methods, indicating formation of agglomerates in water with various sizes (50–440 nm) and different surface charge values accordingly the preparation conditions of the NCs (−7.59 to −24.98 mV). Finally, the relative photocatalytic activities of the ZnS:Mn-MAA NCs were evaluated by measuring the degradation rate of methylene blue (MB) molecule in a pseudo first-order reaction condition under the UV-visible light irradiation. As a result, the ZnS:Mn-MAA NC prepared at the pH 7 showed the best photo-degradation efficiency of the MB molecule with the first-order rate constant (kobs) of 2.0 × 10−3·min−1.

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Section: Resultsmentioning

confidence: 99%

Surface Properties and Photocatalytic Activities of the Colloidal ZnS:Mn Nanocrystals Prepared at Various pH Conditions

Heo

Hwang

2015

Nanomaterials

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“…An alternative way that allows one to modulate the radiative rate of fluorophores while keeping them freely floating in buffer is placing a droplet of solution between two metallic mirrors and tuning the distance between them. We have used this, so-called plasmonic nanocavity-based method, for measuring QY of dye molecules 18 – 21 and semiconductor nanocrystals 22 , 23 .…”

Section: Introductionmentioning

confidence: 99%

“…As the method is based exclusively on the measurement of the excited-state lifetime modulation, it is not prone to underestimating the QY due to light absorption by non-radiative species. The method can be used for any type of electric dipole emitter and was proven to be a reliable tool for determining QYs of dye molecules 19 , semiconductor nanocrystals 23 , or carbon nanodots 28 . It also allowed us to measure QYs of emitters in highly complex systems, such as mixtures of different types of fluorophores in liquid and solid states with overlapping absorption and fluorescence spectra 22 , 23 .…”

Section: Introductionmentioning

confidence: 99%

“…The method can be used for any type of electric dipole emitter and was proven to be a reliable tool for determining QYs of dye molecules 19 , semiconductor nanocrystals 23 , or carbon nanodots 28 . It also allowed us to measure QYs of emitters in highly complex systems, such as mixtures of different types of fluorophores in liquid and solid states with overlapping absorption and fluorescence spectra 22 , 23 . The high sensitivity of the technique allows one even to measure QYs of molecules inside single supported lipid bilayers 21 , or even of individual fluorophores 18 .…”

Section: Introductionmentioning

confidence: 99%

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Absolute quantum yield measurements of fluorescent proteins using a plasmonic nanocavity

et al. 2020

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One of the key photophysical properties of fluorescent proteins that is most difficult to measure is the quantum yield. It describes how efficiently a fluorophore converts absorbed light into fluorescence. Its measurement using conventional methods become particularly problematic when it is unknown how many of the proposedly fluorescent molecules of a sample are indeed fluorescent (for example due to incomplete maturation, or the presence of photophysical dark states). Here, we use a plasmonic nanocavity-based method to measure absolute quantum yield values of commonly used fluorescent proteins. The method is calibration-free, does not require knowledge about maturation or potential dark states, and works on minute amounts of sample. The insensitivity of the nanocavity-based method to the presence of non-luminescent species allowed us to measure precisely the quantum yield of photo-switchable proteins in their on-state and to analyze the origin of the residual fluorescence of protein ensembles switched to the dark state.

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“…[91]. An elegant absolute and calibrationfree technique of measuring quantum yields of single emitters is to measure the modulation of the fluorescence emission rate in the vicinity of a planar metallic interface [92][93][94][95][96][97][98][99][100][101][102][103].…”

Section: Quantum Efficiencymentioning

confidence: 99%

Super-resolution imaging: when biophysics meets nanophotonics

et al. 2021

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Probing light–matter interaction at the nanometer scale is one of the most fascinating topics of modern optics. Its importance is underlined by the large span of fields in which such accurate knowledge of light–matter interaction is needed, namely nanophotonics, quantum electrodynamics, atomic physics, biosensing, quantum computing and many more. Increasing innovations in the field of microscopy in the last decade have pushed the ability of observing such phenomena across multiple length scales, from micrometers to nanometers. In bioimaging, the advent of super-resolution single-molecule localization microscopy (SMLM) has opened a completely new perspective for the study and understanding of molecular mechanisms, with unprecedented resolution, which take place inside the cell. Since then, the field of SMLM has been continuously improving, shifting from an initial drive for pushing technological limitations to the acquisition of new knowledge. Interestingly, such developments have become also of great interest for the study of light–matter interaction in nanostructured materials, either dielectric, metallic, or hybrid metallic-dielectric. The purpose of this review is to summarize the recent advances in the field of nanophotonics that have leveraged SMLM, and conversely to show how some concepts commonly used in nanophotonics can benefit the development of new microscopy techniques for biophysics. To this aim, we will first introduce the basic concepts of SMLM and the observables that can be measured. Then, we will link them with their corresponding physical quantities of interest in biophysics and nanophotonics and we will describe state-of-the-art experiments that apply SMLM to nanophotonics. The problem of localization artifacts due to the interaction of the fluorescent emitter with a resonant medium and possible solutions will be also discussed. Then, we will show how the interaction of fluorescent emitters with plasmonic structures can be successfully employed in biology for cell profiling and membrane organization studies. We present an outlook on emerging research directions enabled by the synergy of localization microscopy and nanophotonics.

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Quantum Yield Measurement in a Multicolor Chromophore Solution Using a Nanocavity

Cited by 25 publications

References 20 publications

Surface Properties and Photocatalytic Activities of the Colloidal ZnS:Mn Nanocrystals Prepared at Various pH Conditions

Surface Properties and Photocatalytic Activities of the Colloidal ZnS:Mn Nanocrystals Prepared at Various pH Conditions

Absolute quantum yield measurements of fluorescent proteins using a plasmonic nanocavity

Super-resolution imaging: when biophysics meets nanophotonics

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