Selection of perforin expressing CD4+ adenovirus-specific T-cells with artificial antigen presenting cells

Adenovirus Infections in Immunocompetent and Immunocompromised Patients

“…Short-term in vitro expansion under good manufacturing practice (GMP) conditions can render adoptive T-cell transfer available in less than 2 weeks (123,(252)(253)(254). In addition to a variety of methods based on the isolation of HAdV-specific T cells from the original stem cell donors, several approaches exploiting third-party donors are emerging (141,(255)(256)(257)(258)(259)(260)(261)(262)(263). Allogeneic third-party donors are a particularly important alternative option for cord blood recipients, for patients receiving allografts from HAdV-seronegative donors, and for solid organ transplantations from cadaveric donors, where donor blood is not available.…”

Antiadenovirus drug discovery: potential targets and evaluation methodologies

“…The use of specific memory T cells has also been shown to be safe and effective for treating HAdV infections, especially in pediatric and adult patients receiving HSCT [33,[36][37][38][39]. Haveman et al demonstrated that CD4+ T cells are crucial for controlling HAdV infection, and developed a method to expand and isolate functional HAdV-specific CD4+ T cells from peripheral blood mononuclear cells (PBMCs) that were not alloreactive and killed HAdV-infected cells effectively [40].…”

Oncolytic Adenoviruses in Cancer Treatment

“…Activated DCs will in turn traffic to lymph nodes to activate antiviral CD4 and CD8 T cells as part of the adaptive response. The major part of the cellular response consists of CD4+ T cells with both helper and CTL capabilities [44,45]. The clinical experience with adenovirus and other viruses on virotherapy indicates that this virus-mediated innate inflammation and antiviral T cell infiltration occur and are able to clear the virus injected in tumors.…”