Molecular properties of the class III subfamily of acyl-coenyzme A binding proteins from tung tree (Vernicia fordii)

Pastor, Steven; Sethumadhavan, Kandan; Ullah, Abul H. J.; Gidda, Satinder K.; Cao, Heping; Mason, Catherine B.; Chapital, Dorselyn C.; Scheffler, Brian E.; Mullen, Robert T.; Dyer, John M.; Shockey, Jay

doi:10.1016/j.plantsci.2012.12.009

Cited by 23 publications

(19 citation statements)

References 43 publications

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“…Anonymous tung seed cDNA sequences from week 6 were generated and analyzed by pyrosequencing (“454”) technology as described previously [19]. Comparisons of this dataset to existing databases using BLAST revealed a partial sequence highly similar to the sequence coding for the soluble isoform of DGAT from peanut (AhDGAT3 [29]).…”

Section: Resultsmentioning

confidence: 99%

“…The 3′ portion of the tung DGAT3 gene was identified from double-stranded cDNA samples prepared from mid-development (time point 6) tung seeds, as described previously [19]. The remaining sequence at the 5′ end of the open-reading frame was determined by two rounds of adaptor anchored PCR from tung genomic DNA, as described by the manufacturer (GenomeWalker kit, Clontech), using the provided adaptor primers and the gene-specific primers VfDGAT3-5GW1 (5′-GACCCTGACATTAGGACCGTCCCTG-3′) and VfDGAT3-5GW2 (5′-CCCTGCACTTGCCCATACACTTGCA-3′).…”

Section: Methodsmentioning

confidence: 99%

“…These genes code for diacylglycerol acyltransferase 1 and 2 (DGAT1 and DGAT2) [16], delta-12 oleic acid desaturase (FAD2) [17], delta-12 fatty acid conjugase (FADX) [17], omega-3 fatty acid desaturase (FAD3) [18], acyl-CoA binding proteins (ACBPs) [19], cytochrome b5 isoforms (Cb5s) [20], cytochrome b5 reductase [21], glycerol-3-phosphate acyltransferases (GPATs) [22], aquaporin [23], glutaredoxin [23] and oleosins [24].…”

Section: Introductionmentioning

confidence: 99%

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Developmental Regulation of Diacylglycerol Acyltransferase Family Gene Expression in Tung Tree Tissues

et al. 2013

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Diacylglycerol acyltransferases (DGAT) catalyze the final and rate-limiting step of triacylglycerol (TAG) biosynthesis in eukaryotic organisms. DGAT genes have been identified in numerous organisms. Multiple isoforms of DGAT are present in eukaryotes. We previously cloned DGAT1 and DGAT2 genes of tung tree (Vernicia fordii), whose novel seed TAGs are useful in a wide range of industrial applications. The objective of this study was to understand the developmental regulation of DGAT family gene expression in tung tree. To this end, we first cloned a tung tree gene encoding DGAT3, a putatively soluble form of DGAT that possesses 11 completely conserved amino acid residues shared among 27 DGAT3s from 19 plant species. Unlike DGAT1 and DGAT2 subfamilies, DGAT3 is absent from animals. We then used TaqMan and SYBR Green quantitative real-time PCR, along with northern and western blotting, to study the expression patterns of the three DGAT genes in tung tree tissues. Expression results demonstrate that 1) all three isoforms of DGAT genes are expressed in developing seeds, leaves and flowers; 2) DGAT2 is the major DGAT mRNA in tung seeds, whose expression profile is well-coordinated with the oil profile in developing tung seeds; and 3) DGAT3 is the major form of DGAT mRNA in tung leaves, flowers and immature seeds prior to active tung oil biosynthesis. These results suggest that DGAT2 is probably the major TAG biosynthetic isoform in tung seeds and that DGAT3 gene likely plays a significant role in TAG metabolism in other tissues. Therefore, DGAT2 should be a primary target for tung oil engineering in transgenic organisms.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Developmental Regulation of Diacylglycerol Acyltransferase Family Gene Expression in Tung Tree Tissues

et al. 2013

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“…Identification and characterization of all genes involved in tung oil biosynthesis is essential for improving tung oil production and economic value. Many tung oil biosynthetic genes have been identified in our laboratories, including those coding for diacylglycerol acyltransferases ( DGAT ) [13, 17], delta-12 oleic acid desaturase ( FAD2 ) and delta-12 fatty acid conjugase ( FADx ) [14], omega-3 fatty acid desaturase ( FAD3 ) [41], acyl-CoA binding proteins [42], cytochrome b5 [43], cytochrome b5 reductase [15], glycerol-3-phosphate acyltransferase ( GPAT ) [44], plastid-type omega-3 fatty acid desaturase ( TnDES2 ) [45], aquaporin and glutaredoxin [46], and ß-ketoacyl-ACP synthase ( KAS ) [47]. Interestingly, we identified an additional FADx gene, FADx-2 , which was probably generated by gene duplication and sub-functionalization based on the different expression patterns of FADx-1 and FADx-2 genes.…”

Section: Discussionmentioning

confidence: 99%

The Tung Tree (Vernicia Fordii) Genome Provides A Resource for Understanding Genome Evolution and Oil Improvement

Zhang

Liu

Long

et al. 2019

Preprint

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“…The immediate challenge of post-genomic biology is to determine the biological functions of proteins encoded by unknown genes. Approaches to gain clues about the structure-function relationship of proteins are to perform comprehensive amino acid sequence analysis (Cao, 2011), predict their structures at the secondary and three-dimensional levels (Hyun et al, 2013;Pastor et al, 2013), and solve the structures of important proteins 2002;Wang and Tanaka Hall, 2001).…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Analysis of Oleosin Gene Family in 22 Tree Species: An Accelerator for Metabolic Engineering of BioFuel Crops and Agrigenomics Industrial Applications?

Cao

2015

OMICS: A Journal of Integrative Biology

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Trees contribute to enormous plant oil reserves because many trees contain 50%-80% of oil (triacylglycerols, TAGs) in the fruits and kernels. TAGs accumulate in subcellular structures called oil bodies/droplets, in which TAGs are covered by low-molecular-mass hydrophobic proteins called oleosins (OLEs). The OLEs/TAGs ratio determines the size and shape of intracellular oil bodies. There is a lack of comprehensive sequence analysis and structural information of OLEs among diverse trees. The objectives of this study were to identify OLEs from 22 tree species (e.g., tung tree, tea-oil tree, castor bean), perform genome-wide analysis of OLEs, classify OLEs, identify conserved sequence motifs and amino acid residues, and predict secondary and threedimensional structures in tree OLEs and OLE subfamilies. Data mining identified 65 OLEs with perfect conservation of the ''proline knot'' motif (PX5SPX3P) from 19 trees. These OLEs contained >40% hydrophobic amino acid residues. They displayed similar properties and amino acid composition. Genome-wide phylogenetic analysis and multiple sequence alignment demonstrated that these proteins could be classified into five OLE subfamilies. There were distinct patterns of sequence conservation among the OLE subfamilies and within individual tree species. Computational modeling indicated that OLEs were composed of at least three ahelixes connected with short coils without any b-strand and that they exhibited distinct 3D structures and ligand binding sites. These analyses provide fundamental information in the similarity and specificity of diverse OLE isoforms within the same subfamily and among the different species, which should facilitate studying the structurefunction relationship and identify critical amino acid residues in OLEs for metabolic engineering of tree TAGs.

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Molecular properties of the class III subfamily of acyl-coenyzme A binding proteins from tung tree (Vernicia fordii)

Cited by 23 publications

References 43 publications

Developmental Regulation of Diacylglycerol Acyltransferase Family Gene Expression in Tung Tree Tissues

Developmental Regulation of Diacylglycerol Acyltransferase Family Gene Expression in Tung Tree Tissues

The Tung Tree (Vernicia Fordii) Genome Provides A Resource for Understanding Genome Evolution and Oil Improvement

Genome-Wide Analysis of Oleosin Gene Family in 22 Tree Species: An Accelerator for Metabolic Engineering of BioFuel Crops and Agrigenomics Industrial Applications?

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