Fractionation of Phenolic Compounds Extracted from Propolis and Their Activity in the Yeast Saccharomyces cerevisiae

Petelinc, Tanja; Polak, Tomaž; Demšar, Lea; Jamnik, Polona

doi:10.1371/journal.pone.0056104

Cited by 18 publications

(27 citation statements)

References 23 publications

(20 reference statements)

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“…Therefore, the authors concluded that the phenolic profile of Slovenian propolis is comparable to that of European propolis. [51,52] More recently, a group of research has reported that propolis samples from Slovenia, Croatia and France, mainly reflected patterns characteristic of blue-type propolis rich in flavonoids and with low quantity of phenolic acids. [53] Ethanolic extracts of propolis from several locations in Croatia, analyzed by several methods such as GC/ MS, two-dimensional thin-layer chromatography, HPLC-thin-layer chromatography among others, had relatively high amounts of flavonoids besides the presence of phenolic acids.…”

Section: Southern European Coastmentioning

confidence: 99%

“…In other countries of Southern European Coast, such as Turkey, chemical composition of propolis has widely investigated by several authors. Quercetin (26), rutin (48), chrysin (2), pinocembrin (3), pinostrobin chalcone (49) and pinobanksin (1) and its derivatives were the most repeated flavonoids found in various simples of propolis from Turkey using different method of analysis such as gas chromatography-mass spectrometry (GC/MS), high-performance liquid chromatography (HPLC) and ultrahigh-performance liquid chromatography with a linear ion trap-high resolution Orbitrap mass spectrometry system (UHPLC-LTQ/orbitrap/MS/MS), [73,[78][79][80][81][82][83][84][85][86][87][88][89][90][91][92][93][94][95] while Erdogan et al [96] have reported the presence of other flavonoids such as gallocatechin (50), epigallocatechin (51) and myricetin (52) in Anatolian propolis submitted to a pressurized liquid extraction. Other authors did not report the presence of flavonoids in Turkish propolis originating from Hatay, Ankara-Kazan and Mugla-Marmaris, [97 -100] at the same time they declared that terpenes such as αand β-eudesmol, isopimaric acid (42), dehydroabietic acid (53) and abietic acid (54) ( Figure 4) were included in the bioactive compounds of tested propolis samples.…”

Section: Southern European Coastmentioning

confidence: 99%

“…Propolis also found to responsible on changes in the levels of antioxidative proteins and proteins involved in ATP synthesis in Saccharomyces cerevisiae. [51,52,145,146] Different propolis extracts including Croatian, Greek and Cyprus (Levantine Coast) ones were shown to possess a variable antioxidant capacity due to its richness in bioactive compounds. [17,51,62,65 -67,77,146 -147] Jug et al [148] have found propolis from Dalmatia and Zagreb, Croatia, to be excellent as antioxidant agent based on the results of the antioxidant tests assayed.…”

Section: Southern European Coastmentioning

confidence: 99%

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Insight on Propolis from Mediterranean Countries: Chemical Composition, Biological Activities and Application Fields

El-Guendouz

Lyoussi

Miguel

2019

Chemistry & Biodiversity

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This review updates the information upon the chemical composition of propolis from all Mediterranean countries as well as their biological properties and applications. The non‐volatile fraction of propolis was characterized by the presence of phenolic acids and their esters and flavonoids. Nevertheless, in some countries, diterpenes were also present: Sicily (Italy), Croatia, Malta, Creta (Greece), Turkey, Cyprus, Egypt, Libya, Algeria and Morocco. The volatile fraction of propolis was characterized by the presence of benzoic acid and its esters, mono‐ and sesquiterpenes, being the oxygenated sesquiterpene β‐eudesmol characteristic of poplar propolis, whereas the hydrocarbon monoterpene α‐pinene has been related with the presence of conifers. Regardless the chemical composition, there are common biological properties attributed to propolis. Owing to these attributes, propolis has been target of study for applications in diverse areas, such as food, medicine and livestock.

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Section: Southern European Coastmentioning

confidence: 99%

Section: Southern European Coastmentioning

confidence: 99%

Section: Southern European Coastmentioning

confidence: 99%

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Insight on Propolis from Mediterranean Countries: Chemical Composition, Biological Activities and Application Fields

El-Guendouz

Lyoussi

Miguel

2019

Chemistry & Biodiversity

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“…In this method, the reagent thiobarbituric acid generates adduct with malondialdehyde, which is detectable spectrophotometrically at 532 nm. Besides the aforementioned method, cell viability assays are also employed in assessing oxidative damage in yeast, which evaluates the stress tolerance increase caused by treatment with antioxidant compounds [71]; mitochondrial function assays, since many apoptotic processes start in this organelle [72]; measurement of intracellular reactive oxygen species formation, using 2,7-dichloroluorescein as indicator [71,73]; protein carbonylation tests [74,75], which is also formed as consequence of oxidative damage; assessment of energetic metabolism and enzymatic activity associated with the stress response [67,74], among other methods.…”

Section: Antioxidant Activity Assaysmentioning

confidence: 99%

Honey as a Functional Food

Luchese¹,

Prudêncio²,

Guerra³

2017

Honey Analysis

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The most well-known functional properties of honey are its antioxidant and antimicrobial activities. The bioactive components of honey are afected by the lora from which it is produced and by geographical variations. Phenolic compounds promote, among other activities, high antioxidant action, being capable of minimizing intracellular oxidative damage associated with cellular aging, apoptosis and neurodegenerative diseases. A living cell system would provide a beter platform for determining antioxidant activity, since the bioactive honey compounds can act modulating antioxidant defense gene expression. Indeed, phenolic compounds, amino acids and reducing sugars are among the substances responsible for honey antioxidant activity. Most of phenolic compounds also exert antimicrobial activity against a number of pathogens and spoilage microorganisms. The antimicrobial activity of honey is also due to the action of enzymes. In addition, honey was found to contain lactic acid bacteria (LAB), which itself produce a myriad of active compounds that remain in variable amounts in mature honey. In addition, these antioxidant compounds might play a key role as prebiotic, protecting and stimulating growth of probiotic bacteria. Oligosaccharides present in honey are well-known prebiotic substances stimulating growth, activity and protecting probiotic bacteria during passage through the gastrointestinal tract and during storage of the products. This chapter describes the main bioactive components of honey, especially with respect to the phenolic compounds and their antioxidant activity and assay methods.

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“…Regarding antioxidant activity at a cellular level, compounds showing antioxidant activity in vitro or in foods do not necessarily possess the same activity in the cells (21). If not injected, there will always be a GI digestion step, where peptides will be modifi ed, e.g.…”

Section: Antioxidant Activity In the Cells And Cellular Metabolic Energymentioning

confidence: 99%

Bioactivity of Cod and Chicken Protein Hydrolysates before and after in vitro Gastrointestinal Digestion

Jamnik

Istenič

Koštomaj

et al. 2017

Food Technol. Biotechnol.

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SummaryBioactivity of cod (Gadus morhua) and chicken (Gallus domesticus) protein hydrolysates before and aft er in vitro gastrointestinal (GI) digestion was investigated using yeast Saccharomyces cerevisiae as a model organism. Both hydrolysates were exposed to in vitro GI digestion prior to cellular exposure to simulate digestion conditions in the human body and therefore investigate the role of modulations in the GI tract on the cell response. The eff ect of digested and undigested hydrolysates on intracellular oxidation, cellular metabolic energy and proteome level was investigated. No diff erence in the eff ect on intracellular oxidation activity was obtained between cod and chicken hydrolysates, while higher aff ect on intracellular oxidation was provided by digested hydrolysates, with relative values of intracellular oxidation of cod of (70.2±0.8) and chicken of (74.5±1.4) % than by undigested ones, where values of cod and chicken were (95.5±1.2) and (90.5±0.7) %, respectively. Neither species nor digestion had any eff ect on cellular metabolic energy. At proteome level, digested hydrolysates gave again signifi cantly stronger responses than undigested counterparts; cod peptides here also gave somewhat stronger response than chicken peptides. The knowledge of the action of food protein hydrolysates and their digests within live cells, also at proteome level, is important for further validation of their activity in higher eukaryotes to develop new functional food ingredients, such as in this case chicken and cod muscle-derived peptides.

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Fractionation of Phenolic Compounds Extracted from Propolis and Their Activity in the Yeast Saccharomyces cerevisiae

Cited by 18 publications

References 23 publications

Insight on Propolis from Mediterranean Countries: Chemical Composition, Biological Activities and Application Fields

Insight on Propolis from Mediterranean Countries: Chemical Composition, Biological Activities and Application Fields

Honey as a Functional Food

Bioactivity of Cod and Chicken Protein Hydrolysates before and after in vitro Gastrointestinal Digestion

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