Stimulatory Effect of Different Lignocellulosic Materials for Phenolic Compound Production and Antioxidant Activity from Inonotus obliquus in Submerged Fermentation

Abstract

White-rot fungus Inonotus obliquus grown in submerged culture produces antioxidative phenolic compounds. In this study, addition of lignocellulosic materials into the liquid culture increased the production and antioxidant activity of extra- and intra-cellular phenolic compounds (EPC and IPC, respectively). The production of EPC and IPC was significantly enhanced by wheat straw (by 151.2 and 45.3 %), sugarcane bagasse (by 106.9 and 26.1 %), and rice straw (by 67.6 and 38.9 %). Both of the EPC and IPC extracts from the three substrates showed a higher hydroxyl and 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity than those from the control medium. The highly active polyphenols such as tea catechins of epicatechin-3-gallate (ECG) and epigallocatechin-3-gallate (EGCG), and phelligridin G in the EPC extracts increased by 113.1, 75.0, and 86.3 % in the sugarcane bagasse medium. Davallialactone and inoscavin B in the EPC extracts were generated in large amounts in the lignocellulose media but not found in the control medium. The IPC extract from the wheat straw medium had the highest production of EGCG and ECG (17.6 and 18.1 mg/l). The different enhancement among the materials was attributed to the content and degradation rate of cellulose, hemicellulose, and lignin. The different antioxidant activity of the EPC and IPC extracts was related to their phenolic compositions.

Tapping the Bioactivity Potential of Residual Stream from Its Pretreatments May Be a Green Strategy for Low-Cost Bioconversion of Rice Straw

“…However, it should be noted that phenolic compounds obtained via chemical methods would have fewer applications in the food and pharmaceutical industries than those obtained via biological methods. Therefore, the possibility of the use of phenolic compounds as antimicrobial agents was investigated [ 35 , 36 ]. Moreover, phenolic compounds could be used as biological pesticides because they could be produced in large quantities due to the huge amounts of available rice straw.…”

Stimulated production of steroids in Inonotus obliquus by host factors from birch

“…Thus, a great demand for the sclerotia of I. obliquus is observed because of several characteristics, such as rarity in nature, and complex cultivation. Submerged fermentation of I. obliquus has received great attention and perceived as a promising alternative for efficient production of mycelial biomass, phenolic compound, and exopolysaccharides (18,19). However, I. obliquus in submerged culture accumulates less bioactive metabolites than those grow in natural habitats (20).…”

“…However, I. obliquus in submerged culture accumulates less bioactive metabolites than those grow in natural habitats (20). To accelerate the production of metabolites by I. obliquus, effect of different lignocellulosic materials have been studied (18,21). Other studies also reported that the extracts from host-related species can stimulate mycelial biomass and metabolite production of medicinal mushroom such as Ganoderma lucidum (22,23).…”

Kinetic models for the effect of temperature on flavonoid production in liquid submerged fermentation by Phellinus baumii

“…Submerged fermentation provides a number of potential advantages, for instance a greater mycelial biomass and secondary metabolite production in a more‐compact space over a shorter time with a lower chance of contamination, availability of convenient control, and easy fermentation processing . Many investigators have verified optimal medium contribute to the flavonoid production by submerged fermentation . Additionally, the secondary metabolites products were obtained from liquid submerged fermentation and exhibited almost the same biological activities as those of fruiting body .…”